LOCUS BBa_I6413 587 bp DNA linear 22-Dec-2024 DEFINITION Promoter high output protein generator ACCESSION BBa_I6413 VERSION BBa_I6413.1 FEATURES Location/Qualifiers promoter 10..15 /label=-35 promoter 33..38 /label=-10 misc_feature 20..33 /label=Zif23 misc_feature 1..9 /label=consensus misc_feature 45..45 /label=(+1) promoter 1..45 /label=BBa_R2000 misc_feature 58..61 /label=conserved RBS 54..65 /label=BBa_B0034 misc_feature 72..86 /label=lacZ gene fragment CDS 72..425 /label=lacZ alpha promoter 97..106 /label=T3 promoter promoter 244..262 /label=T7 promoter misc_feature 149..149 /label=C misc_feature 152..152 /label=T misc_feature 158..158 /label=A misc_feature 179..179 /label=C misc_feature 182..182 /label=G misc_feature 270..419 /label=lacZ gene fragment misc_feature 419..419 /label=inserted T misc_feature 426..450 /label=barcode misc_feature 72..450 /label=BBa_E0035 stem_loop 470..513 /label=stem_loop misc_feature 459..538 /label=BBa_B0010 terminator 459..538 /label=BBa_B0010 stem_loop 554..573 /label=T7 TE misc_feature 580..580 /label=stop polyA_site 574..587 /label=polya misc_feature 547..587 /label=BBa_B0012 terminator 547..587 /label=BBa_B0012 terminator 459..587 /label=BBa_B0015 misc_feature 54..587 /label=BBa_E0435 ORIGIN 1 agtttattct tgacatggtc ccacgcgcgt gggatactac gtcagtacta gagaaagagg 61 agaaatacta gatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca 121 aaagctggag ctccaccgcg gtggcggcag cactagagct agtggatccc ccgggctgta 181 gaaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt 241 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact 301 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 361 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatt 421 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa 481 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 541 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //