LOCUS BBa_I6013 5273 bp DNA linear 26-Dec-2024 DEFINITION QPI Curve Test I0500.E0422.I0500.Q04510.E0432 ACCESSION BBa_I6013 VERSION BBa_I6013.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1998 /label=CFP (LVA) misc_feature 1955..1992 /label=SsrA misc_feature 1993..1998 /label=2 misc_feature 1305..1305 /label=A misc_feature 1237..1998 /label=BBa_E0022 misc_feature 1237..1998 /label=BBa_E0022 stem_loop 2018..2061 /label=stem_loop misc_feature 2007..2086 /label=BBa_B0010 terminator 2007..2086 /label=BBa_B0010 stem_loop 2102..2121 /label=T7 TE misc_feature 2128..2128 /label=stop polyA_site 2122..2135 /label=polya misc_feature 2095..2135 /label=BBa_B0012 terminator 2095..2135 /label=BBa_B0012 promoter 3223..3353 /label=Pbad promoter 3186..3257 /label=PC misc_feature 2144..3222 /label=AraC promoter 2144..3353 /label=BBa_I0500 misc_feature 3366..3369 /label=conserved RBS 3362..3373 /label=BBa_B0034 CDS 3383..4090 /label=cI lambda misc_feature 4091..4123 /label=LVA misc_feature 4130..4154 /label=Help:Barcodes CDS 3380..4129 /label=BBa_C0051 stem_loop 4174..4217 /label=stem_loop misc_feature 4163..4242 /label=BBa_B0010 terminator 4163..4242 /label=BBa_B0010 stem_loop 4258..4277 /label=T7 TE misc_feature 4284..4284 /label=stop polyA_site 4278..4291 /label=polya misc_feature 4251..4291 /label=BBa_B0012 terminator 4251..4291 /label=BBa_B0012 promoter 4337..4342 /label=-10 promoter 4314..4319 /label=-35 misc_feature 4324..4340 /label=OR1 misc_feature 4300..4316 /label=OR2 misc_feature 4300..4348 /label=BBa_R0051 promoter 4300..4348 /label=BBa_R0051 misc_feature 4361..4364 /label=conserved RBS 4357..4368 /label=BBa_B0034 CDS 4375..5136 /label=YFP (LVA) misc_feature 5131..5136 /label=2 misc_feature 5092..5130 /label=SsrA misc_feature 4616..4616 /label=A (Q->L) misc_feature 4375..5136 /label=BBa_E0032 misc_feature 4375..5136 /label=BBa_E0032 stem_loop 5156..5199 /label=stem_loop misc_feature 5145..5224 /label=BBa_B0010 terminator 5145..5224 /label=BBa_B0010 stem_loop 5240..5259 /label=T7 TE misc_feature 5266..5266 /label=stop polyA_site 5260..5273 /label=polya misc_feature 5233..5273 /label=BBa_B0012 terminator 5233..5273 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac 1981 gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2041 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2101 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttatgac aacttgacgg 2161 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc 2221 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg 2281 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct 2341 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg 2401 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat 2461 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt 2521 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg 2581 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg 2641 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa 2701 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag 2761 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg 2821 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat 2881 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg 2941 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg 3001 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt 3061 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca 3121 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa 3181 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg 3241 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac 3301 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agctactaga 3361 gaaagaggag aaatactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga 3421 ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca 3481 ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg 3541 catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt 3601 tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat 3661 gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat 3721 gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac 3781 caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc 3841 aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc 3901 tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa 3961 actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat 4021 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga 4081 gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg 4141 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 4201 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 4261 tcaccttcgg gtgggccttt ctgcgtttat atactagagt aacaccgtgc gtgttgacta 4321 ttttacctct ggcggtgata atggttgcta ctagagaaag aggagaaata ctagatggtg 4381 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 4441 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 4501 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 4561 accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac 4621 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 4681 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 4741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 4801 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 4861 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 4921 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 4981 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 5041 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaggcctgct 5101 gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa 5161 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 5221 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //