LOCUS BBa_I711019 5362 bp DNA linear 25-Nov-2024 DEFINITION Butyryl-CoA -> Butanol (adhe, adhe1) ACCESSION BBa_I711019 VERSION BBa_I711019.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 CDS 65..2653 /label=BBa_I711008 RBS 2662..2676 /label=RBS-1\Strong RBS 2669..2673 /label=RBS misc_feature 2662..2676 /label=BBa_B0030 RBS 2662..2676 /label=BBa_B0030 CDS 2683..5259 /label=BBa_I711009 stem_loop 5275..5294 /label=T7 TE misc_feature 5301..5301 /label=stop polyA_site 5295..5308 /label=polya misc_feature 5268..5308 /label=BBa_B0012 terminator 5268..5308 /label=BBa_B0012 stem_loop 5329..5351 /label=stem_loop misc_feature 5317..5362 /label=BBa_B0011 terminator 5317..5362 /label=BBa_B0011 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagattaaag aggagaaata 61 ctagatgaaa gtcacaacag taaaggaatt agatgaaaaa ctcaaggtaa ttaaagaagc 121 tcaaaaaaaa ttctcttgtt actcgcaaga aatggttgat gaaatcttta gaaatgcagc 181 aatggcagca atcgacgcaa ggatagagct agcaaaagca gctgttttgg aaaccggtat 241 gggcttagtt gaagacaagg ttataaaaaa tcattttgca ggcgaataca tctataacaa 301 atataaggat gaaaaaacct gcggtataat tgaacgaaat gaaccctacg gaattacaaa 361 aatagcagaa cctataggag ttgtagctgc tataatccct gtaacaaacc ccacatcaac 421 aacaatattt aaatccttaa tatcccttaa aactagaaat ggaattttct tttcgcctca 481 cccaagggca aaaaaatcca caatactagc agctaaaaca atacttgatg cagccgttaa 541 gagtggtgcc ccggaaaata taataggttg gatagatgaa ccttcaattg aactaactca 601 atatttaatg caaaaagcag atataaccct tgcaactggt ggtccctcac tggttaaatc 661 tgcttattct tccggaaaac cagcaatagg tgttggtccg ggtaacaccc cagtaataat 721 tgatgaatct gctcatataa aaatggcagt aagttcaatt atattatcca aaacctatga 781 taatggtgtt atatgtgctt ctgaacaatc tgtaatagtc ttaaaatcca tatataacaa 841 ggtaaaagat gagttccaag aaagaggagc ttatataata aagaaaaacg aattggataa 901 agtccgtgaa gtgattttta aagatggatc cgtaaaccct aaaatagtcg gacagtcagc 961 ttatactata gcagctatgg ctggcataaa agtacctaaa accacaagaa tattaatagg 1021 agaagttacc tccttaggtg aagaagaacc ttttgcccac gaaaaactat ctcctgtttt 1081 ggctatgtat gaggctgaca attttgatga tgctttaaaa aaagcagtaa ctctaataaa 1141 cttaggaggc ctcggccata cctcaggaat atatgcagat gaaataaaag cacgagataa 1201 aatagataga tttagtagtg ccatgaaaac cgtaagaacc tttgtaaata tcccaacctc 1261 acaaggtgca agtggagatc tatataattt tagaatacca ccttctttca cgcttggctg 1321 cggattttgg ggaggaaatt ctgtttccga gaatgttggt ccaaaacatc ttttgaatat 1381 taaaaccgta gctgaaagga gagaaaacat gctttggttt agagttccac ataaagtata 1441 ttttaagttc ggttgtcttc aatttgcttt aaaagattta aaagatctaa agaaaaaaag 1501 agcctttata gttactgata gtgaccccta taatttaaac tatgttgatt caataataaa 1561 aatacttgag cacctagata ttgattttaa agtatttaat aaggttggaa gagaagctga 1621 tcttaaaacc ataaaaaaag caactgaaga aatgtcctcc tttatgccag acactataat 1681 agctttaggt ggtacccctg aaatgagctc tgcaaagcta atgtgggtac tatatgaaca 1741 tccagaagta aaatttgaag atcttgcaat aaaatttatg gacataagaa agagaatata 1801 tactttccca aaactcggta aaaaggctat gttagttgca attacaactt ctgctggttc 1861 cggttctgag gttactcctt ttgctttagt aactgacaat aacactggaa ataagtacat 1921 gttagcagat tatgaaatga caccaaatat ggcaattgta gatgcagaac ttatgatgaa 1981 aatgccaaag ggattaaccg cttattcagg tatagatgca ctggtaaata gtatagaagc 2041 atacacatcc gtatatgctt cagaatacac aaacggacta gcactagagg caatacgatt 2101 aatatttaaa tatttgcctg aggcttacaa aaacggaaga accaatgaaa aagcaagaga 2161 gaaaatggct cacgcttcaa ctatggcagg tatggcatcc gctaatgcat ttctaggtct 2221 atgtcattcc atggcaataa aattaagttc agaacacaat attcctagtg gcattgccaa 2281 tgcattacta atagaagaag taataaaatt taacgcagtt gataatcctg taaaacaagc 2341 cccttgccca caatataagt atccaaacac catatttaga tatgctcgaa ttgcagatta 2401 tataaagctt ggaggaaata ctgatgagga aaaggtagat ctcttaatta acaaaataca 2461 tgaactaaaa aaagctttaa atataccaac ttcaataaag gatgcaggtg ttttggagga 2521 aaacttctat tcctcccttg atagaatatc tgaacttgca ctagatgatc aatgcacagg 2581 cgctaatcct agatttcctc ttacaagtga gataaaagaa atgtatataa attgttttaa 2641 aaaacaacct taatactaga gattaaagag gagaaatact agatgaaagt tacaaatcaa 2701 aaagaactaa aacaaaagct aaatgaattg agagaagcgc aaaagaagtt tgcaacctat 2761 actcaagagc aagttgataa aatttttaaa caatgtgcca tagccgcagc taaagaaaga 2821 ataaacttag ctaaattagc agtagaagaa acaggaatag gtcttgtaga agataaaatt 2881 ataaaaaatc attttgcagc agaatatata tacaataaat ataaaaatga aaaaacttgt 2941 ggcataatag accatgacga ttctttaggc ataacaaagg ttgctgaacc aattggaatt 3001 gttgcagcca tagttcctac tactaatcca acttccacag caattttcaa atcattaatt 3061 tctttaaaaa caagaaacgc aatattcttt tcaccacatc cacgtgcaaa aaaatctaca 3121 attgctgccg caaaattaat tttagatgca gctgttaaag caggagcacc taaaaatata 3181 ataggctgga tagatgagcc atcaatagaa ctttctcaag atttgatgag tgaagctgat 3241 ataatattag caacaggagg tccttcaatg gttaaagcgg cctattcatc tggaaaacct 3301 gcaattggtg ttggagcagg aaatacacca gcaataatag atgagagtgc agatatagat 3361 atggcagtaa gctccataat tttatcaaag acttatgaca atggagtaat atgcgcttct 3421 gaacaatcaa tattagttat gaatccaata tacgaaaaag ttaaagagga atttgtaaaa 3481 cgaggatcat atatactcaa tcaaaatgaa atagctaaaa taaaagaaac tatgtttaaa 3541 aatggagcta ttaatgctga catagttgga aaatctgctt atataattgc taaaatggca 3601 ggaattgaag ttcctcaaac tacaaagata cttataggcg aagtacaatc tgttgaaaaa 3661 agcgagctgt tctcacatga aaaactatca ccagtacttg caatgtataa agttaaggat 3721 tttgatgaag ctctaaaaaa ggcacaaagg ctaatagaat taggtggaag tggacacacg 3781 tcatctttat atatagattc acaaaacaat aaggataaag ttaaagaatt tggattagca 3841 atgaaaactt caaggacatt tattaacatg ccttcttcac agggagcaag cggagattta 3901 tacaattttg cgatagcacc atcatttact cttggatgcg gcacttgggg aggaaactct 3961 gtatcgcaaa atgtagagcc taaacattta ttaaatatta aaagtgttgc tgaaagaagg 4021 gaaaatatgc tttggtttaa agtgccacaa aaaatatatt ttaaatatgg atgtcttaga 4081 tttgcattaa aagaattaaa agatatgaat aagaaaagag cctttatagt aacagataaa 4141 gatcttttta aacttggata tgttaataaa ataacaaagg tactagatga gatagatatt 4201 aaatacagta tatttacaga tattaaatct gatccaacta ttgattcagt aaaaaaaggt 4261 gctaaagaaa tgcttaactt tgaacctgat actataatct ctattggtgg tggatcgcca 4321 atggatgcag caaaggttat gcacttgtta tatgaatatc cagaagcaga aattgaaaat 4381 ctagctataa actttatgga tataagaaag agaatatgca atttccctaa attaggtaca 4441 aaggcgattt cagtagctat tcctacaact gctggtaccg gttcagaggc aacacctttt 4501 gcagttataa ctaatgatga aacaggaatg aaataccctt taacttctta tgaattgacc 4561 ccaaacatgg caataataga tactgaatta atgttaaata tgcctagaaa attaacagca 4621 gcaactggaa tagatgcatt agttcatgct atagaagcat atgtttcggt tatggctacg 4681 gattatactg atgaattagc cttaagagca ataaaaatga tatttaaata tttgcctaga 4741 gcctataaaa atgggactaa cgacattgaa gcaagagaaa aaatggcaca tgcctctaat 4801 attgcgggga tggcatttgc aaatgctttc ttaggtgtat gccattcaat ggctcataaa 4861 cttggggcaa tgcatcacgt tccacatgga attgcttgtg ctgtattaat agaagaagtt 4921 attaaatata acgctacaga ctgtccaaca aagcaaacag cattccctca atataaatct 4981 cctaatgcta agagaaaata tgctgaaatt gcagagtatt tgaatttaaa gggtactagc 5041 gataccgaaa aggtaacagc cttaatagaa gctatttcaa agttaaagat agatttgagt 5101 attccacaaa atataagtgc cgctggaata aataaaaaag atttttataa tacgctagat 5161 aaaatgtcag agcttgcttt tgatgaccaa tgtacaacag ctaatcctag gtatccactt 5221 ataagtgaac ttaaggatat ctatataaaa tcattttaat actagagtca cactggctca 5281 ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa gccagattat 5341 taatccggct tttttattat tt //