LOCUS BBa_I5510 4071 bp DNA linear 16-Apr-2024 DEFINITION Olmecs: Synchro-Dualator part 2 ACCESSION BBa_I5510 VERSION BBa_I5510.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..750 /label=cI 434 misc_feature 712..750 /label=LVA misc_feature 82..750 /label=BBa_C0052 misc_feature 751..775 /label=Help:Barcodes CDS 82..750 /label=BBa_C0052 stem_loop 791..810 /label=T7 TE misc_feature 817..817 /label=stop polyA_site 811..824 /label=polya misc_feature 784..824 /label=BBa_B0012 terminator 784..824 /label=BBa_B0012 stem_loop 845..867 /label=stem_loop misc_feature 833..878 /label=BBa_B0011 terminator 833..878 /label=BBa_B0011 misc_feature 887..929 /label=OR2 misc_feature 894..907 /label=OR2 misc_feature 916..929 /label=OR1 promoter 887..892 /label=-35 promoter 910..915 /label=-10 promoter 888..893 /label=-35 misc_feature 921..923 /label=start misc_feature 887..932 /label=BBa_R0052 promoter 887..932 /label=BBa_R0052 misc_feature 945..948 /label=conserved RBS 941..952 /label=BBa_B0034 CDS 959..1606 /label=cII p22 misc_feature 1607..1639 /label=LVA misc_feature 1640..1645 /label=stop misc_feature 959..1645 /label=BBa_C0053 misc_feature 1646..1670 /label=Help:Barcodes CDS 959..1645 /label=BBa_C0053 stem_loop 1686..1705 /label=T7 TE misc_feature 1712..1712 /label=stop polyA_site 1706..1719 /label=polya misc_feature 1679..1719 /label=BBa_B0012 terminator 1679..1719 /label=BBa_B0012 stem_loop 1740..1762 /label=stem_loop misc_feature 1728..1773 /label=BBa_B0011 terminator 1728..1773 /label=BBa_B0011 misc_feature 1782..1784 /label=OR3 misc_feature 1792..1809 /label=OR2 misc_feature 1815..1832 /label=OR1 promoter 1799..1804 /label=-35 promoter 1789..1794 /label=-35 promoter 1811..1816 /label=-10 misc_feature 1782..1835 /label=BBa_R0053 promoter 1782..1835 /label=BBa_R0053 misc_feature 1848..1851 /label=conserved RBS 1844..1855 /label=BBa_B0034 misc_feature 2951..2989 /label=LVA CDS 1862..2989 /label=lacI-LVA misc_feature 1862..2989 /label=BBa_C0012 misc_feature 2990..3014 /label=Help:Barcodes CDS 1862..2989 /label=BBa_C0012 stem_loop 3030..3049 /label=T7 TE misc_feature 3056..3056 /label=stop polyA_site 3050..3063 /label=polya misc_feature 3023..3063 /label=BBa_B0012 terminator 3023..3063 /label=BBa_B0012 stem_loop 3084..3106 /label=stem_loop misc_feature 3072..3117 /label=BBa_B0011 terminator 3072..3117 /label=BBa_B0011 misc_feature 1..3117 /label=BBa_I5410 misc_feature 3128..3144 /label=lac O1 promoter 3145..3150 /label=-35 misc_feature 3151..3167 /label=lac O1 promoter 3168..3173 /label=-10 misc_feature 3126..3179 /label=BBa_R0011 promoter 3126..3179 /label=BBa_R0011 misc_feature 3193..3196 /label=conserved RBS 3189..3200 /label=BBa_B0034 CDS 3207..3968 /label=YFP (LVA) misc_feature 3963..3968 /label=2 misc_feature 3924..3962 /label=SsrA misc_feature 3448..3448 /label=A (Q->L) misc_feature 3207..3968 /label=BBa_E0032 misc_feature 3207..3968 /label=BBa_E0032 stem_loop 3984..4003 /label=T7 TE misc_feature 4010..4010 /label=stop polyA_site 4004..4017 /label=polya misc_feature 3977..4017 /label=BBa_B0012 terminator 3977..4017 /label=BBa_B0012 stem_loop 4038..4060 /label=stem_loop misc_feature 4026..4071 /label=BBa_B0011 terminator 4026..4071 /label=BBa_B0011 misc_feature 3126..4071 /label=BBa_I5310 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagtatt tcttccaggg taaaaagcaa aagaatccag 121 cttggactta accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag 181 cagctcgaaa acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt 241 ggcgtaagtg ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg 301 cacgttgagc ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt 361 gaagcttgtg aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac 421 ttattaggca atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt 481 caaagcatcc ctgaaggtca tatggtgtta gtagatactg gacgggagcc agtgaatgga 541 agccttgttg tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata 601 gatggcggtc agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac 661 ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt agctgcaaac 721 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta 781 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata 841 taaaaagcca gattattaat ccggcttttt tattatttta ctagagttga caaacaagat 901 acattgtatg aaaatacaag aaagtttgtt gatactagag aaagaggaga aatactagat 961 gaatacacaa ttgatgggtg agcgtattcg cgctcgaaga aaaaaactca agattagaca 1021 agccgctctt ggtaagatgg tgggagtgtc taatgttgca atatcgcaat gggagcgctc 1081 ggagactgag ccaaatgggg agaacctgtt ggcactttcg aaggctcttc agtgctcccc 1141 tgactatttg ctgaaaggag atttaagcca gacaaacgtt gcctatcata gtaggcatga 1201 gccaagagga tcataccctc ttatcagttg ggtaagcgca gggcaatgga tggaagctgt 1261 agaaccttat cacaagcgcg cgatagagaa ctggcacgac accactgtag attgttcaga 1321 agattcattt tggcttgatg tccaaggtga ctctatgaca gcaccggcag ggttaagcat 1381 tccagaagga atgataattc tggttgatcc cgaagtcgaa ccaagaaacg gcaagctggt 1441 tgttgcaaaa ttagaaggtg aaaacgaggc cacattcaaa aaattagtta tggatgcagg 1501 ccgaaagttt ttaaaaccat taaacccaca atatccgatg atagaaatca acggaaactg 1561 caaaatcatt ggcgtagttg ttgacgcaaa actcgcaaat cttccagctg caaacgacga 1621 aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac tactagagtc 1681 acactggctc accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa 1741 agccagatta ttaatccggc ttttttatta ttttactaga gaataaactt gactaaagat 1801 tcctttagta gataatttaa gtgttcttta atttctacta gagaaagagg agaaatacta 1861 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 1921 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 1981 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 2041 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 2101 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 2161 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 2221 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 2281 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 2341 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 2401 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 2461 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 2521 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 2581 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 2641 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 2701 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 2761 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 2821 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 2881 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 2941 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 3001 ctagtgtaga tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3061 atatactaga gagagaatat aaaaagccag attattaatc cggctttttt attattttac 3121 tagagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca 3181 tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg 3241 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 3301 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 3361 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct 3421 tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag 3481 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 3541 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 3601 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 3661 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 3721 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 3781 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc 3841 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 3901 tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac gctttagtag 3961 cttaataata ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 4021 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt t //