##gff-version 3
##sequence-region BBa_I6082 1 971
BBa_I6082	.	engineered_region	73	834	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I6082	.	CDS	73	834	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I6082	.	sequence_alteration	314	314	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I6082	.	engineered_region	73	834	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I6082	.	stop_codon	829	834	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I6082	.	tag	790	828	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I6082	.	terminator	843	922	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I6082	.	stem_loop	854	897	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I6082	.	engineered_region	843	922	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I6082	.	ribosome_entry_site	55	66	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I6082	.	conserved_region	59	62	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I6082	.	promoter	1	46	.	+	0	ID=BBa_R1052;Name=BBa_R1052
BBa_I6082	.	promoter	24	29	.	+	0	ID=annotation2087;Name=-10;Parent=BBa_R1052
BBa_I6082	.	start_codon	35	37	.	+	0	ID=annotation2084;Name=start;Parent=BBa_R1052
BBa_I6082	.	promoter	1	43	.	+	0	ID=annotation2079;Name=-10;Parent=BBa_R1052
BBa_I6082	.	non_covalent_binding_site	8	21	.	+	0	ID=annotation2080;Name=OR2;Parent=BBa_R1052
BBa_I6082	.	promoter	1	6	.	+	0	ID=annotation2082;Name=-35;Parent=BBa_R1052
BBa_I6082	.	non_covalent_binding_site	30	43	.	+	0	ID=annotation2081;Name=OR1;Parent=BBa_R1052
BBa_I6082	.	promoter	2	7	.	+	0	ID=annotation2083;Name=-35;Parent=BBa_R1052
BBa_I6082	.	engineered_region	1	46	.	+	0	ID=annotation7075;Name=BBa_R1052;Parent=BBa_R1052
BBa_I6082	.	terminator	931	971	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I6082	.	stem_loop	938	957	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I6082	.	stop_codon	964	964	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I6082	.	polyA_site	958	971	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I6082	.	engineered_region	931	971	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_I6082
ttgacaaacaagatacattgtatgatactacaagaaagtttgttgatactagagaaagaggagaaatactagatggtga
gcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcag
cgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgccc
gtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgc
acgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaa
gacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggac
ggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacg
gcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacac
ccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaac
gagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaaga
ggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata