LOCUS       BBa_I6201                978 bp    DNA     linear       08-May-2024
DEFINITION  Promoter O_H Test R0071.E0432
ACCESSION   BBa_I6201
VERSION     BBa_I6201.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     misc_feature    66..69
                     /label=conserved
     RBS             62..73
                     /label=BBa_B0034
     CDS             80..841
                     /label=YFP (LVA)
     misc_feature    836..841
                     /label=2
     misc_feature    797..835
                     /label=SsrA
     misc_feature    321..321
                     /label=A (Q->L)
     misc_feature    80..841
                     /label=BBa_E0032
     misc_feature    80..841
                     /label=BBa_E0032
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      121 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      181 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      241 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg
      301 ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt
      361 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      421 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      481 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      541 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      601 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      661 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
      721 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      781 ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata
      841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttata
//