LOCUS       BBa_I6086                981 bp    DNA     linear       08-May-2024
DEFINITION  Promoter O_H Test R1062.E0432
ACCESSION   BBa_I6086
VERSION     BBa_I6086.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        42..48
                     /label=-10
     misc_feature    54..54
                     /label=start
     promoter        20..25
                     /label=-35
     misc_feature    1..56
                     /label=BBa_R1062
     promoter        1..56
                     /label=BBa_R1062
     misc_feature    69..72
                     /label=conserved
     RBS             65..76
                     /label=BBa_B0034
     CDS             83..844
                     /label=YFP (LVA)
     misc_feature    839..844
                     /label=2
     misc_feature    800..838
                     /label=SsrA
     misc_feature    324..324
                     /label=A (Q->L)
     misc_feature    83..844
                     /label=BBa_E0032
     misc_feature    83..844
                     /label=BBa_E0032
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt tgacacaaga aaatggtttg ttgatactcg aataaatact
       61 agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt
      121 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      181 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      241 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc
      301 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta
      361 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      421 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      481 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      541 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      601 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      661 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
      721 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      781 catggacgag ctgtacaaga ggcctgctgc aaacgacgaa aactacgctt tagtagctta
      841 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat a
//