LOCUS BBa_I711031 3958 bp DNA linear 29-Nov-2024 DEFINITION Cellobiohydrolase & cellbiase (cellulose -> beta-D-glucose ACCESSION BBa_I711031 VERSION BBa_I711031.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 CDS 65..2440 /label=BBa_I711029 RBS 2449..2463 /label=RBS-1\Strong RBS 2456..2460 /label=RBS misc_feature 2449..2463 /label=BBa_B0030 RBS 2449..2463 /label=BBa_B0030 CDS 2470..3855 /label=BBa_I711030 stem_loop 3871..3890 /label=T7 TE misc_feature 3897..3897 /label=stop polyA_site 3891..3904 /label=polya misc_feature 3864..3904 /label=BBa_B0012 terminator 3864..3904 /label=BBa_B0012 stem_loop 3925..3947 /label=stem_loop misc_feature 3913..3958 /label=BBa_B0011 terminator 3913..3958 /label=BBa_B0011 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagattaaag aggagaaata 61 ctagatgttg gcttctaata aaaatagtaa gctggcaaac tctgagcaac accgccctta 121 taaaacccgc acagcgcgct ggttaaccgg gtctggggtt attgcttcaa gtttgctttt 181 ttctgcgcag agttttgcgg cgcaatgtga atacatcatt agcaatgaat ggaacagcgg 241 ctttactggc gcagttcgca ttactaataa tggcactact cccatcaatg gctgggatgt 301 tagctggcag tatgccggcg atgcagtcac cagcagctgg aacgcgaatg tttctggctc 361 gaaccccgtt tctgctacac cattaagctg gaatgccaac attcaacccg gtcaaagcgt 421 tgagtttggt tttcagggca gcaaagccgg ctccaatgca gaaattccaa ccgttaccgg 481 cgcggtatgt gatagcggct ctagctcttc cagctccagc tcatcatcta gttcatcaag 541 ctcttctagt agctcaagca gcactagcag ctcctcgtcc agctcttcaa gcacctcttc 601 gtctagctca tcatctggct ccagtggcac aggtggtatt gcgtgtactg taggcaatgc 661 gaatatttgg ggctcgggct accagctgga catgcaagtt gttaacaacg gcaccgctgc 721 agtaagcagt tgggacgtaa ccatggcatt cggcgaggca ccacagcgca ccggtggctg 781 gaacgcaaac tttgtagagt caggcaatac cattgttgcg agcaacatta gctggaacgg 841 caacctcgca ccggggcaat cagcttcgtt tggtattcaa gggaaccacg acggctcttt 901 tggcggcgta acctgtaacg gcgcttcaag ctctggctcg tctagttctg gctctagcac 961 cagctcatca agtagctcat ccagttcgtc tggctctagc acttctagct ctagctcaag 1021 ctcctctact ggttctacct ctagctctag tagctcttca acttctagca cttcttcaag 1081 ttctagctct agcaccagct ccacgagttc tagctccagt tctagctcga gtacatcgtc 1141 tagttccagc tcatcttcct caagctctag ctcttctact tcaggcagtg gcgcaggttt 1201 tgacaacccg ttcattggcg gcaagtggta tgtagaccca gtatggtcag caaaagctgc 1261 agcagagcca aacggttcac ttattgccaa ctacaacacg gcagtttgga tggatcgcat 1321 tggtgcgatt gaaggcccag aagatggcga tggtatgggc ttagaagaac acttagatga 1381 agctttagca caaggtgcag acatctttat gttcgtggta tacgacctac caaaccgcga 1441 ctgtgcagct ttggcctcaa gtggcgaact actcattgcc gagaacggtt ttgagcgcta 1501 tcaaaatgag tacattggcc caatcgtaga tatactcagc aagcccgcgt attctagctt 1561 gcgtattatc gcgattattg aagtggattc tctacccaac ctcgttacca acctcaacat 1621 tcaaaaatgt gttgaagcga atggcccggg tgggtacgta gacggtatcc aacatgcact 1681 taacgagcta aacacgcttg ataatgtgta cccatacgtc gatattgctc actcaggctg 1741 gctaggctgg agcgacaact tcgccggcgc caccaagctt attggtgatg caattaaagg 1801 cacaaacaaa ggtgtaaaca gtattgcagg ctttgtaagt aactcttcta actacacacc 1861 tgtgactgaa ccatacctac ctaaccctac cttgcaaatt ggtagcaacc aagttcgatc 1921 tgcggatttc tacgagtgga ccatgtactt cgaagaactt agctttgtac aagattggcg 1981 ccaagccatg attcagcaag gcttcccaga atcaattggt atgcttattg ataccgcacg 2041 taatggctgg ggtggacctg accgtccaac tggtgagtct acatctaccg acctcaacac 2101 ctatgtgaat gaatcgcgta tagaccgccg tcagcatcgc ggaaactggt gtaaccagcc 2161 cggtggtgtt ggcttccgtc cgcaagcggc accagaacca ggtgtagacg cttacgtttg 2221 ggttaagcca caaggtgagt cggatggtat tagtgatcct aacttcccta tcgaccctaa 2281 cgacccagct aaacagcacg acccaatgtg tgatccaaac gcacctaacc gcgataacaa 2341 tgcggttggc acaggcgcgc tagataacgc tccacatgct ggtcgctggt tcccagaagc 2401 attccaaata cttatagaaa acgcctaccc accgctatag tactagagat taaagaggag 2461 aaatactaga tgaaaacctt taacccagat ttcgtatggg gagcagccag ttccgcctat 2521 caggtagaag gcgccaccac caccgatggc agaggcccca gtatttggga tgcgttcagt 2581 tccattcccg gtaaaaccta ccacaaccaa aacgccgaca tagcctgcga ccactacaac 2641 cgctggcaag aagacgtggc cataatgaaa gagatggggc taaaggctta ccgcttttct 2701 atttcttggt cgcgcatatt ccctactggg cgcggcgaag ttaacgaaaa aggcgtagcc 2761 ttttacaaca accttatcga cgaattaata aaaaacgaca ttaccccttg ggtaacccta 2821 tttcactggg actttcctct ggcactgcaa atggaaatgg acggcctact taaccccgcc 2881 atcgccgacg aattcgccaa ctacgccaag ctgtgtttcg cgcgctttgg cgaccgcgtt 2941 acccactgga ttaccctaaa cgaaccttgg tgcagtgcca tgcttggcca cggcatgggc 3001 agcaaagccc ctggccgcgt atctaaggat gaaccctata tagccgccca caacttgctg 3061 cgtgcacacg gcaaaatggt agatatttac cggcgcgaat ttcagcccac acaaaaaggc 3121 atgataggca tagccaacaa ttgcgactgg cgcgaaccca aaaccgattc tgaattagat 3181 aaaaaagcag ccgagcgcgc cctagaattt tttgtaagct ggtttgccga ccccatttat 3241 ttgggcgact acccagccag catgcgcgag cgcttgggtg agcgtttacc cacctttagc 3301 gacgaagaca ttgcgctaat aaaaaactct agcgactttt ttggtttgaa tcactacacc 3361 accatgcttg ccgaacaaac ccacgaaggt gacgttgttg aagatactat tcgcggcaac 3421 ggcggcatat cggaagacca aatggtcacc ctctccaaag acccaagctg ggaacaaacc 3481 gacatggagt ggagcattgt gccctggggc tgtaaaaaat tattaatctg gttaagcgag 3541 cgctacaact accccgacat ttacattacc gaaaacggct gcgccctacc cgacgaagac 3601 gacgtaaaca tagccattaa cgatacacgc cgcgtagatt tttaccgcgg ttatatcgat 3661 gcgtgtcacc aagcaataga ggccggcgta aaactaaaag gctattttgc atggacactt 3721 atggataact acgaatggga agaaggctac accaaacgct ttggcttaaa ccatgtagat 3781 ttcaccacag gcaaacgcac acctaaacag tctgcaattt ggtatagcac gttaattaaa 3841 gatggtgggt tctagtacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 3901 tatatactag agagagaata taaaaagcca gattattaat ccggcttttt tattattt //