BBa_I711024
1
BBa_I711024
Crotonyl-CoA -> butyryl-CoA ( butyryl coa dehydrogenase)
2007-07-15T11:00:00Z
2015-08-31T04:07:45Z
Clostridium acetobutylicum ATCC 824
This enzyme is responsible for creating butyryl-CoA from crotonyl-CoA.
true
false
_158_
0
1498
9
Discontinued
false
None.
false
Ranjan Khan
BBa_I711022
1
BBa_I711022
Acetoacetyl-CoA -> beta-hydroxybutyryl-CoA (beta-hydroxybutyryl-CoA dehydrogenase)
2007-07-15T11:00:00Z
2015-08-31T04:07:45Z
Clostridium acetobutylicum ATCC 824
This enzyme is responsible for making beta-hydroxybutyryl-CoA from Acetoacetyl-CoA.
true
false
_158_
0
1498
9
Discontinued
false
None.
false
Ranjan Khan
BBa_I711023
1
BBa_I711023
beta-hydroxybutyryl CoA -> Crotonyl ??? CoA (Crotonase)
2007-07-15T11:00:00Z
2015-08-31T04:07:45Z
Clostridium acetobutylicum ATCC 824
This enzyme is responsible for creating crotonyl-CoA from beta-hydroxybutyryl-CoA.
true
false
_158_
0
1498
9
Discontinued
false
None.
false
Ranjan Khan
BBa_I711025
1
BBa_I711025
Acetoacetyl-CoA -> Butyryl-CoA (through crotonyl-CoA)
2007-07-15T11:00:00Z
2015-08-31T04:07:45Z
Clostridium acetobutylicum ATCC 824
This part creates butyryl-CoA through a series of three enzyme reactions.
true
false
_158_
0
1498
9
Discontinued
false
None.
false
Ranjan Khan
component1938405
1
BBa_J23119
component1938417
1
BBa_I711024
component1938409
1
BBa_I711022
component1938411
1
BBa_B0030
component1938413
1
BBa_I711023
component1938407
1
BBa_B0030
component1938422
1
BBa_B0011
component1938418
1
BBa_B0012
component1938415
1
BBa_B0030
annotation1938418
1
BBa_B0012
range1938418
1
2899
2939
annotation1938415
1
BBa_B0030
range1938415
1
1728
1742
annotation1938405
1
BBa_J23119
range1938405
1
1
35
annotation1938411
1
BBa_B0030
range1938411
1
922
936
annotation1938422
1
BBa_B0011
range1938422
1
2948
2993
annotation1938413
1
BBa_I711023
range1938413
1
943
1719
annotation1938407
1
BBa_B0030
range1938407
1
44
58
annotation1938417
1
BBa_I711024
range1938417
1
1751
2890
annotation1938409
1
BBa_I711022
range1938409
1
65
913
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_I711025_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgaaaaagatttttgtacttggagcaggaacaatgggtgctggtatcgttcaagcattcgctcaaaaaggttgtgaagtaattgtaagagacataaaggaagaatttgttgacagaggaatagctggaatcactaaaggattagaaaagcaagttgctaaaggaaaaatgtctgaagaagataaagaagctatactttcaagaatttcaggaacaactgatatgaaattagctgctgactgtgatttagtagttgaagctgcaatcgaaaacatgaaaattaagaaggaaatcttcgctgaattagatggaatttgtaagccagaagcgattttagcttcaaacacttcatctttatcaattactgaagttgcttcagctacaaagagacctgataaagttatcggaatgcatttctttaatccagctccagtaatgaagcttgttgaaattattaaaggaatagctacttctcaagaaacttttgatgctgttaaggaattatcagttgctattggaaaagaaccagtagaagttgcagaagctccaggattcgttgtaaacagaatattaatcccaatgattaacgaagcttcatttatcctacaagaaggaatagcttcagttgaagatattgatacagctatgaaatatggtgctaaccatccaatgggacctttagctttaggagatcttattggattagacgtttgcttagctatcatggatgttttattcactgaaacaggtgataacaagtacagagctagcagcatattaagaaaatatgttagagctggatggcttggaagaaaatcaggaaaaggattctatgattactctaaataatactagagattaaagaggagaaatactagatggaaaatattatttttaatgaatcaaatggaattgctgaggtaattataaatagacctaaagcattaaatgccttaaacaatcaaactataactgaacttggtgaagttataaatgaaatttcaaaaagaaaagatataaaaactgtaattataacaggagcaggggaaaaggcttttgtagctggtgcagatattgttgaaatgaaagatttaaattcaatggaagcaagagatttttcaagattagctcagaaggtattttcagatatagaaaatatgccacaaattgtaatagctgctgttaatggatatgcattaggcggtggatgtgagctttcaatggcatgtgatataagattagcttctaagaaggctaaatttggacaaccagaagttaatttaggaattcttccaggttttgcaggaactcaaagacttccaagattagttgggaaaggaatagctaaagagcttatattctcaacagatatgattgatgctgaagaagcacatagaataggacttgctaataaggtttatgagccagaagagttaatggacaaggcaagagaattagctaataagataatgagtaagagtccagtaggagttagattagctaaggcagcaataaacaacggcttaaacatggatactgaatcagcatacaactatgaggcagatttatttgctttatgtttctcaacagaagatcaattagaaggtatgaatgcctttgttgataagagaaaagctgattttaaagataaataatactagagattaaagaggagaaatactagagttatcttaatatgcttcctgcgataaccattttttgaacttctgaagttccttcgtaaatctcagttatcttagcatctctcatcattctttctactgggtattccttagtatatccatatccaccgaataattgaactgccttagttgtaacttccatagctacatctgcagcgaataatttagctcttgcagcatctatagaatatggtttaccctcttcttttaaacaagctgctttatatactaaaaatcttgcagcttctattttagtatccatttctgctaccatccattgtaatccttggaatgcagataatgatctaccaaattgttttctttctttcatgtaagcaacagcttcttctaaagctccttctgctatacctaatgcttgagcagcgatacctattctaccaccgtctaaagttttcattgctattccaaatccttttccttctcttccgattaagttttcttttggaacaacacagttttccatgattaactcagttgttgaagaagctcttatacccattttttcttctaacttaccaattgagaatcctgggaaattcttttcaactatgaaagctgaaattcctcttgtaccttgactcttatcagtcatagcaaagattatgaaagtatctgcaactccaccattagttatgaagattttgcttccgtttaagatataattatctccttctaatacagctgtagtttgttgtcctgccgcatctgtacctgctcctggttcagttaatccaaaagcacctaatttttcaccttttgcaagtggagttaaatatttctctttttgagctggagtaccaaattgatctattagtgaagcacataatgaagtatgagctgaaactataactcctgtagtagcacaaacttttgataattcttcaacagttaagatgtaagataaagtatctccaccagctccgccatattctttgctaaaaggaattcccatcattcctagtttagccattttcttaacattttccataggaaatctttctgttacatctatttctgcagctataggcttaacttcattttgagcaaattctctaaccatttgcttaactaattcttgttctcttgttaattgaaagttcattactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_I711023_sequence
1
atggagctgaacaatgtaatcctggaaaaagaaggtaaagttgccgttgtaaccatcaatcgcccgaaagcgctgaacgctctgaactccgacaccctgaaggagatggattacgttatcggtgaaattgaaaacgactctgaagtgctggctgttatcctgactggtgccggcgaaaaatctttcgttgccggcgctgacatcagcgaaatgaaggaaatgaataccatcgagggccgtaaattcggtatcctgggcaacaaggtgtttcgtcgcctggaactgctggaaaagcctgttatcgcggctgtcaatggtttcgcactgggtggcggttgtgaaatcgcaatgtcttgcgacattcgtatcgcttcctctaacgctcgttttggtcaacctgaggtcggcctgggtattactccgggttttggcggtacccagcgtctgtcccgcctggttggtatgggtatggctaaacagctgattttcaccgctcagaacatcaaagctgatgaagcgctgcgcatcggcctggttaacaaagtggtggaaccatctgaactgatgaacaccgcaaaggaaatcgcaaacaaaatcgtatccaatgccccagttgctgtaaagctgtctaaacaggctatcaaccgtggcatgcagtgcgacatcgataccgcactggcgtttgaaagcgaagctttcggtgaatgtttcagcaccgaggatcagaaagacgcgatgaccgctttcatcgaaaagcgtaagattgaaggtttcaaaaaccgt
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_I711022_sequence
1
atgaaaaaggtttgtgttattggcgcaggtaccatgggttctggcatcgcccaggctttcgcagcgaaaggtttcgaagttgtgctgcgcgacattaaagacgagttcgttgatcgtggtctggacttcatcaacaaaaatctgtccaagctggtgaaaaagggtaaaatcgaagaagcaactaaagttgagatcctgacccgtatctctggtactgtggatctgaacatggcggcagattgcgacctggtcattgaggctgcggttgaacgtatggatatcaagaaacaaattttcgctgacctggataacatctgcaaaccggaaaccattctggcttccaacaccagctccctgtccatcactgaagtggcttctgcaacgaaacgtccggataaagtcatcggtatgcacttcttcaatccggctccggttatgaaactggtggaagtgatccgtggtatcgcaacgagccaggagactttcgacgccgttaaagaaacttctatcgcaatcggtaaagatccggtagaggttgctgaagcgccaggtttcgttgtaaaccgtatcctgattccgatgatcaatgaagcggtaggtattctggctgaaggcattgcatccgtggaagatatcgacaaagcaatgaaactgggtgcaaaccacccgatgggtccgctggaactgggtgactttatcggcctggatatttgtctggcgattatggacgttctgtattctgaaactggcgactccaaatatcgcccgcacaccctgctgaagaaatatgttcgtgcgggttggctgggccgtaaatctggcaaaggtttttacgactactctaag
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_I711024_sequence
1
atggactttaacctgactcgtgaacaagaactggtacgccaaatggttcgtgagttcgcggaaaatgaagtgaaaccgatcgcagctgaaattgatgaaacggaacgcttcccgatggaaaacgtaaagaaaatgggtcagtacggcatgatgggtatcccgttctccaaagaatatggcggcgcaggcggtgacgtcctgagctacatcattgctgtcgaggagctgtccaaggtctgcggcactaccggcgtgatcctgagcgcgcatacctccctgtgcgcctccctgatcaacgaacacggtaccgaggaacagaaacaaaaatatctggttccgctggcgaagggtgaaaaaatcggtgcttatggcctgaccgaaccgaatgcgggtaccgactctggtgcgcagcagactgtcgcggtcctggagggtgatcattatgttattaacggttctaagatcttcatcacgaacggcggtgtggcagacaccttcgtgatttttgcaatgacggaccgcacgaaaggcaccaaaggtatctctgctttcatcatcgagaaaggctttaagggcttttctattggtaaagtagaacagaaactgggcattcgtgcatccagcactacggagctggtctttgaagatatgatcgttccggtagaaaacatgattggcaaggaaggtaaaggcttcccgattgccatgaaaactctggacggtggccgcatcggcattgcggctcaggccctgggtatcgcggaaggtgcattcaacgaagcacgcgcttatatgaaagagcgtaaacagttcggccgtagcctggacaaattccagggtctggcatggatgatggccgacatggacgttgctatcgagtccgctcgttatctggtttataaagcggcctatctgaaacaggcgggtctgccttatactgtggatgccgcccgtgcgaaactgcacgcggctaacgttgctatggatgttaccactaaggctgtacagctgttcggcggctacggttacactaaagactaccctgtagaacgtatgatgcgtgacgcgaaaatcactgaaatctatgaaggtacgtctgaggttcagaagctggttatcagcggcaaaattttccgt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z