LOCUS BBa_I6307 5110 bp DNA linear 23-Dec-2024 DEFINITION QPI Curve Test I0500.E0420.I0500.Q04400.E0430 ACCESSION BBa_I6307 VERSION BBa_I6307.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1959 /label=BBa_E0020 stem_loop 1979..2022 /label=stem_loop misc_feature 1968..2047 /label=BBa_B0010 terminator 1968..2047 /label=BBa_B0010 stem_loop 2063..2082 /label=T7 TE misc_feature 2089..2089 /label=stop polyA_site 2083..2096 /label=polya misc_feature 2056..2096 /label=BBa_B0012 terminator 2056..2096 /label=BBa_B0012 promoter 3184..3314 /label=Pbad promoter 3147..3218 /label=PC misc_feature 2105..3183 /label=AraC promoter 2105..3314 /label=BBa_I0500 misc_feature 3327..3330 /label=conserved RBS 3323..3334 /label=BBa_B0034 CDS 3344..3960 /label=tetR misc_feature 3961..3994 /label=SsrA misc_feature 4001..4025 /label=Help:Barcodes CDS 3341..4000 /label=BBa_C0040 stem_loop 4045..4088 /label=stem_loop misc_feature 4034..4113 /label=BBa_B0010 terminator 4034..4113 /label=BBa_B0010 stem_loop 4129..4148 /label=T7 TE misc_feature 4155..4155 /label=stop polyA_site 4149..4162 /label=polya misc_feature 4122..4162 /label=BBa_B0012 terminator 4122..4162 /label=BBa_B0012 misc_feature 4171..4189 /label=TetR 1 misc_feature 4171..4224 /label=BBa_R0040 promoter 4190..4195 /label=-35 misc_feature 4196..4214 /label=TetR 2 promoter 4213..4218 /label=-10 promoter 4171..4224 /label=BBa_R0040 misc_feature 4237..4240 /label=conserved RBS 4233..4244 /label=BBa_B0034 CDS 4251..4973 /label=BBa_E0030 stem_loop 4993..5036 /label=stem_loop misc_feature 4982..5061 /label=BBa_B0010 terminator 4982..5061 /label=BBa_B0010 stem_loop 5077..5096 /label=T7 TE misc_feature 5103..5103 /label=stop polyA_site 5097..5110 /label=polya misc_feature 5070..5110 /label=BBa_B0012 terminator 5070..5110 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagtaataat actagagcca ggcatcaaat 1981 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2041 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 2101 agagttatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact 2161 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa 2221 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg 2281 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga 2341 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa 2401 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca 2461 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca 2521 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc 2581 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg 2641 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc 2701 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg 2761 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc 2821 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa 2881 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa 2941 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc 3001 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt 3061 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa 3121 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag 3181 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa 3241 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt 3301 ttttttgggc tagctactag agaaagagga gaaatactag atgtccagat tagataaaag 3361 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac 3421 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa 3481 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt 3541 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag 3601 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga 3661 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact 3721 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga 3781 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc 3841 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt 3901 attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc 3961 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag 4021 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 4081 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 4141 ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct 4201 atcagtgata gagatactga gcactactag agaaagagga gaaatactag atggtgagca 4261 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 4321 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 4381 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 4441 ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact 4501 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 4561 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 4621 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 4681 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 4741 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 4801 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct 4861 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 4921 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa taatactaga 4981 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5041 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 5101 tgcgtttata //