BBa_I712003 1 BBa_I712003 CCR5-NUb 2007-10-18T11:00:00Z 2015-08-31T04:07:45Z CCR5 gene obtained from Origene, NUb gene obtained from Dualsystems. Chemokine (C-C motif) receptor 5, fused to N-terminal half of ubiquitin. false false _130_ 0 1896 9 It's complicated false PstI and EcoRI restriction sites inside CCR5 gene were mutated without changing the aminoacid sequence. CCR5 and NUb fragments were fused with PCR overlap extension method. The part is cloned into BBa_J52017, a vector with eukaryontic terminator. false Andrej Ondracka annotation1950099 1 NUb range1950099 1 1057 1194 annotation1950098 1 CCR5 range1950098 1 1 1056 BBa_I712003_sequence 1 atggattatcaagtgtcaagtccaatctatgacatcaattattatacatcggagccctgccaaaaaatcaatgtgaagcaaatcgcagcccgcctcctgcctccgctctactcactggtgttcatctttggttttgtgggcaacatgctggtcatcctcatcctgataaactgcaaaaggctgaagagcatgactgacatctacctgctcaacctggccatctctgacctgtttttccttcttactgtccccttctgggctcactatgctgccgcccagtgggactttggaaatacaatgtgtcaactcttgacagggctctattttataggcttcttctctggaatcttcttcatcatcctcctgacaatcgataggtacctggctgtcgtccatgctgtgtttgctttaaaagccaggacggtcacctttggggtggtgacaagtgtgatcacttgggtggtggctgtgtttgcgtctctcccaggaatcatctttaccagatctcaaaaagaaggtcttcattacacttgcagctctcattttccatacagtcagtatcaattctggaagaatttccagacattaaagatagtcatcttggggctggtcctgccgctgcttgtcatggtcatctgctactcgggaatcctaaaaactctgcttcggtgtcgaaatgagaagaagaggcacagggctgtgaggcttatcttcaccatcatgattgtttattttctcttctgggctccctacaacattgtccttctcctgaacaccttccaggagttctttggcctgaataattgcagtagctctaacaggttggaccaagctatgcaggtgacagagactcttgggatgacgcactgctgcatcaaccccatcatctatgcctttgtcggggagaagttcagaaactacctcttagtcttcttccaaaagcacattgccaaacgcttctgcaaatgctgttctattttccagcaagaggctcccgagcgagcaagctcagtttacacccgatccactggggagcaggaaatatctgtgggcttgatgcagattttcgtcaagactttgaccggtaaaaccggaacattggaagttgaatcttccgataccatcgacaacgttaagtcgaaaattcaagacaaggaaggaatccctggtggatgtcgacctcgagtcatgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z