Ampicillin resistance cassette for modular BioBricks vector construction.
This part was amplified from pSB4A3-P1010.
pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids.
The replication origin boundaries were chosen quite liberally (i.e. they might include more sequence than is strictly necessary) because a different version of this origin (BBa_I50040) was shown to be non-functional.
G to A difference with canonical pSC101 vector sequence
C to T difference with canonical pSC101 vector sequence
G to C difference with canonical pSC101 vector sequence
T to C difference with canonical pSC101 vector sequence
Conversion of the iGEM parts registry to SBOL2.1