##gff-version 3
##sequence-region BBa_I5410 1 3117
BBa_I5410	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I5410	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I5410	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I5410	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I5410	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I5410	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I5410	.	terminator	1728	1773	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5410	.	engineered_region	1728	1773	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5410	.	stem_loop	1740	1762	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5410	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5410	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5410	.	terminator	833	878	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5410	.	engineered_region	833	878	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5410	.	stem_loop	845	867	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5410	.	terminator	1679	1719	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5410	.	stop_codon	1712	1712	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5410	.	stem_loop	1686	1705	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5410	.	polyA_site	1706	1719	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5410	.	engineered_region	1679	1719	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5410	.	CDS	1862	2989	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I5410	.	engineered_tag	2990	3014	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I5410	.	engineered_region	1862	2989	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I5410	.	tag	2951	2989	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I5410	.	CDS	1862	2989	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I5410	.	CDS	82	750	.	+	0	ID=BBa_C0052;Name=BBa_C0052
BBa_I5410	.	CDS	82	750	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_I5410	.	tag	712	750	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_I5410	.	engineered_region	82	750	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_I5410	.	engineered_tag	751	775	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_I5410	.	terminator	3023	3063	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5410	.	stop_codon	3056	3056	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5410	.	stem_loop	3030	3049	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5410	.	polyA_site	3050	3063	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5410	.	engineered_region	3023	3063	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5410	.	promoter	887	932	.	+	0	ID=BBa_R0052;Name=BBa_R0052
BBa_I5410	.	promoter	887	892	.	+	0	ID=annotation2029;Name=-35;Parent=BBa_R0052
BBa_I5410	.	promoter	910	915	.	+	0	ID=annotation2030;Name=-10;Parent=BBa_R0052
BBa_I5410	.	promoter	888	893	.	+	0	ID=annotation2031;Name=-35;Parent=BBa_R0052
BBa_I5410	.	non_covalent_binding_site	894	907	.	+	0	ID=annotation2027;Name=OR2;Parent=BBa_R0052
BBa_I5410	.	start_codon	921	923	.	+	0	ID=annotation2032;Name=start;Parent=BBa_R0052
BBa_I5410	.	engineered_region	887	932	.	+	0	ID=annotation7068;Name=BBa_R0052;Parent=BBa_R0052
BBa_I5410	.	non_covalent_binding_site	916	929	.	+	0	ID=annotation2028;Name=OR1;Parent=BBa_R0052
BBa_I5410	.	non_covalent_binding_site	887	929	.	+	0	ID=annotation2026;Name=OR2;Parent=BBa_R0052
BBa_I5410	.	promoter	1782	1835	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_I5410	.	non_covalent_binding_site	1815	1832	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I5410	.	non_covalent_binding_site	1792	1809	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I5410	.	non_covalent_binding_site	1782	1784	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I5410	.	promoter	1799	1804	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I5410	.	engineered_region	1782	1835	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I5410	.	promoter	1811	1816	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I5410	.	promoter	1789	1794	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I5410	.	ribosome_entry_site	941	952	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5410	.	conserved_region	945	948	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5410	.	terminator	3072	3117	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5410	.	engineered_region	3072	3117	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5410	.	stem_loop	3084	3106	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5410	.	CDS	959	1645	.	+	0	ID=BBa_C0053;Name=BBa_C0053
BBa_I5410	.	stop_codon	1640	1645	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_I5410	.	engineered_tag	1646	1670	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_I5410	.	tag	1607	1639	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_I5410	.	engineered_region	959	1645	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_I5410	.	CDS	959	1606	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_I5410	.	terminator	784	824	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5410	.	stop_codon	817	817	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5410	.	stem_loop	791	810	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5410	.	polyA_site	811	824	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5410	.	engineered_region	784	824	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5410	.	ribosome_entry_site	1844	1855	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5410	.	conserved_region	1848	1851	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I5410
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggg
gactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagct
cttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaag
ggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattga
tgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacct
gtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtag
ccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaa
tccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgta
gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggctttt
ttattattttactagagttgacaaacaagatacattgtatgaaaatacaagaaagtttgttgatactagagaaagagga
gaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgct
cttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacc
tgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgccta
tcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaa
ccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtcc
aaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaacc
aagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggc
cgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttg
ttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgcta
gtgtagatcactactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataa
aaagccagattattaatccggcttttttattattttactagagaataaacttgactaaagattcctttagtagataatt
taagtgttctttaatttctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgt
cgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgg
gaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgt
tgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccga
tcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatctt
ctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgca
ctaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtac
gcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcg
gcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgact
ggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaa
cgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtggga
tacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaa
ccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaa
aagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacga
caggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgct
agtgtagatctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatata
aaaagccagattattaatccggcttttttattattt