LOCUS       BBa_I6413                587 bp    DNA     linear       25-Apr-2024
DEFINITION  Promoter high output protein generator
ACCESSION   BBa_I6413
VERSION     BBa_I6413.1  
FEATURES             Location/Qualifiers
     promoter        10..15
                     /label=-35
     promoter        33..38
                     /label=-10
     misc_feature    20..33
                     /label=Zif23
     misc_feature    1..9
                     /label=consensus
     misc_feature    45..45
                     /label=(+1)
     promoter        1..45
                     /label=BBa_R2000
     misc_feature    58..61
                     /label=conserved
     RBS             54..65
                     /label=BBa_B0034
     misc_feature    72..86
                     /label=lacZ gene fragment
     CDS             72..425
                     /label=lacZ alpha
     promoter        97..106
                     /label=T3 promoter
     promoter        244..262
                     /label=T7 promoter
     misc_feature    149..149
                     /label=C
     misc_feature    152..152
                     /label=T
     misc_feature    158..158
                     /label=A
     misc_feature    179..179
                     /label=C
     misc_feature    182..182
                     /label=G
     misc_feature    270..419
                     /label=lacZ gene fragment
     misc_feature    419..419
                     /label=inserted T
     misc_feature    426..450
                     /label=barcode
     misc_feature    72..450
                     /label=BBa_E0035
     stem_loop       470..513
                     /label=stem_loop
     misc_feature    459..538
                     /label=BBa_B0010
     terminator      459..538
                     /label=BBa_B0010
     stem_loop       554..573
                     /label=T7 TE
     misc_feature    580..580
                     /label=stop
     polyA_site      574..587
                     /label=polya
     misc_feature    547..587
                     /label=BBa_B0012
     terminator      547..587
                     /label=BBa_B0012
     terminator      459..587
                     /label=BBa_B0015
     misc_feature    54..587
                     /label=BBa_E0435
ORIGIN
        1 agtttattct tgacatggtc ccacgcgcgt gggatactac gtcagtacta gagaaagagg
       61 agaaatacta gatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
      121 aaagctggag ctccaccgcg gtggcggcag cactagagct agtggatccc ccgggctgta
      181 gaaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt
      241 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
      301 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      361 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatt
      421 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
      481 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      541 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//