LOCUS BBa_I6300 4200 bp DNA linear 22-Nov-2024 DEFINITION QPI Curve Test I0500.E0420.I0500.E0430 ACCESSION BBa_I6300 VERSION BBa_I6300.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1959 /label=BBa_E0020 stem_loop 1979..2022 /label=stem_loop misc_feature 1968..2047 /label=BBa_B0010 terminator 1968..2047 /label=BBa_B0010 stem_loop 2063..2082 /label=T7 TE misc_feature 2089..2089 /label=stop polyA_site 2083..2096 /label=polya misc_feature 2056..2096 /label=BBa_B0012 terminator 2056..2096 /label=BBa_B0012 promoter 3184..3314 /label=Pbad promoter 3147..3218 /label=PC misc_feature 2105..3183 /label=AraC promoter 2105..3314 /label=BBa_I0500 misc_feature 3327..3330 /label=conserved RBS 3323..3334 /label=BBa_B0034 CDS 3341..4063 /label=BBa_E0030 stem_loop 4083..4126 /label=stem_loop misc_feature 4072..4151 /label=BBa_B0010 terminator 4072..4151 /label=BBa_B0010 stem_loop 4167..4186 /label=T7 TE misc_feature 4193..4193 /label=stop polyA_site 4187..4200 /label=polya misc_feature 4160..4200 /label=BBa_B0012 terminator 4160..4200 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagtaataat actagagcca ggcatcaaat 1981 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2041 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 2101 agagttatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact 2161 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa 2221 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg 2281 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga 2341 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa 2401 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca 2461 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca 2521 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc 2581 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg 2641 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc 2701 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg 2761 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc 2821 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa 2881 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa 2941 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc 3001 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt 3061 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa 3121 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag 3181 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa 3241 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt 3301 ttttttgggc tagctactag agaaagagga gaaatactag atggtgagca agggcgagga 3361 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 3421 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 3481 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta 3541 cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact tcttcaagtc 3601 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 3661 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 3721 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 3781 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 3841 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 3901 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc 3961 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 4021 cgccgggatc actctcggca tggacgagct gtacaagtaa taatactaga gccaggcatc 4081 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 4141 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //