##gff-version 3
##sequence-region BBa_I6060 1 1125
BBa_I6060	.	engineered_region	227	988	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I6060	.	sequence_alteration	468	468	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I6060	.	engineered_region	227	988	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I6060	.	tag	944	982	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I6060	.	stop_codon	983	988	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I6060	.	CDS	227	988	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I6060	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I6060	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I6060	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I6060	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I6060	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I6060	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I6060	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I6060	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I6060	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I6060	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I6060	.	terminator	1085	1125	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I6060	.	stem_loop	1092	1111	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I6060	.	stop_codon	1118	1118	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I6060	.	polyA_site	1112	1125	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I6060	.	engineered_region	1085	1125	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I6060	.	terminator	997	1076	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I6060	.	stem_loop	1008	1051	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I6060	.	engineered_region	997	1076	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I6060
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggtgagcaa
gggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtg
tccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgc
cctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacga
cttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacc
cgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggca
acatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcat
caaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccccc
atcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgaga
agcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcc
tgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttata