BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_I711040
1
BBa_I711040
proteorhodopsin generator
2007-10-08T11:00:00Z
2015-08-31T04:07:45Z
blah
blah
false
false
_158_
0
1498
9
It's complicated
false
blah
false
Ranjan Khan
component1945605
1
BBa_I711033
component1945601
1
BBa_J23119
component1945606
1
BBa_B0012
component1945603
1
BBa_B0030
component1945610
1
BBa_B0011
annotation1945606
1
BBa_B0012
range1945606
1
844
884
annotation1945605
1
BBa_I711033
range1945605
1
65
835
annotation1945601
1
BBa_J23119
range1945601
1
1
35
annotation1945603
1
BBa_B0030
range1945603
1
44
58
annotation1945610
1
BBa_B0011
range1945610
1
893
938
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_I711033
1
BBa_I711033
Proteorhodopsin (light-activated proton pump)
2007-10-08T11:00:00Z
2015-08-31T04:07:45Z
Marine metagenomic analysis
This sequence codes for a protein that works with retinal to pump protons out of the cell in the presence of light.
false
false
_158_
0
1498
9
Not in stock
false
Codon optimized for E. coli.
false
Ranjan Khan
BBa_I711040_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgtccatcattaaatctctgaaaagcgtcgcaatcgcatctctggcgatcctgatcccgtctatcgctctggctgcgggtggcaacctggaaccgaacgacccggtaggtattaccttctggctgatttccatcgctatggttgcggcgaccgtctttttcctgatggaatccctgcgtgtagacggcaaatggcgtacctctatgattgttggtggtctggtgaccctggtggcggcggtacattacttctacatgcgcgacgtgtgggttgctaccggcgcatctccgaccgtgtttcgctacgttgactggctgatcactgttccgctgcaaatgatcgaattttatctgatcctggcggcatgcaccgctatcgctgtgggcgttttctggcgtctgatgattggcaccatggtcatgctgattggtggttacctgggcgaggcaggttttatcaacgctaccgtcggtttcgttatcggtatggctggttggggctacatcctgtacgaaatctttgcaggcgaagcgggtaaagtggccgcggagggtgcaccgccgtctgttcagtccgcgttcaacactatgcgcctgatcgttaccattggttgggccatttacccactgggctacttttttggttacatgaccggcggtgttgatgcgaactctctgaacctgatctacaacgttgcggatgtggttaacaaaatcggcttctgtctggcaatttgggcggctgcgacctctcagtccgaagctgcgaaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_I711033_sequence
1
atgtccatcattaaatctctgaaaagcgtcgcaatcgcatctctggcgatcctgatcccgtctatcgctctggctgcgggtggcaacctggaaccgaacgacccggtaggtattaccttctggctgatttccatcgctatggttgcggcgaccgtctttttcctgatggaatccctgcgtgtagacggcaaatggcgtacctctatgattgttggtggtctggtgaccctggtggcggcggtacattacttctacatgcgcgacgtgtgggttgctaccggcgcatctccgaccgtgtttcgctacgttgactggctgatcactgttccgctgcaaatgatcgaattttatctgatcctggcggcatgcaccgctatcgctgtgggcgttttctggcgtctgatgattggcaccatggtcatgctgattggtggttacctgggcgaggcaggttttatcaacgctaccgtcggtttcgttatcggtatggctggttggggctacatcctgtacgaaatctttgcaggcgaagcgggtaaagtggccgcggagggtgcaccgccgtctgttcagtccgcgttcaacactatgcgcctgatcgttaccattggttgggccatttacccactgggctacttttttggttacatgaccggcggtgttgatgcgaactctctgaacctgatctacaacgttgcggatgtggttaacaaaatcggcttctgtctggcaatttgggcggctgcgacctctcagtccgaagctgcgaaa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z