LOCUS       BBa_I6082                971 bp    DNA     linear       22-Nov-2024
DEFINITION  Promoter O_H Test R1052.E0432
ACCESSION   BBa_I6082
VERSION     BBa_I6082.1  
FEATURES             Location/Qualifiers
     promoter        1..43
                     /label=-10
     misc_feature    8..21
                     /label=OR2
     misc_feature    30..43
                     /label=OR1
     promoter        1..6
                     /label=-35
     promoter        2..7
                     /label=-35
     misc_feature    35..37
                     /label=start
     promoter        24..29
                     /label=-10
     misc_feature    1..46
                     /label=BBa_R1052
     promoter        1..46
                     /label=BBa_R1052
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             73..834
                     /label=YFP (LVA)
     misc_feature    829..834
                     /label=2
     misc_feature    790..828
                     /label=SsrA
     misc_feature    314..314
                     /label=A (Q->L)
     misc_feature    73..834
                     /label=BBa_E0032
     misc_feature    73..834
                     /label=BBa_E0032
     stem_loop       854..897
                     /label=stem_loop
     misc_feature    843..922
                     /label=BBa_B0010
     terminator      843..922
                     /label=BBa_B0010
     stem_loop       938..957
                     /label=T7 TE
     misc_feature    964..964
                     /label=stop
     polyA_site      958..971
                     /label=polya
     misc_feature    931..971
                     /label=BBa_B0012
     terminator      931..971
                     /label=BBa_B0012
ORIGIN
        1 ttgacaaaca agatacattg tatgatacta caagaaagtt tgttgatact agagaaagag
       61 gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      121 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      181 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      241 ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc ccgctacccc
      301 gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      361 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      421 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      481 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      541 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      601 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      661 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
      721 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      781 ctgtacaaga ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag
      841 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      901 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      961 ctgcgtttat a
//