BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_I711001
1
BBa_I711001
generates the tyrosinase for melanin biosynthesis
2007-05-25T11:00:00Z
2015-08-31T04:07:45Z
Registry promoter, RBS, and terminator. this is the generator for tyrosinase gene
Constitutive promoter
false
false
_158_
0
1496
9
Not in stock
false
no design considerations
false
Ron Bauerle
component1933854
1
BBa_B0030
component1933852
1
BBa_J23119
component1933862
1
BBa_B0011
component1933858
1
BBa_B0012
component1933857
1
BBa_I711000
annotation1933862
1
BBa_B0011
range1933862
1
947
992
annotation1933857
1
BBa_I711000
range1933857
1
65
889
annotation1933854
1
BBa_B0030
range1933854
1
44
58
annotation1933858
1
BBa_B0012
range1933858
1
898
938
annotation1933852
1
BBa_J23119
range1933852
1
1
35
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_I711000
1
BBa_I711000
tyrosinase (mel) gene
2007-05-25T11:00:00Z
2015-08-31T04:07:45Z
s. antibioticus
The nucleotide sequence of the tyrosinase gene from Streptomyces
antibioticus and characterization of the gene product
false
false
_158_
0
1496
9
Not in stock
false
Only one part of 2 needed for production of melanin
false
Ron Bauerle
annotation1933732
1
our start site
range1933732
1
1
4
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_I711000_sequence
1
atgaccgtccgcaagaaccaggcgtccctgaccgccgaggagaagcgccgcttcgtcgccgccctgctcgaactcaagcgcaccggccgctacgacgccttcgtcaccacgcacaacgcgttcatcctgggcgacaccgacaacggcgagcgcaccggccaccgttcgccgtccttcctgccctggcaccgcagatttctgctggagttcgagcgggcgctccagtcggtggacgcgtcggtggcgctgccgtactgggactggtccgccgaccggtccacccggtcctcgctgtgggcgccggacttcctcggcggcaccgggcgcagccgggacggccaggtgatggacgggccgttcgccgcgtcggccggcaactggccgatcaatgtgcgggtggacggccgtacgttcctgcggcgggcgctcggcgcgggcgtgagcgaactgcccacgcgtgccgaggtcgactcggtgctggcgatggcgacgtacgacatggcgccctggaacagcggctccgacggcttccgcaaccatctcgaagggtggcgcggggtcaatctgcacaaccgggtgcatgtctgggtcggcggccagatggcgaccggggtctcccccaacgacccggtgttctggctgcaccacgcctacatcgacaagctgtgggccgagtggcagcggcggcacccctcgtccccgtatctgccgggcggcggcacgccgaacgtcgtcgacctcaacgagacgatgaagccgtggaacgacaccaccccggcggccctgctggaccacacccggcactacaccttcgacgtctaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I711001_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgaccgtccgcaagaaccaggcgtccctgaccgccgaggagaagcgccgcttcgtcgccgccctgctcgaactcaagcgcaccggccgctacgacgccttcgtcaccacgcacaacgcgttcatcctgggcgacaccgacaacggcgagcgcaccggccaccgttcgccgtccttcctgccctggcaccgcagatttctgctggagttcgagcgggcgctccagtcggtggacgcgtcggtggcgctgccgtactgggactggtccgccgaccggtccacccggtcctcgctgtgggcgccggacttcctcggcggcaccgggcgcagccgggacggccaggtgatggacgggccgttcgccgcgtcggccggcaactggccgatcaatgtgcgggtggacggccgtacgttcctgcggcgggcgctcggcgcgggcgtgagcgaactgcccacgcgtgccgaggtcgactcggtgctggcgatggcgacgtacgacatggcgccctggaacagcggctccgacggcttccgcaaccatctcgaagggtggcgcggggtcaatctgcacaaccgggtgcatgtctgggtcggcggccagatggcgaccggggtctcccccaacgacccggtgttctggctgcaccacgcctacatcgacaagctgtgggccgagtggcagcggcggcacccctcgtccccgtatctgccgggcggcggcacgccgaacgtcgtcgacctcaacgagacgatgaagccgtggaacgacaccaccccggcggccctgctggaccacacccggcactacaccttcgacgtctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z