##gff-version 3
##sequence-region BBa_I53422 1 2988
BBa_I53422	.	sequence_feature	1	2037	.	+	0	ID=BBa_I50042;Name=BBa_I50042
BBa_I53422	.	sequence_feature	838	859	.	+	0	ID=annotation2071322;Name=inverted repeat;Parent=BBa_I50042
BBa_I53422	.	promoter	846	865	.	+	0	ID=annotation2071323;Name=promoter region;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	862	884	.	+	0	ID=annotation2071325;Name=inverted repeat;Parent=BBa_I50042
BBa_I53422	.	sequence_alteration	1912	1912	.	+	0	ID=annotation2071332;Name=G to C difference with canonical pSC101 vector sequence;Parent=BBa_I50042
BBa_I53422	.	sequence_alteration	2002	2002	.	+	0	ID=annotation2071335;Name=T to C difference with canonical pSC101 vector sequence;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	165	181	.	+	0	ID=annotation2071313;Name=inverted repeat;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	221	238	.	+	0	ID=annotation2071315;Name=inverted repeat;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	139	152	.	+	0	ID=annotation2071311;Name=inverted repeat;Parent=BBa_I50042
BBa_I53422	.	promoter	828	833	.	+	0	ID=annotation2071321;Name=-35;Parent=BBa_I50042
BBa_I53422	.	promoter	850	855	.	+	0	ID=annotation2071324;Name=-10;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	158	162	.	+	0	ID=annotation2071312;Name=Tn3 integration site;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	1963	1979	.	+	0	ID=annotation2071334;Name=inverted repeat;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	1847	1861	.	+	0	ID=annotation2071331;Name=inverted repeat;Parent=BBa_I50042
BBa_I53422	.	sequence_alteration	814	819	.	+	0	ID=annotation2071320;Name=mutated SpeI site;Parent=BBa_I50042
BBa_I53422	.	polyA_site	1947	1962	.	+	0	ID=annotation2071333;Name=putative polyA;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	192	215	.	+	0	ID=annotation2071314;Name=inverted repeat;Parent=BBa_I50042
BBa_I53422	.	CDS	886	1836	.	+	0	ID=annotation2071328;Name=repA;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	737	756	.	+	0	ID=annotation2071318;Name=direct repeat;Parent=BBa_I50042
BBa_I53422	.	ribosome_entry_site	879	884	.	+	0	ID=annotation2071327;Name=RBS;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	716	735	.	+	0	ID=annotation2071317;Name=direct repeat;Parent=BBa_I50042
BBa_I53422	.	sequence_alteration	1750	1750	.	+	0	ID=annotation2071330;Name=C to T difference with canonical pSC101 vector sequence;Parent=BBa_I50042
BBa_I53422	.	ribosome_entry_site	870	882	.	+	0	ID=annotation2071326;Name=RBS;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	137	504	.	+	0	ID=annotation2071310;Name=par region;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	769	788	.	+	0	ID=annotation2071319;Name=direct repeat;Parent=BBa_I50042
BBa_I53422	.	stem_loop	46	75	.	+	0	ID=annotation2071309;Name=Terminator;Parent=BBa_I50042
BBa_I53422	.	sequence_feature	635	717	.	+	0	ID=annotation2071316;Name=AT rich region;Parent=BBa_I50042
BBa_I53422	.	sequence_alteration	1655	1655	.	+	0	ID=annotation2071329;Name=G to A difference with canonical pSC101 vector sequence;Parent=BBa_I50042
BBa_I53422	.	engineered_region	2046	2988	.	+	0	ID=BBa_P1002;Name=BBa_P1002
BBa_I53422	.	promoter	2076	2081	.	+	0	ID=annotation1897109;Name=ampR -35;Parent=BBa_P1002
BBa_I53422	.	CDS	2125	2988	.	+	0	ID=annotation1790786;Name=ampicillin resistance;Parent=BBa_P1002
BBa_I53422	.	ribosome_entry_site	2116	2120	.	+	0	ID=annotation1897111;Name=ampR RBS;Parent=BBa_P1002
BBa_I53422	.	promoter	2102	2107	.	+	0	ID=annotation1897110;Name=ampR -10;Parent=BBa_P1002
>BBa_I53422
ctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttg
ttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctg
ttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactg
gaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctga
gaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccattta
cccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcg
gagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgtttt
gtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggct
gggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaaca
cacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacaga
tacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacct
agaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggag
catgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacgg
tatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaac
cagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactat
gccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattca
taaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaact
aacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataac
taccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaat
tggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaac
cgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataac
ggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgaca
tgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggc
taaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaac
aactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatca
gagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaactactagagctaaataca
ttcaaatatctatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagaatatgagtatt
caacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtga
aagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttga
gagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtatt
gacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaa
agcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaactt
acttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgat
cgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgt
tgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagt
tgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcc
cgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaa
ctatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataa