##gff-version 3
##sequence-region BBa_I6086 1 981
BBa_I6086	.	terminator	853	932	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I6086	.	stem_loop	864	907	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I6086	.	engineered_region	853	932	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I6086	.	engineered_region	83	844	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I6086	.	sequence_alteration	324	324	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I6086	.	engineered_region	83	844	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I6086	.	tag	800	838	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I6086	.	stop_codon	839	844	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I6086	.	CDS	83	844	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I6086	.	ribosome_entry_site	65	76	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I6086	.	conserved_region	69	72	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I6086	.	promoter	1	56	.	+	0	ID=BBa_R1062;Name=BBa_R1062
BBa_I6086	.	engineered_region	1	56	.	+	0	ID=annotation7077;Name=BBa_R1062;Parent=BBa_R1062
BBa_I6086	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2098;Name=LuxR/HSL;Parent=BBa_R1062
BBa_I6086	.	start_codon	54	54	.	+	0	ID=annotation2100;Name=start;Parent=BBa_R1062
BBa_I6086	.	promoter	20	25	.	+	0	ID=annotation2101;Name=-35;Parent=BBa_R1062
BBa_I6086	.	promoter	42	48	.	+	0	ID=annotation2099;Name=-10;Parent=BBa_R1062
BBa_I6086	.	terminator	941	981	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I6086	.	stem_loop	948	967	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I6086	.	stop_codon	974	974	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I6086	.	polyA_site	968	981	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I6086	.	engineered_region	941	981	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_I6086
acctgtaggatcgtacaggttgacacaagaaaatggtttgttgatactcgaataaatactagagaaagaggagaaatac
tagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggcc
acaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccgg
caagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccac
atgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacg
gcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgactt
caaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaag
cagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacc
agcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaa
agaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgag
ctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata