##gff-version 3
##sequence-region BBa_I6074 1 1076
BBa_I6074	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I6074	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I6074	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I6074	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I6074	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I6074	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I6074	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I6074	.	terminator	1036	1076	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I6074	.	stem_loop	1043	1062	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I6074	.	stop_codon	1069	1069	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I6074	.	polyA_site	1063	1076	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I6074	.	engineered_region	1036	1076	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I6074	.	engineered_region	178	939	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I6074	.	CDS	178	939	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I6074	.	sequence_alteration	419	419	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I6074	.	engineered_region	178	939	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I6074	.	stop_codon	934	939	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I6074	.	tag	895	933	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I6074	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I6074	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I6074	.	terminator	948	1027	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I6074	.	stem_loop	959	1002	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I6074	.	engineered_region	948	1027	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I6074
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggac
ggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagt
tcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgc
ccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatc
ttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagc
tgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtcta
tatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcag
ctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctacc
agtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcac
tctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata