LOCUS BBa_I6319 5111 bp DNA linear 26-Dec-2024 DEFINITION QPI Curve Test I0500.E0420.I0500.Q04520.E0430 ACCESSION BBa_I6319 VERSION BBa_I6319.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1959 /label=BBa_E0020 stem_loop 1979..2022 /label=stem_loop misc_feature 1968..2047 /label=BBa_B0010 terminator 1968..2047 /label=BBa_B0010 stem_loop 2063..2082 /label=T7 TE misc_feature 2089..2089 /label=stop polyA_site 2083..2096 /label=polya misc_feature 2056..2096 /label=BBa_B0012 terminator 2056..2096 /label=BBa_B0012 promoter 3184..3314 /label=Pbad promoter 3147..3218 /label=PC misc_feature 2105..3183 /label=AraC promoter 2105..3314 /label=BBa_I0500 misc_feature 3327..3330 /label=conserved RBS 3323..3334 /label=BBa_B0034 CDS 3341..4009 /label=cI 434 misc_feature 3971..4009 /label=LVA misc_feature 3341..4009 /label=BBa_C0052 misc_feature 4010..4034 /label=Help:Barcodes CDS 3341..4009 /label=BBa_C0052 stem_loop 4054..4097 /label=stem_loop misc_feature 4043..4122 /label=BBa_B0010 terminator 4043..4122 /label=BBa_B0010 stem_loop 4138..4157 /label=T7 TE misc_feature 4164..4164 /label=stop polyA_site 4158..4171 /label=polya misc_feature 4131..4171 /label=BBa_B0012 terminator 4131..4171 /label=BBa_B0012 misc_feature 4180..4222 /label=OR2 misc_feature 4187..4200 /label=OR2 misc_feature 4209..4222 /label=OR1 promoter 4180..4185 /label=-35 promoter 4203..4208 /label=-10 promoter 4181..4186 /label=-35 misc_feature 4214..4216 /label=start misc_feature 4180..4225 /label=BBa_R0052 promoter 4180..4225 /label=BBa_R0052 misc_feature 4238..4241 /label=conserved RBS 4234..4245 /label=BBa_B0034 CDS 4252..4974 /label=BBa_E0030 stem_loop 4994..5037 /label=stem_loop misc_feature 4983..5062 /label=BBa_B0010 terminator 4983..5062 /label=BBa_B0010 stem_loop 5078..5097 /label=T7 TE misc_feature 5104..5104 /label=stop polyA_site 5098..5111 /label=polya misc_feature 5071..5111 /label=BBa_B0012 terminator 5071..5111 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagtaataat actagagcca ggcatcaaat 1981 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2041 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 2101 agagttatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact 2161 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa 2221 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg 2281 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga 2341 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa 2401 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca 2461 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca 2521 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc 2581 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg 2641 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc 2701 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg 2761 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc 2821 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa 2881 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa 2941 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc 3001 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt 3061 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa 3121 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag 3181 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa 3241 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt 3301 ttttttgggc tagctactag agaaagagga gaaatactag atgagtattt cttccagggt 3361 aaaaagcaaa agaatccagc ttggacttaa ccaggctgaa cttgctcaaa aggtggggac 3421 tacccagcag tctatagagc agctcgaaaa cggtaaaact aagcgaccac gctttttacc 3481 agaacttgcg tcagctcttg gcgtaagtgt tgactggctg ctcaatggca cctctgattc 3541 gaatgttaga tttgttgggc acgttgagcc caaagggaaa tatccattga ttagcatggt 3601 tagagctggt tcgtggtgtg aagcttgtga accctacgat atcaaggaca ttgatgaatg 3661 gtatgacagt gacgttaact tattaggcaa tggattctgg ctgaaggttg aaggtgattc 3721 catgacctca cctgtaggtc aaagcatccc tgaaggtcat atggtgttag tagatactgg 3781 acgggagcca gtgaatggaa gccttgttgt agccaaactg actgacgcga acgaagcaac 3841 attcaagaaa ctggtcatag atggcggtca gaagtacctg aaaggcctga atccttcatg 3901 gcctatgact cctatcaacg gaaactgcaa gattatcggt gttgtcgtgg aagcgagggt 3961 aaaattcgta gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 4021 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 4081 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 4141 tcaccttcgg gtgggccttt ctgcgtttat atactagagt tgacaaacaa gatacattgt 4201 atgaaaatac aagaaagttt gttgatacta gagaaagagg agaaatacta gatggtgagc 4261 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 4321 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 4381 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 4441 accttcggct acggcctgca atgcttcgcc cgctaccccg accacatgaa gctgcacgac 4501 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 4561 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 4621 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 4681 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 4741 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 4801 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 4861 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 4921 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag 4981 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 5041 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 5101 ctgcgtttat a //