BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_I711009 1 BBa_I711009 adhe from Clostridium acetobutylicum ATCC 824 2007-07-11T11:00:00Z 2015-08-31T04:07:45Z Clostridium acetobutylicum ATCC 824 This sequence codes for an aldehyde dehydrogenase that is responsible for making butyraldehyde from butyryl-CoA. false false _158_ 0 1498 9 Not in stock false None false Ranjan Khan BBa_I711008 1 BBa_I711008 aad/adhE from Clostridium acetobutylicum ATCC 824 2007-07-11T11:00:00Z 2015-08-31T04:07:45Z Clostridium acetobutylicum ATCC 824 This sequence codes for an aldehyde dehydrogenase required to take butyl-CoA to butyraldehyde. false false _158_ 0 1498 9 Not in stock false None false Ranjan Khan BBa_I711019 1 BBa_I711019 Butyryl-CoA -> Butanol (adhe, adhe1) 2007-07-15T11:00:00Z 2015-08-31T04:07:45Z Clostridium acetobutylicum ATCC 824 This part constituitvely translates the adhe and adhe1 genes. The enzymes produced create Beta-butylaldehyde from butyryl-CoA and create butanol from Beta-butylaldehyde. true false _158_ 0 1498 9 Discontinued false None. false Ranjan Khan component1938376 1 BBa_I711009 component1938368 1 BBa_J23119 component1938374 1 BBa_B0030 component1938370 1 BBa_B0030 component1938372 1 BBa_I711008 component1938381 1 BBa_B0011 component1938377 1 BBa_B0012 annotation1938377 1 BBa_B0012 range1938377 1 5268 5308 annotation1938374 1 BBa_B0030 range1938374 1 2662 2676 annotation1938370 1 BBa_B0030 range1938370 1 44 58 annotation1938381 1 BBa_B0011 range1938381 1 5317 5362 annotation1938368 1 BBa_J23119 range1938368 1 1 35 annotation1938372 1 BBa_I711008 range1938372 1 65 2653 annotation1938376 1 BBa_I711009 range1938376 1 2683 5259 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation1683 1 stem_loop range1683 1 13 35 annotation7019 1 BBa_B0011 range7019 1 1 46 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_I711008_sequence 1 atgaaagtgaccacggttaaagagctggatgaaaagctgaaagtaatcaaagaggcacagaagaaatttagctgctatagccaggaaatggttgatgagatctttcgtaatgcggctatggcagcgatcgatgctcgtattgaactggcgaaagctgcggttctggagacgggtatgggcctggtggaagacaaagtaatcaaaaaccacttcgctggtgaatatatctataataagtacaaagatgagaaaacttgcggcattatcgaacgcaatgaaccgtacggcatcaccaaaattgcagaaccgattggtgtcgttgcagcaatcatcccggtcaccaacccgacttctaccaccattttcaaaagcctgatcagcctgaaaacccgcaacggtatcttcttcagccctcacccacgtgctaaaaagtccacgatcctggccgcaaaaaccatcctggacgcggcggttaaatccggtgctccggaaaacattatcggctggatcgatgaaccgtccattgagctgacccagtatctgatgcaaaaagcagatattaccctggctaccggcggtccgtctctggttaaatctgcatactccagcggcaaaccagctatcggcgtcggtccgggcaacactccggtgattatcgacgaatccgcacacattaagatggcagtttcctccatcattctgtctaagacctacgataacggtgttatttgtgcatccgagcagtccgttatcgttctgaaatctatctacaacaaagtcaaagatgagttccaggaacgtggtgcgtacatcattaagaaaaatgaactggataaagttcgtgaagttatctttaaggatggcagcgtcaacccgaaaatcgtgggtcagagcgcatacacgatcgctgcgatggctggcattaaagttccgaaaaccacccgtattctgatcggtgaagttacttctctgggcgaagaagagccgttcgctcacgaaaagctgagcccggtgctggccatgtacgaagcagacaactttgacgatgcgctgaaaaaggccgtgacgctgatcaacctgggtggtctgggtcacacttctggcatctatgctgacgaaattaaagcacgcgacaaaatcgaccgtttttctagcgctatgaaaaccgttcgtactttcgtcaacatcccaacttctcagggtgccagcggtgacctgtacaactttcgtatcccaccgtctttcactctgggctgtggtttctggggtggcaactctgtttctgaaaacgttggtcctaaacacctgctgaacatcaaaaccgtcgccgaacgccgcgaaaacatgctgtggtttcgtgttcctcataaggtttatttcaaattcggctgtctgcaattcgcgctgaaggacctgaaagacctgaaaaagaaacgtgcgtttatcgttaccgattctgacccgtacaacctgaactatgttgacagcatcattaaaatcctggaacacctggacattgacttcaaagtttttaacaaagtaggtcgcgaggcggacctgaaaaccattaaaaaggcaaccgaagaaatgtcttccttcatgccggacactatcattgctctgggtggcaccccggaaatgtcctctgctaaactgatgtgggttctgtacgaacacccggaggtgaagttcgaggatctggctatcaaattcatggacattcgtaaacgcatctacacgttccctaaactgggtaagaaagcgatgctggtcgctatcaccaccagcgccggttccggcagcgaagtcaccccgtttgctctggttaccgataacaacactggtaacaaatacatgctggcagattacgaaatgactccgaacatggctatcgttgacgcggaactgatgatgaaaatgccgaaaggtctgaccgcatacagcggtatcgacgcgctggtaaactctatcgaggcctatacctccgtttacgcatctgaatacaccaacggtctggcgctggaagcgatccgcctgattttcaagtacctgccagaagcatacaaaaatggtcgcactaacgaaaaagcgcgcgaaaagatggcacatgcgagcaccatggcgggtatggcctctgctaacgcgttcctgggcctgtgccactccatggctattaaactgtctagcgaacacaatattccgtctggcatcgcgaacgctctgctgatcgaagaggtaattaaattcaacgcggttgacaacccagttaaacaggcaccgtgtccacagtataagtaccctaatactatcttccgctatgcgcgtatcgccgactacatcaaactgggtggtaataccgatgaagaaaaagtcgatctgctgattaacaaaatccatgaactgaaaaaggctctgaacatccctacctctattaaagacgctggtgttctggaagaaaacttctactcctccctggatcgtatcagcgagctggctctggatgatcagtgtacgggcgctaacccgcgtttccctctgacctccgaaattaaggagatgtacattaactgcttcaagaaacagccg BBa_I711009_sequence 1 atgaaagtaactaaccagaaagaactgaaacagaagctgaacgaactgcgcgaagcgcagaagaaattcgccacctacactcaggaacaagtcgataagattttcaaacagtgcgcaatcgcggcagctaaagaacgtatcaacctggcgaaactggcggttgaagaaactggcatcggcctggtagaagacaaaattatcaaaaaccattttgcagcagaatacatctacaacaaatacaaaaacgagaaaacctgtggcattattgaccatgacgattccctgggtatcactaaagttgccgaaccgattggtatcgtggctgcgatcgttccgacgactaacccgactagcaccgctatctttaaaagcctgatctctctgaagactcgtaacgcgatcttctttagcccacacccgcgtgcgaagaaatccaccattgcagccgccaaactgattctggacgctgcggtaaaagcaggtgcaccgaaaaacattattggctggatcgatgagccgtctatcgaactgtcccaggacctgatgtctgaagcggacatcattctggcgactggtggtccatctatggttaaggcggcatactcttccggcaaaccggctatcggtgttggcgcgggtaacaccccggctatcattgacgaaagcgctgacatcgacatggcagtttcctccatcatcctgtctaaaacctatgacaacggcgttatttgtgcatctgagcagagcatcctggtaatgaactctatttacgaaaaagtaaaagaagaatttgttaaacgtggttcctacattctgaatcagaacgaaatcgcaaagatcaaagaaaccatgttcaaaaacggcgcgatcaatgctgatattgttggcaaatctgcgtacatcattgctaaaatggccggcattgaggttccgcagactaccaaaattctgatcggtgaagtccagtctgttgaaaaatccgagctgttctcccatgagaaactgagcccggttctggctatgtacaaagtaaaagacttcgatgaagcactgaagaaagcgcagcgcctgatcgaactgggtggcagcggtcacacttctagcctgtacatcgattcccagaataacaaagataaagttaaggaatttggcctggcaatgaaaacctctcgcacttttatcaatatgccgtcttctcaaggtgcctctggcgacctgtacaactttgcgattgctccgtccttcaccctgggctgcggtacctggggcggtaactctgtcagccagaacgtcgaaccgaaacacctgctgaacatcaaatccgttgcggaacgccgtgaaaacatgctgtggtttaaagtcccgcagaaaatctatttcaaatacggttgtctgcgttttgctctgaaagagctgaaagacatgaacaagaaacgtgccttcattgtgaccgataaagacctgttcaaactgggttatgtaaacaagatcactaaagtactggacgagatcgatatcaagtatagcatcttcaccgacattaaatccgacccgactatcgactccgtcaagaaaggcgctaaagaaatgctgaacttcgaaccggacaccatcatcagcatcggtggtggttctccgatggacgccgcaaaggtgatgcacctgctgtatgaatatccggaagctgagatcgaaaacctggcaatcaacttcatggacatccgcaaacgcatctgcaacttcccgaaactgggtaccaaagcaatctctgtggccatcccgaccactgcgggcaccggttctgaggcgacgccgttcgccgtgatcaccaacgatgaaactggtatgaaatacccgctgaccagctatgaactgaccccgaacatggccatcattgataccgaactgatgctgaatatgccgcgtaaactgactgctgctaccggtattgacgcactggtacacgcgatcgaggcgtacgtatctgtcatggctacggactacactgatgaactggccctgcgcgcaatcaaaatgatcttcaaatatctgccgcgtgcgtataaaaacggcaccaacgacatcgaagctcgcgagaaaatggcacacgcctctaatatcgcaggcatggcattcgcaaacgctttcctgggtgtttgccactctatggcacataaactgggcgccatgcaccacgtccctcacggtattgcttgcgctgttctgatcgaagaggttattaagtataacgctaccgattgtccgaccaaacagactgcatttccgcagtacaaatctccgaacgcgaaacgtaagtacgcggagatcgccgaatacctgaacctgaaaggtacttccgatactgagaaagtgaccgcgctgatcgaagcgatcagcaagctgaaaatcgacctgtccattccgcagaacatctctgcggcgggtatcaataagaaagatttctacaacaccctggataaaatgagcgaactggctttcgacgaccagtgtaccactgctaaccctcgttacccgctgattagcgaactgaaggatatctacatcaaatccttc BBa_B0030_sequence 1 attaaagaggagaaa BBa_I711019_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgaaagtcacaacagtaaaggaattagatgaaaaactcaaggtaattaaagaagctcaaaaaaaattctcttgttactcgcaagaaatggttgatgaaatctttagaaatgcagcaatggcagcaatcgacgcaaggatagagctagcaaaagcagctgttttggaaaccggtatgggcttagttgaagacaaggttataaaaaatcattttgcaggcgaatacatctataacaaatataaggatgaaaaaacctgcggtataattgaacgaaatgaaccctacggaattacaaaaatagcagaacctataggagttgtagctgctataatccctgtaacaaaccccacatcaacaacaatatttaaatccttaatatcccttaaaactagaaatggaattttcttttcgcctcacccaagggcaaaaaaatccacaatactagcagctaaaacaatacttgatgcagccgttaagagtggtgccccggaaaatataataggttggatagatgaaccttcaattgaactaactcaatatttaatgcaaaaagcagatataacccttgcaactggtggtccctcactggttaaatctgcttattcttccggaaaaccagcaataggtgttggtccgggtaacaccccagtaataattgatgaatctgctcatataaaaatggcagtaagttcaattatattatccaaaacctatgataatggtgttatatgtgcttctgaacaatctgtaatagtcttaaaatccatatataacaaggtaaaagatgagttccaagaaagaggagcttatataataaagaaaaacgaattggataaagtccgtgaagtgatttttaaagatggatccgtaaaccctaaaatagtcggacagtcagcttatactatagcagctatggctggcataaaagtacctaaaaccacaagaatattaataggagaagttacctccttaggtgaagaagaaccttttgcccacgaaaaactatctcctgttttggctatgtatgaggctgacaattttgatgatgctttaaaaaaagcagtaactctaataaacttaggaggcctcggccatacctcaggaatatatgcagatgaaataaaagcacgagataaaatagatagatttagtagtgccatgaaaaccgtaagaacctttgtaaatatcccaacctcacaaggtgcaagtggagatctatataattttagaataccaccttctttcacgcttggctgcggattttggggaggaaattctgtttccgagaatgttggtccaaaacatcttttgaatattaaaaccgtagctgaaaggagagaaaacatgctttggtttagagttccacataaagtatattttaagttcggttgtcttcaatttgctttaaaagatttaaaagatctaaagaaaaaaagagcctttatagttactgatagtgacccctataatttaaactatgttgattcaataataaaaatacttgagcacctagatattgattttaaagtatttaataaggttggaagagaagctgatcttaaaaccataaaaaaagcaactgaagaaatgtcctcctttatgccagacactataatagctttaggtggtacccctgaaatgagctctgcaaagctaatgtgggtactatatgaacatccagaagtaaaatttgaagatcttgcaataaaatttatggacataagaaagagaatatatactttcccaaaactcggtaaaaaggctatgttagttgcaattacaacttctgctggttccggttctgaggttactccttttgctttagtaactgacaataacactggaaataagtacatgttagcagattatgaaatgacaccaaatatggcaattgtagatgcagaacttatgatgaaaatgccaaagggattaaccgcttattcaggtatagatgcactggtaaatagtatagaagcatacacatccgtatatgcttcagaatacacaaacggactagcactagaggcaatacgattaatatttaaatatttgcctgaggcttacaaaaacggaagaaccaatgaaaaagcaagagagaaaatggctcacgcttcaactatggcaggtatggcatccgctaatgcatttctaggtctatgtcattccatggcaataaaattaagttcagaacacaatattcctagtggcattgccaatgcattactaatagaagaagtaataaaatttaacgcagttgataatcctgtaaaacaagccccttgcccacaatataagtatccaaacaccatatttagatatgctcgaattgcagattatataaagcttggaggaaatactgatgaggaaaaggtagatctcttaattaacaaaatacatgaactaaaaaaagctttaaatataccaacttcaataaaggatgcaggtgttttggaggaaaacttctattcctcccttgatagaatatctgaacttgcactagatgatcaatgcacaggcgctaatcctagatttcctcttacaagtgagataaaagaaatgtatataaattgttttaaaaaacaaccttaatactagagattaaagaggagaaatactagatgaaagttacaaatcaaaaagaactaaaacaaaagctaaatgaattgagagaagcgcaaaagaagtttgcaacctatactcaagagcaagttgataaaatttttaaacaatgtgccatagccgcagctaaagaaagaataaacttagctaaattagcagtagaagaaacaggaataggtcttgtagaagataaaattataaaaaatcattttgcagcagaatatatatacaataaatataaaaatgaaaaaacttgtggcataatagaccatgacgattctttaggcataacaaaggttgctgaaccaattggaattgttgcagccatagttcctactactaatccaacttccacagcaattttcaaatcattaatttctttaaaaacaagaaacgcaatattcttttcaccacatccacgtgcaaaaaaatctacaattgctgccgcaaaattaattttagatgcagctgttaaagcaggagcacctaaaaatataataggctggatagatgagccatcaatagaactttctcaagatttgatgagtgaagctgatataatattagcaacaggaggtccttcaatggttaaagcggcctattcatctggaaaacctgcaattggtgttggagcaggaaatacaccagcaataatagatgagagtgcagatatagatatggcagtaagctccataattttatcaaagacttatgacaatggagtaatatgcgcttctgaacaatcaatattagttatgaatccaatatacgaaaaagttaaagaggaatttgtaaaacgaggatcatatatactcaatcaaaatgaaatagctaaaataaaagaaactatgtttaaaaatggagctattaatgctgacatagttggaaaatctgcttatataattgctaaaatggcaggaattgaagttcctcaaactacaaagatacttataggcgaagtacaatctgttgaaaaaagcgagctgttctcacatgaaaaactatcaccagtacttgcaatgtataaagttaaggattttgatgaagctctaaaaaaggcacaaaggctaatagaattaggtggaagtggacacacgtcatctttatatatagattcacaaaacaataaggataaagttaaagaatttggattagcaatgaaaacttcaaggacatttattaacatgccttcttcacagggagcaagcggagatttatacaattttgcgatagcaccatcatttactcttggatgcggcacttggggaggaaactctgtatcgcaaaatgtagagcctaaacatttattaaatattaaaagtgttgctgaaagaagggaaaatatgctttggtttaaagtgccacaaaaaatatattttaaatatggatgtcttagatttgcattaaaagaattaaaagatatgaataagaaaagagcctttatagtaacagataaagatctttttaaacttggatatgttaataaaataacaaaggtactagatgagatagatattaaatacagtatatttacagatattaaatctgatccaactattgattcagtaaaaaaaggtgctaaagaaatgcttaactttgaacctgatactataatctctattggtggtggatcgccaatggatgcagcaaaggttatgcacttgttatatgaatatccagaagcagaaattgaaaatctagctataaactttatggatataagaaagagaatatgcaatttccctaaattaggtacaaaggcgatttcagtagctattcctacaactgctggtaccggttcagaggcaacaccttttgcagttataactaatgatgaaacaggaatgaaataccctttaacttcttatgaattgaccccaaacatggcaataatagatactgaattaatgttaaatatgcctagaaaattaacagcagcaactggaatagatgcattagttcatgctatagaagcatatgtttcggttatggctacggattatactgatgaattagccttaagagcaataaaaatgatatttaaatatttgcctagagcctataaaaatgggactaacgacattgaagcaagagaaaaaatggcacatgcctctaatattgcggggatggcatttgcaaatgctttcttaggtgtatgccattcaatggctcataaacttggggcaatgcatcacgttccacatggaattgcttgtgctgtattaatagaagaagttattaaatataacgctacagactgtccaacaaagcaaacagcattccctcaatataaatctcctaatgctaagagaaaatatgctgaaattgcagagtatttgaatttaaagggtactagcgataccgaaaaggtaacagccttaatagaagctatttcaaagttaaagatagatttgagtattccacaaaatataagtgccgctggaataaataaaaaagatttttataatacgctagataaaatgtcagagcttgcttttgatgaccaatgtacaacagctaatcctaggtatccacttataagtgaacttaaggatatctatataaaatcattttaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z