LOCUS BBa_I6304 5724 bp DNA linear 25-Nov-2024 DEFINITION QPI Curve Test I0500.E0420.I0500.Q04122.E0430 ACCESSION BBa_I6304 VERSION BBa_I6304.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1959 /label=BBa_E0020 stem_loop 1979..2022 /label=stem_loop misc_feature 1968..2047 /label=BBa_B0010 terminator 1968..2047 /label=BBa_B0010 stem_loop 2063..2082 /label=T7 TE misc_feature 2089..2089 /label=stop polyA_site 2083..2096 /label=polya misc_feature 2056..2096 /label=BBa_B0012 terminator 2056..2096 /label=BBa_B0012 promoter 3184..3314 /label=Pbad promoter 3147..3218 /label=PC misc_feature 2105..3183 /label=AraC promoter 2105..3314 /label=BBa_I0500 misc_feature 3327..3330 /label=conserved RBS 3323..3334 /label=BBa_B0034 misc_feature 4430..4468 /label=LVA CDS 3341..4468 /label=lacI-LVA misc_feature 3341..4468 /label=BBa_C0012 misc_feature 4469..4493 /label=Help:Barcodes CDS 3341..4468 /label=BBa_C0012 stem_loop 4513..4556 /label=stem_loop misc_feature 4502..4581 /label=BBa_B0010 terminator 4502..4581 /label=BBa_B0010 stem_loop 4597..4616 /label=T7 TE misc_feature 4623..4623 /label=stop polyA_site 4617..4630 /label=polya misc_feature 4590..4630 /label=BBa_B0012 terminator 4590..4630 /label=BBa_B0012 CDS 4639..4726 /label=end of LacI coding region (inactive) misc_feature 4639..4838 /label=BBa_R0010 misc_feature 4727..4764 /label=CAP binding site promoter 4775..4780 /label=-35 promoter 4799..4804 /label=-10 misc_feature 4804..4838 /label=LacI binding site misc_feature 4811..4811 /label=start promoter 4639..4838 /label=BBa_R0010 misc_feature 4851..4854 /label=conserved RBS 4847..4858 /label=BBa_B0034 CDS 4865..5587 /label=BBa_E0030 stem_loop 5607..5650 /label=stem_loop misc_feature 5596..5675 /label=BBa_B0010 terminator 5596..5675 /label=BBa_B0010 stem_loop 5691..5710 /label=T7 TE misc_feature 5717..5717 /label=stop polyA_site 5711..5724 /label=polya misc_feature 5684..5724 /label=BBa_B0012 terminator 5684..5724 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagtaataat actagagcca ggcatcaaat 1981 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2041 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 2101 agagttatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact 2161 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa 2221 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg 2281 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga 2341 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa 2401 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca 2461 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca 2521 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc 2581 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg 2641 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc 2701 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg 2761 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc 2821 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa 2881 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa 2941 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc 3001 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt 3061 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa 3121 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag 3181 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa 3241 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt 3301 ttttttgggc tagctactag agaaagagga gaaatactag atggtgaatg tgaaaccagt 3361 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt 3421 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga 3481 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat 3541 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa 3601 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt 3661 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat 3721 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc 3781 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga 3841 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct 3901 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata 3961 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc 4021 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt 4081 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt 4141 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc 4201 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt 4261 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt 4321 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 4381 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga 4441 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga 4501 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 4561 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 4621 tgcgtttata tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 4681 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 4741 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 4801 tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagaaag aggagaaata 4861 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 4921 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 4981 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 5041 caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat 5101 gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 5161 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 5221 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 5281 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 5341 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 5401 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 5461 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 5521 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 5581 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 5641 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 5701 cgggtgggcc tttctgcgtt tata //