LOCUS BBa_I5500 3685 bp DNA linear 21-Dec-2024 DEFINITION Olmecs: Synchro-Dualator part 1 ACCESSION BBa_I5500 VERSION BBa_I5500.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 831..855 /label=Help:Barcodes CDS 81..830 /label=BBa_C0051 stem_loop 871..890 /label=T7 TE misc_feature 897..897 /label=stop polyA_site 891..904 /label=polya misc_feature 864..904 /label=BBa_B0012 terminator 864..904 /label=BBa_B0012 stem_loop 925..947 /label=stem_loop misc_feature 913..958 /label=BBa_B0011 terminator 913..958 /label=BBa_B0011 promoter 1004..1009 /label=-10 promoter 981..986 /label=-35 misc_feature 991..1007 /label=OR1 misc_feature 967..983 /label=OR2 misc_feature 967..1015 /label=BBa_R0051 promoter 967..1015 /label=BBa_R0051 misc_feature 1028..1031 /label=conserved RBS 1024..1035 /label=BBa_B0034 CDS 1042..1689 /label=cII p22 misc_feature 1690..1722 /label=LVA misc_feature 1723..1728 /label=stop misc_feature 1042..1728 /label=BBa_C0053 misc_feature 1729..1753 /label=Help:Barcodes CDS 1042..1728 /label=BBa_C0053 stem_loop 1769..1788 /label=T7 TE misc_feature 1795..1795 /label=stop polyA_site 1789..1802 /label=polya misc_feature 1762..1802 /label=BBa_B0012 terminator 1762..1802 /label=BBa_B0012 stem_loop 1823..1845 /label=stem_loop misc_feature 1811..1856 /label=BBa_B0011 terminator 1811..1856 /label=BBa_B0011 misc_feature 1865..1867 /label=OR3 misc_feature 1875..1892 /label=OR2 misc_feature 1898..1915 /label=OR1 promoter 1882..1887 /label=-35 promoter 1872..1877 /label=-35 promoter 1894..1899 /label=-10 misc_feature 1865..1918 /label=BBa_R0053 promoter 1865..1918 /label=BBa_R0053 misc_feature 1931..1934 /label=conserved RBS 1927..1938 /label=BBa_B0034 CDS 1948..2564 /label=tetR misc_feature 2565..2598 /label=SsrA misc_feature 2605..2629 /label=Help:Barcodes CDS 1945..2604 /label=BBa_C0040 stem_loop 2645..2664 /label=T7 TE misc_feature 2671..2671 /label=stop polyA_site 2665..2678 /label=polya misc_feature 2638..2678 /label=BBa_B0012 terminator 2638..2678 /label=BBa_B0012 stem_loop 2699..2721 /label=stem_loop misc_feature 2687..2732 /label=BBa_B0011 terminator 2687..2732 /label=BBa_B0011 misc_feature 2741..2759 /label=TetR 1 misc_feature 2741..2794 /label=BBa_R0040 promoter 2760..2765 /label=-35 misc_feature 2766..2784 /label=TetR 2 promoter 2783..2788 /label=-10 promoter 2741..2794 /label=BBa_R0040 misc_feature 2807..2810 /label=conserved RBS 2803..2814 /label=BBa_B0034 CDS 2821..3582 /label=CFP (LVA) misc_feature 3539..3576 /label=SsrA misc_feature 3577..3582 /label=2 misc_feature 2889..2889 /label=A misc_feature 2821..3582 /label=BBa_E0022 misc_feature 2821..3582 /label=BBa_E0022 stem_loop 3598..3617 /label=T7 TE misc_feature 3624..3624 /label=stop polyA_site 3618..3631 /label=polya misc_feature 3591..3631 /label=BBa_B0012 terminator 3591..3631 /label=BBa_B0012 stem_loop 3652..3674 /label=stem_loop misc_feature 3640..3685 /label=BBa_B0011 terminator 3640..3685 /label=BBa_B0011 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 901 tatatactag agagagaata taaaaagcca gattattaat ccggcttttt tattatttta 961 ctagagtaac accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgctacta 1021 gagaaagagg agaaatacta gatgaataca caattgatgg gtgagcgtat tcgcgctcga 1081 agaaaaaaac tcaagattag acaagccgct cttggtaaga tggtgggagt gtctaatgtt 1141 gcaatatcgc aatgggagcg ctcggagact gagccaaatg gggagaacct gttggcactt 1201 tcgaaggctc ttcagtgctc ccctgactat ttgctgaaag gagatttaag ccagacaaac 1261 gttgcctatc atagtaggca tgagccaaga ggatcatacc ctcttatcag ttgggtaagc 1321 gcagggcaat ggatggaagc tgtagaacct tatcacaagc gcgcgataga gaactggcac 1381 gacaccactg tagattgttc agaagattca ttttggcttg atgtccaagg tgactctatg 1441 acagcaccgg cagggttaag cattccagaa ggaatgataa ttctggttga tcccgaagtc 1501 gaaccaagaa acggcaagct ggttgttgca aaattagaag gtgaaaacga ggccacattc 1561 aaaaaattag ttatggatgc aggccgaaag tttttaaaac cattaaaccc acaatatccg 1621 atgatagaaa tcaacggaaa ctgcaaaatc attggcgtag ttgttgacgc aaaactcgca 1681 aatcttccag ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 1741 tagtgtagat cactactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 1801 tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta ttattttact 1861 agagaataaa cttgactaaa gattccttta gtagataatt taagtgttct ttaatttcta 1921 ctagagaaag aggagaaata ctagatgtcc agattagata aaagtaaagt gattaacagc 1981 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag 2041 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc 2101 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg 2161 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt 2221 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact 2281 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat 2341 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa 2401 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa 2461 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg 2521 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa 2581 aactacgctt tagtagctta ataacactga tagtgctagt gtagatcact actagagtca 2641 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa 2701 gccagattat taatccggct tttttattat tttactagag tccctatcag tgatagagat 2761 tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag 2821 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 2881 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 2941 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 3001 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag 3061 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 3121 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 3181 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 3241 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac 3301 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 3361 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 3421 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 3481 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagg 3541 cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag tcacactggc 3601 tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat 3661 tattaatccg gcttttttat tattt //