LOCUS       BBa_I6213                970 bp    DNA     linear       22-Nov-2024
DEFINITION  Promoter O_H Test R2000.E0432
ACCESSION   BBa_I6213
VERSION     BBa_I6213.1  
FEATURES             Location/Qualifiers
     promoter        10..15
                     /label=-35
     promoter        33..38
                     /label=-10
     misc_feature    20..33
                     /label=Zif23
     misc_feature    1..9
                     /label=consensus
     misc_feature    45..45
                     /label=(+1)
     promoter        1..45
                     /label=BBa_R2000
     misc_feature    58..61
                     /label=conserved
     RBS             54..65
                     /label=BBa_B0034
     CDS             72..833
                     /label=YFP (LVA)
     misc_feature    828..833
                     /label=2
     misc_feature    789..827
                     /label=SsrA
     misc_feature    313..313
                     /label=A (Q->L)
     misc_feature    72..833
                     /label=BBa_E0032
     misc_feature    72..833
                     /label=BBa_E0032
     stem_loop       853..896
                     /label=stem_loop
     misc_feature    842..921
                     /label=BBa_B0010
     terminator      842..921
                     /label=BBa_B0010
     stem_loop       937..956
                     /label=T7 TE
     misc_feature    963..963
                     /label=stop
     polyA_site      957..970
                     /label=polya
     misc_feature    930..970
                     /label=BBa_B0012
     terminator      930..970
                     /label=BBa_B0012
ORIGIN
        1 agtttattct tgacatggtc ccacgcgcgt gggatactac gtcagtacta gagaaagagg
       61 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      121 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      181 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      241 cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg
      301 accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      361 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      421 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      481 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
      541 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
      601 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      661 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
      721 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
      781 tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga
      841 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      901 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      961 tgcgtttata
//