BBa_I711030 1 BBa_I711030 cellobiose -> beta-D-glucose (cellobiase) (bgl3C) 2007-07-19T11:00:00Z 2015-08-31T04:07:45Z Saccharophagus degradans 2-40 This sequence codes for an enzyme that hydrolyzes the terminal, non-reducing beta-D-glucose residues which releases beta-D-glucose. false false _158_ 0 1498 9 Not in stock false None. false Ranjan Khan BBa_I711029 1 BBa_I711029 cellulose -> cellobiose (cellobiohydrolase) (cel6A) 2007-07-19T11:00:00Z 2015-08-31T04:07:45Z Saccharophagus degradans 2-40 This sequence codes for a cellobiohydrolase that hydrolyzies 1,4,-bet-D-glucosidic linkages in cellulose and celltetraose, releasing cellobiose from the non-reducing ends. false false _158_ 0 1498 9 Not in stock false None. false Ranjan Khan BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation1683 1 stem_loop range1683 1 13 35 annotation7019 1 BBa_B0011 range7019 1 1 46 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_I711031 1 BBa_I711031 Cellobiohydrolase & cellbiase (cellulose -> beta-D-glucose 2007-07-19T11:00:00Z 2015-08-31T04:07:45Z Saccharophagus degradans 2-40 This part is composed of two genes that code for enzymes that break down cellulose into glucose. true false _158_ 0 1498 9 Discontinued false None. false Ranjan Khan component1938997 1 BBa_B0030 component1939004 1 BBa_B0011 component1938993 1 BBa_B0030 component1938999 1 BBa_I711030 component1938991 1 BBa_J23119 component1938995 1 BBa_I711029 component1939000 1 BBa_B0012 annotation1938995 1 BBa_I711029 range1938995 1 65 2440 annotation1938997 1 BBa_B0030 range1938997 1 2449 2463 annotation1939004 1 BBa_B0011 range1939004 1 3913 3958 annotation1938993 1 BBa_B0030 range1938993 1 44 58 annotation1938991 1 BBa_J23119 range1938991 1 1 35 annotation1939000 1 BBa_B0012 range1939000 1 3864 3904 annotation1938999 1 BBa_I711030 range1938999 1 2470 3855 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_B0030_sequence 1 attaaagaggagaaa BBa_I711029_sequence 1 atgctggcttctaataagaactccaaactggcaaactctgaacagcaccgtccgtacaaaactcgcaccgcccgctggctgactggctccggcgtcattgcatcctctctgctgttctccgcacagagcttcgccgcacagtgcgagtatatcatttctaatgaatggaactccggcttcaccggtgccgtacgtatcactaataacggcactacgccgatcaacggctgggacgtttcttggcagtacgcaggtgacgcagtcacgtcctcctggaatgctaacgtgtctggttccaacccggtatccgcgactccgctgtcttggaacgcgaacatccagccgggtcagagcgtagaatttggctttcagggttccaaagctggctctaacgcggaaatcccgaccgttactggcgcagtttgcgactctggctcttctagcagcagctctagctcttctagctcctcctcctctagctcttcctccagctcctccacgtctagctccagctcttcttctagcagcacctccagctcttcctctagctctggctcttccggtacgggtggtatcgcttgtactgtgggcaacgcaaacatttggggttccggttatcagctggacatgcaggtggttaacaacggcactgcggccgtaagctcctgggatgtgactatggctttcggtgaggcgccgcaacgtaccggcggctggaacgcgaacttcgttgaatccggcaatactatcgttgcctctaatattagctggaacggtaacctggctccgggtcagtccgcgtcctttggtatccagggcaaccatgacggcagctttggtggtgtgacctgcaatggtgcgagctcctccggctcctcctctagcggttcctccacctccagcagctcctcttcctcttcttcttctggcagctccacctcctcttctagctctagctctagcacgggttctacgtcctctagctcctcttcctctaccagctctacgagctcttcctccagcagctctacctcttccactagctcctcctcttcctcttccagcagcacctctagcagctcctcctcttcttccagcagcagcagctcttctacctccggcagcggtgcaggtttcgataacccgttcattggtggcaaatggtatgttgatccggtatggtccgcgaaagccgctgcggagcctaacggttctctgatcgcaaactataacacggctgtatggatggatcgtattggtgcgatcgagggcccggaagacggtgacggcatgggcctggaagaacacctggatgaggctctggcgcagggcgcagacatcttcatgttcgttgtatatgacctgccaaaccgtgattgtgccgcgctggcaagcagcggtgaactgctgatcgcagaaaacggcttcgaacgttaccagaacgagtacattggccctatcgtggatatcctgagcaaaccggcttacagcagcctgcgtattatcgcaatcatcgaagttgattccctgcctaacctggtaaccaacctgaacatccaaaaatgtgtagaagctaacggtccgggtggttatgtcgatggcatccaacacgcgctgaacgaactgaacactctggacaacgtgtacccatacgtggatatcgcccacagcggttggctgggctggtccgacaacttcgcaggcgctaccaaactgatcggcgatgcgatcaaaggtacgaacaagggtgtcaactccatcgctggttttgtttccaactccagcaactatacgcctgttacggaaccttacctgccgaacccgaccctgcaaattggctctaaccaggtgcgttctgccgatttttatgaatggaccatgtatttcgaggaactgtctttcgttcaggattggcgccaagccatgattcagcagggcttcccggaaagcattggcatgctgattgacaccgcgcgcaatggttggggcggtccagaccgtccgacgggtgaaagcacctctaccgatctgaacacttatgtaaacgaatcccgtattgaccgtcgtcagcatcgtggcaactggtgcaaccagccgggtggcgtcggtttccgtccgcaggccgctccggaacctggtgtagacgcgtacgtatgggtgaaaccacagggtgaaagcgacggcatctctgatccgaacttcccgattgaccctaatgacccggctaaacagcatgatccaatgtgtgaccctaacgcaccgaaccgtgacaacaacgcagtaggtaccggcgcgctggacaacgcaccgcacgcaggccgttggttcccggaagcattccaaatcctgatcgagaacgcttatccgccgctg BBa_I711031_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgttggcttctaataaaaatagtaagctggcaaactctgagcaacaccgcccttataaaacccgcacagcgcgctggttaaccgggtctggggttattgcttcaagtttgcttttttctgcgcagagttttgcggcgcaatgtgaatacatcattagcaatgaatggaacagcggctttactggcgcagttcgcattactaataatggcactactcccatcaatggctgggatgttagctggcagtatgccggcgatgcagtcaccagcagctggaacgcgaatgtttctggctcgaaccccgtttctgctacaccattaagctggaatgccaacattcaacccggtcaaagcgttgagtttggttttcagggcagcaaagccggctccaatgcagaaattccaaccgttaccggcgcggtatgtgatagcggctctagctcttccagctccagctcatcatctagttcatcaagctcttctagtagctcaagcagcactagcagctcctcgtccagctcttcaagcacctcttcgtctagctcatcatctggctccagtggcacaggtggtattgcgtgtactgtaggcaatgcgaatatttggggctcgggctaccagctggacatgcaagttgttaacaacggcaccgctgcagtaagcagttgggacgtaaccatggcattcggcgaggcaccacagcgcaccggtggctggaacgcaaactttgtagagtcaggcaataccattgttgcgagcaacattagctggaacggcaacctcgcaccggggcaatcagcttcgtttggtattcaagggaaccacgacggctcttttggcggcgtaacctgtaacggcgcttcaagctctggctcgtctagttctggctctagcaccagctcatcaagtagctcatccagttcgtctggctctagcacttctagctctagctcaagctcctctactggttctacctctagctctagtagctcttcaacttctagcacttcttcaagttctagctctagcaccagctccacgagttctagctccagttctagctcgagtacatcgtctagttccagctcatcttcctcaagctctagctcttctacttcaggcagtggcgcaggttttgacaacccgttcattggcggcaagtggtatgtagacccagtatggtcagcaaaagctgcagcagagccaaacggttcacttattgccaactacaacacggcagtttggatggatcgcattggtgcgattgaaggcccagaagatggcgatggtatgggcttagaagaacacttagatgaagctttagcacaaggtgcagacatctttatgttcgtggtatacgacctaccaaaccgcgactgtgcagctttggcctcaagtggcgaactactcattgccgagaacggttttgagcgctatcaaaatgagtacattggcccaatcgtagatatactcagcaagcccgcgtattctagcttgcgtattatcgcgattattgaagtggattctctacccaacctcgttaccaacctcaacattcaaaaatgtgttgaagcgaatggcccgggtgggtacgtagacggtatccaacatgcacttaacgagctaaacacgcttgataatgtgtacccatacgtcgatattgctcactcaggctggctaggctggagcgacaacttcgccggcgccaccaagcttattggtgatgcaattaaaggcacaaacaaaggtgtaaacagtattgcaggctttgtaagtaactcttctaactacacacctgtgactgaaccatacctacctaaccctaccttgcaaattggtagcaaccaagttcgatctgcggatttctacgagtggaccatgtacttcgaagaacttagctttgtacaagattggcgccaagccatgattcagcaaggcttcccagaatcaattggtatgcttattgataccgcacgtaatggctggggtggacctgaccgtccaactggtgagtctacatctaccgacctcaacacctatgtgaatgaatcgcgtatagaccgccgtcagcatcgcggaaactggtgtaaccagcccggtggtgttggcttccgtccgcaagcggcaccagaaccaggtgtagacgcttacgtttgggttaagccacaaggtgagtcggatggtattagtgatcctaacttccctatcgaccctaacgacccagctaaacagcacgacccaatgtgtgatccaaacgcacctaaccgcgataacaatgcggttggcacaggcgcgctagataacgctccacatgctggtcgctggttcccagaagcattccaaatacttatagaaaacgcctacccaccgctatagtactagagattaaagaggagaaatactagatgaaaacctttaacccagatttcgtatggggagcagccagttccgcctatcaggtagaaggcgccaccaccaccgatggcagaggccccagtatttgggatgcgttcagttccattcccggtaaaacctaccacaaccaaaacgccgacatagcctgcgaccactacaaccgctggcaagaagacgtggccataatgaaagagatggggctaaaggcttaccgcttttctatttcttggtcgcgcatattccctactgggcgcggcgaagttaacgaaaaaggcgtagccttttacaacaaccttatcgacgaattaataaaaaacgacattaccccttgggtaaccctatttcactgggactttcctctggcactgcaaatggaaatggacggcctacttaaccccgccatcgccgacgaattcgccaactacgccaagctgtgtttcgcgcgctttggcgaccgcgttacccactggattaccctaaacgaaccttggtgcagtgccatgcttggccacggcatgggcagcaaagcccctggccgcgtatctaaggatgaaccctatatagccgcccacaacttgctgcgtgcacacggcaaaatggtagatatttaccggcgcgaatttcagcccacacaaaaaggcatgataggcatagccaacaattgcgactggcgcgaacccaaaaccgattctgaattagataaaaaagcagccgagcgcgccctagaattttttgtaagctggtttgccgaccccatttatttgggcgactacccagccagcatgcgcgagcgcttgggtgagcgtttacccacctttagcgacgaagacattgcgctaataaaaaactctagcgacttttttggtttgaatcactacaccaccatgcttgccgaacaaacccacgaaggtgacgttgttgaagatactattcgcggcaacggcggcatatcggaagaccaaatggtcaccctctccaaagacccaagctgggaacaaaccgacatggagtggagcattgtgccctggggctgtaaaaaattattaatctggttaagcgagcgctacaactaccccgacatttacattaccgaaaacggctgcgccctacccgacgaagacgacgtaaacatagccattaacgatacacgccgcgtagatttttaccgcggttatatcgatgcgtgtcaccaagcaatagaggccggcgtaaaactaaaaggctattttgcatggacacttatggataactacgaatgggaagaaggctacaccaaacgctttggcttaaaccatgtagatttcaccacaggcaaacgcacacctaaacagtctgcaatttggtatagcacgttaattaaagatggtgggttctagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_I711030_sequence 1 atgctgctgtctctgaaaaatactcagctgaaacgctctatgaacatgaacctgaaacatctgttcctggttgctctggctctgaacatcgcggcttgtaacgttaaagaaccggcagcgaccaacgacaaccacatctcctatcaggcagcccgtgaagcgcgtctggccaaagttgaagctgaagtggaacgcctgctgccgctgctgaccctggaagagaaagcaagcctggttcatgctaacagcaaattctctattgcgtctattgaacgtctgggtatccacgaaatgtggatgtctgacggcccgcatggcgtacgttatcagatcgaacgtcatggctgggccccagctggctggaccgacgataactctacctacctgccaccactgaccactgtcgcggcatcttggaacccagaaatcgcggctctgcacggtgacgtactgggtgctgaagcacgccaccgtcgcaaagacgttatcctgggtccgggtgttaacctggcccgtctgccactgtacggccgtaacttcgaatacatgggtgaagacccgttcctggcaagccgtctggcggtggcggaaattaaagccattcaagagaacgacgtggccgcctgcattaaacacttcgcactgaacaaccaggaactgaaccgtacgggtgtgaacgctaaaccggacgaacgtacgctgcgtgaagtttatctgccggcgttcgaagcagcagtaaaggaagcaggcgtccacacgatcatgggtgcgtacaacgaatttcgtggcaccaatgctaaccagtctaaacacctggtgatggacattctgaaaggtgaatggggctataaaggcgttctgctgactgattggaacgttgacattaacacctatgacgcggccgtaaatggcctggacattgaaatgggtaccaatgtcgacagctacgatgactatatgctggcacagccaatgattgacatgattaaagccggttccatcggcggtggtagctccgaagtaccggccctgtacgaaatcaccccactgcaaggtctgaagcagaaactgggcgacaacgtgaatatcacggtaatgcgtgcacgctatgatggtgttctgatgccgatcgcctccgattacgtaacttcccgccattggaccggtactccagcttggaacatggtgcgttactctgatgccgctcgtacccaggcgatcggcgacagcgcgattgttgattctgcctattcctctccggctggcaccaccaaagagtatgtaactatgactgcgaccatcaagccactgaaatctggtgagcacaccctgaaaacttctgtgatgggcgacttcgaactgaaaattaacggtaaaacgaccgtgaagcattctagcacctccggtgacgtcgttactcagaaaattgcgctgaacggcggtgaaacctactcttttgaaatcctgtattctggtaacaagaacttcacgctgggctgggatgcaccaggcgacctgttcaccgcagaaaaagaatacatcgctgcggcgaagaaagccgacgttgttttctactttggcggcctgacccacggcgacgaccgcgaagcgatcgaccgcccgcatatgaagctgccaaaccaccaggatccggtgatcagcaaagtactggctgcgaacccgaacaccgttgtcttcctgatcgccggctccgcagttgagatgccatgggccgataaagcgaaagcaattgtttggggctggtatggcggcatggaagcaggcaacgcttacgctgatatgctgttcggcgacaccaatccgtccggtaagatgccgatcaccctgccgaaagctctggaagacaccgccccaatcgctctgaacgactacaacccggttgagtccctgtatactgaaggcgttttcatcggttaccgttggttcgaaaaacagaacattgagccgctgttcccgttcggtcacggtctgagctatacccagttcaaatataacaacatcaagctgtctagcgctaacatcaaaggtgatcagaccgttacggtgagcgcgaccattaccaatactggcaaagttgctggtgcggaggtagtgcaactgtatctgcacgacgaacaagcctctgttgaacgtccggctaaagaactgaaaggttttcagaaggtattcctgaaaccgggtgaaagcaaagccgtaaacattaccctgaacaaacgtgccctgtccttctgggacgaaaactccaacgactggctggcagagactggcaaattcaacgtactgctgggtgcttccgtatctgacattcgcctgcaaacctcctttcagtaccaacaa BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z