LOCUS BBa_I53422 2988 bp DNA linear 25-Nov-2024 DEFINITION pSC101.Amp ACCESSION BBa_I53422 VERSION BBa_I53422.1 FEATURES Location/Qualifiers stem_loop 46..75 /label=Terminator misc_feature 137..504 /label=par region misc_feature 139..152 /label=inverted repeat misc_feature 158..162 /label=Tn3 integration site misc_feature 165..181 /label=inverted repeat misc_feature 192..215 /label=inverted repeat misc_feature 221..238 /label=inverted repeat misc_feature 635..717 /label=AT rich region misc_feature 716..735 /label=direct repeat misc_feature 737..756 /label=direct repeat misc_feature 769..788 /label=direct repeat misc_feature 814..819 /label=mutated SpeI site promoter 828..833 /label=-35 misc_feature 838..859 /label=inverted repeat promoter 846..865 /label=promoter region promoter 850..855 /label=-10 misc_feature 862..884 /label=inverted repeat RBS 870..882 /label=RBS RBS 879..884 /label=RBS CDS 886..1836 /label=repA misc_feature 1655..1655 /label=G to A difference with canonical pSC101 vector sequence misc_feature 1750..1750 /label=C to T difference with canonical pSC101 vector sequence misc_feature 1847..1861 /label=inverted repeat misc_feature 1912..1912 /label=G to C difference with canonical pSC101 vector sequence polyA_site 1947..1962 /label=putative polyA misc_feature 1963..1979 /label=inverted repeat misc_feature 2002..2002 /label=T to C difference with canonical pSC101 vector sequence misc_feature 1..2037 /label=BBa_I50042 CDS 2125..2988 /label=ampicillin resistance promoter 2076..2081 /label=ampR -35 promoter 2102..2107 /label=ampR -10 RBS 2116..2120 /label=ampR RBS misc_feature 2046..2988 /label=BBa_P1002 ORIGIN 1 ctgtcagacc aagtttacga gctcgcttgg actcctgttg atagatccag taatgacctc 61 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga 121 gaatccaagc actagggaca gtaagacggg taagcctgtt gatgataccg ctgccttact 181 gggtgcatta gccagtctga atgacctgtc acgggataat ccgaagtggt cagactggaa 241 aatcagaggg caggaactgc tgaacagcaa aaagtcagat agcaccacat agcagacccg 301 ccataaaacg ccctgagaag cccgtgacgg gcttttcttg tattatgggt agtttccttg 361 catgaatcca taaaaggcgc ctgtagtgcc atttaccccc attcactgcc agagccgtga 421 gcgcagcgaa ctgaatgtca cgaaaaagac agcgactcag gtgcctgatg gtcggagaca 481 aaaggaatat tcagcgattt gcccgagctt gcgagggtgc tacttaagcc tttagggttt 541 taaggtctgt tttgtagagg agcaaacagc gtttgcgaca tccttttgta atactgcgga 601 actgactaaa gtagtgagtt atacacaggg ctgggatcta ttctttttat ctttttttat 661 tctttcttta ttctataaat tataaccact tgaatataaa caaaaaaaac acacaaaggt 721 ctagcggaat ttacagaggg tctagcagaa tttacaagtt ttccagcaaa ggtctagcag 781 aatttacaga tacccacaac tcaaaggaaa aggacatgta attatcattg actagcccat 841 ctcaattggt atagtgatta aaatcaccta gaccaattga gatgtatgtc tgaattagtt 901 gttttcaaag caaatgaact agcgattagt cgctatgact taacggagca tgaaaccaag 961 ctaattttat gctgtgtggc actactcaac cccacgattg aaaaccctac aaggaaagaa 1021 cggacggtat cgttcactta taaccaatac gctcagatga tgaacatcag tagggaaaat 1081 gcttatggtg tattagctaa agcaaccaga gagctgatga cgagaactgt ggaaatcagg 1141 aatcctttgg ttaaaggctt tgagattttc cagtggacaa actatgccaa gttctcaagc 1201 gaaaaattag aattagtttt tagtgaagag atattgcctt atcttttcca gttaaaaaaa 1261 ttcataaaat ataatctgga acatgttaag tcttttgaaa acaaatactc tatgaggatt 1321 tatgagtggt tattaaaaga actaacacaa aagaaaactc acaaggcaaa tatagagatt 1381 agccttgatg aatttaagtt catgttaatg cttgaaaata actaccatga gtttaaaagg 1441 cttaaccaat gggttttgaa accaataagt aaagatttaa acacttacag caatatgaaa 1501 ttggtggttg ataagcgagg ccgcccgact gatacgttga ttttccaagt tgaactagat 1561 agacaaatgg atctcgtaac cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa 1621 ataccaacaa ccattacatc agattcctac ctacataacg gactaagaaa aacactacac 1681 gatgctttaa ctgcaaaaat tcagctcacc agttttgagg caaaattttt gagtgacatg 1741 caaagtaagt atgatctcaa tggttcgttc tcatggctca cgcaaaaaca acgaaccaca 1801 ctagagaaca tactggctaa atacggaagg atctgaggtt cttatggctc ttgtatctat 1861 cagtgaagca tcaagactaa caaacaaaag tagaacaact gttcaccgtt acatatcaaa 1921 gggaaaactg tccatatgca cagatgaaaa cggtgtaaaa aagatagata catcagagct 1981 tttacgagtt tttggtgcat tcaaagctgt tcaccatgaa cagatcgaca atgtaactac 2041 tagagctaaa tacattcaaa tatctatccg ctcatgagac aataaccctg ataaatgctt 2101 caataatatt gaaaaaggaa gaatatgagt attcaacatt tccgtgtcgc ccttattccc 2161 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 2221 gatgccgaag atcagttggg tgcacgtgtg ggttacatcg aactggacct caacagcggt 2281 aagattcttg agagttttcg ccccgaagaa cgtttcccaa tgatgagcac ttttaaagtt 2341 ctgctctgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 2401 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 2461 gacggcatga cagtacgcga attatgcagc gctgccataa ccatgagtga taacacggcg 2521 gccaacttac ttctgacaac gatcggagga ccgaaggagc ttaccgcttt tttgcacaac 2581 atgggtgatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 2641 aacgacgagc gtgacaccac gatgcctgta gctatggcaa caacgttgcg caaactctta 2701 actggcgaac ttcttactct cgcttcccgg caacaattaa tagactggat ggaggcggat 2761 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 2821 tctggagccg gtgagcgtgg gtcccgcggt attattgcag ccctggggcc agatggtaag 2881 ccctcccgta tcgtagttat ctacacgacg gggagccagg caactatgga cgaacgtaat 2941 cgccagatcg ctgagatagg tgcctccctg attaagcatt ggtaataa //