BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_R0084
1
OmpR
Promoter (OmpR, positive)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
NC_000913 E. coli K12
Released HQ 2013
Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
false
false
_1_
0
24
7
In stock
false
The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
true
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
annotation301308
1
F1 OmpR
range301308
1
11
28
annotation301318
1
-10
range301318
1
88
93
annotation301313
1
F3 OmpR
range301313
1
51
68
annotation301317
1
-35
range301317
1
73
78
annotation301309
1
F2 OmpR
range301309
1
31
48
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_I711005
1
BBa_I711005
Melanogenesis with red light trigger
2007-06-04T11:00:00Z
2015-08-31T04:07:45Z
S. antibiotica
This part is triggered by BBa_1711006 (which detects red light) and produces tyrosinase and ORF438, two proteins known to be important for melanogenesis.
false
false
_158_
0
1498
9
Not in stock
false
The promoter region may have to be modified to be activated by BBa_I711006.
false
VGEM 2007
component1934544
1
BBa_B0030
component1934551
1
BBa_B0011
component1934547
1
BBa_B0012
component1934542
1
BBa_R0084
component1934546
1
BBa_I711004
annotation1934547
1
BBa_B0012
range1934547
1
1441
1481
annotation1934544
1
BBa_B0030
range1934544
1
117
131
annotation1934551
1
BBa_B0011
range1934551
1
1490
1535
annotation1934546
1
BBa_I711004
range1934546
1
138
1432
annotation1934542
1
BBa_R0084
range1934542
1
1
108
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_I711004
1
BBa_I711004
Generates tyrosinase (mel) and ORF438 for melanogenesis
2007-06-04T11:00:00Z
2015-08-31T04:07:45Z
S. antibiotica
The nucleotide sequence of the tyrosinase gene (mel) and an upstream ORF from S.antibioticus. From the paper titled Melanin Production in Escherichia Coli from a Cloned Tyrosinase Gene by Bernan,V., Filpula,D., Herber,W., Bibb,M. and Katz,E.
false
false
_158_
0
1498
9
Not in stock
false
None
false
VGEM 2007
BBa_I711005_sequence
1
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgcatactagagattaaagaggagaaatactagatgccggaactcacccgtcgtcgcgcgctcggcgccgcagccgtcgtcgccgccggtgtcccgctggtcgcccttcccgccgcccgcgcggacgatcgggggcaccacacccccgaggtccccgggaacccggccgcgtccggcgcccccgccgccttcgacgagatctacaagggccgccggatacagggccggacggtcaccgacggcgggggccaccacggcggcggtcacggcggtgacggtcacggcggcggccatcacggcggcggttacgccgtgttcgtggacggcgtcgaactgcatgtgatgcgcaacgccgacggctcgtggatcagcgtcgtcagccactacgagccggtggacaccccgcgcgccgcggcccgcgctgcggtcgacgagctccagggcgcccggctcctccccttcccctccaactgaccttctcccccgcacttttggagcacccgcacatgaccgtccgcaagaaccaggcgtccctgaccgccgaggagaagcgccgcttcgtcgccgccctgctcgaactcaagcgcaccggccgctacgacgccttcgtcaccacgcacaacgcgttcatcctgggcgacaccgacaacggcgagcgcaccggccaccgttcgccgtccttcctgccctggcaccgcagatttctgctggagttcggcgggcgctccagtcggtggacgcgtcggtggcgctgccgtactgggactggtccgcgaccggtccacccggtcctcgctgtgggcgccggacttcctcggcggcaccgggcgcagccgggacggccaggtgatggacgggccgttcgccgcgtcggccggcaactggccgatcaatgtgcgggtggacggccgtacgttcctgcggcgggcgctcggcgcgggcgtgagcgaactgcccacgcgtgccgaggtcgactcggtgctggcgatggcgacgtacgacatggcgccctggaacagcggctccgacggcttccgcaaccatctcgaagggtggccggggtcaatctgcacaaccgggtgcatgtctgggtcggcggccagatggcgaccggggtctcccccaacgacccggtgttctggctgcaccacgcctacatcgacaagctgtgggccgagtggcagcggcggcacccctcgtccccgtatctgccgggcggcggcacgccgaacgtcgtcgacctcaacgagacgatgaagccgtggaacgacaccaccccggcggccctgctggaccacacccggcactacaccttcgacgtctgatcatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_I711004_sequence
1
atgccggaactcacccgtcgtcgcgcgctcggcgccgcagccgtcgtcgccgccggtgtcccgctggtcgcccttcccgccgcccgcgcggacgatcgggggcaccacacccccgaggtccccgggaacccggccgcgtccggcgcccccgccgccttcgacgagatctacaagggccgccggatacagggccggacggtcaccgacggcgggggccaccacggcggcggtcacggcggtgacggtcacggcggcggccatcacggcggcggttacgccgtgttcgtggacggcgtcgaactgcatgtgatgcgcaacgccgacggctcgtggatcagcgtcgtcagccactacgagccggtggacaccccgcgcgccgcggcccgcgctgcggtcgacgagctccagggcgcccggctcctccccttcccctccaactgaccttctcccccgcacttttggagcacccgcacatgaccgtccgcaagaaccaggcgtccctgaccgccgaggagaagcgccgcttcgtcgccgccctgctcgaactcaagcgcaccggccgctacgacgccttcgtcaccacgcacaacgcgttcatcctgggcgacaccgacaacggcgagcgcaccggccaccgttcgccgtccttcctgccctggcaccgcagatttctgctggagttcggcgggcgctccagtcggtggacgcgtcggtggcgctgccgtactgggactggtccgcgaccggtccacccggtcctcgctgtgggcgccggacttcctcggcggcaccgggcgcagccgggacggccaggtgatggacgggccgttcgccgcgtcggccggcaactggccgatcaatgtgcgggtggacggccgtacgttcctgcggcgggcgctcggcgcgggcgtgagcgaactgcccacgcgtgccgaggtcgactcggtgctggcgatggcgacgtacgacatggcgccctggaacagcggctccgacggcttccgcaaccatctcgaagggtggccggggtcaatctgcacaaccgggtgcatgtctgggtcggcggccagatggcgaccggggtctcccccaacgacccggtgttctggctgcaccacgcctacatcgacaagctgtgggccgagtggcagcggcggcacccctcgtccccgtatctgccgggcggcggcacgccgaacgtcgtcgacctcaacgagacgatgaagccgtggaacgacaccaccccggcggccctgctggaccacacccggcactacaccttcgacgtctgatca
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_R0084_sequence
1
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgca
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z