BBa_I712003 1 BBa_I712003 CCR5-NUb 2007-10-18T11:00:00Z 2015-08-31T04:07:45Z CCR5 gene obtained from Origene, NUb gene obtained from Dualsystems. Chemokine (C-C motif) receptor 5, fused to N-terminal half of ubiquitin. false false _130_ 0 1896 9 It's complicated false PstI and EcoRI restriction sites inside CCR5 gene were mutated without changing the aminoacid sequence. CCR5 and NUb fragments were fused with PCR overlap extension method. The part is cloned into BBa_J52017, a vector with eukaryontic terminator. false Andrej Ondracka annotation1950099 1 NUb range1950099 1 1057 1194 annotation1950098 1 CCR5 range1950098 1 1 1056 BBa_I712007 1 c-Myc c-Myc affinity tag 2007-10-19T11:00:00Z 2015-08-31T04:07:45Z Constructed by primer annealing. c-myc tag is used for detection of proteins by anti-c-myc antibodies. false false _130_ 0 1846 9 It's complicated false Gene was designed to be constructed by primer annealing. Includes start codone. false Anja Korenčič BBa_I712006 1 BBa_I712006 CMV + Myc + CCR5-NUb 2007-10-21T11:00:00Z 2015-08-31T04:07:45Z For details, please check CMV, c-Myc-tag and CCR5-NUb basic parts. CCR5-NUb under control of CMV promoter. c-Myc-tag is added on extracellular domain for easier detection. false false _130_ 0 1896 9 In stock true The part is cloned into BBa_J52017 vector (pSBAK3 vector with eukaryotic terminator). true Andrej Ondracka component1952285 1 BBa_I712003 component1952281 1 BBa_I712004 component1952282 1 BBa_I712007 annotation1952281 1 BBa_I712004 range1952281 1 1 654 annotation1952282 1 BBa_I712007 range1952282 1 661 690 annotation1952285 1 BBa_I712003 range1952285 1 697 1890 BBa_I712004 1 BBa_I712004 CMV promoter 2007-10-18T11:00:00Z 2015-08-31T04:07:45Z pcDNA3 by Invitrogen a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included. false false _130_ 0 1896 9 It's complicated false Cloned into pSB1AC3 vector. false Andrej Ondracka BBa_I712006_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattactagatggagcagaagctgatcagcgaggaggactactagatggattatcaagtgtcaagtccaatctatgacatcaattattatacatcggagccctgccaaaaaatcaatgtgaagcaaatcgcagcccgcctcctgcctccgctctactcactggtgttcatctttggttttgtgggcaacatgctggtcatcctcatcctgataaactgcaaaaggctgaagagcatgactgacatctacctgctcaacctggccatctctgacctgtttttccttcttactgtccccttctgggctcactatgctgccgcccagtgggactttggaaatacaatgtgtcaactcttgacagggctctattttataggcttcttctctggaatcttcttcatcatcctcctgacaatcgataggtacctggctgtcgtccatgctgtgtttgctttaaaagccaggacggtcacctttggggtggtgacaagtgtgatcacttgggtggtggctgtgtttgcgtctctcccaggaatcatctttaccagatctcaaaaagaaggtcttcattacacttgcagctctcattttccatacagtcagtatcaattctggaagaatttccagacattaaagatagtcatcttggggctggtcctgccgctgcttgtcatggtcatctgctactcgggaatcctaaaaactctgcttcggtgtcgaaatgagaagaagaggcacagggctgtgaggcttatcttcaccatcatgattgtttattttctcttctgggctccctacaacattgtccttctcctgaacaccttccaggagttctttggcctgaataattgcagtagctctaacaggttggaccaagctatgcaggtgacagagactcttgggatgacgcactgctgcatcaaccccatcatctatgcctttgtcggggagaagttcagaaactacctcttagtcttcttccaaaagcacattgccaaacgcttctgcaaatgctgttctattttccagcaagaggctcccgagcgagcaagctcagtttacacccgatccactggggagcaggaaatatctgtgggcttgatgcagattttcgtcaagactttgaccggtaaaaccggaacattggaagttgaatcttccgataccatcgacaacgttaagtcgaaaattcaagacaaggaaggaatccctggtggatgtcgacctcgagtcatgtaa BBa_I712004_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaat BBa_I712003_sequence 1 atggattatcaagtgtcaagtccaatctatgacatcaattattatacatcggagccctgccaaaaaatcaatgtgaagcaaatcgcagcccgcctcctgcctccgctctactcactggtgttcatctttggttttgtgggcaacatgctggtcatcctcatcctgataaactgcaaaaggctgaagagcatgactgacatctacctgctcaacctggccatctctgacctgtttttccttcttactgtccccttctgggctcactatgctgccgcccagtgggactttggaaatacaatgtgtcaactcttgacagggctctattttataggcttcttctctggaatcttcttcatcatcctcctgacaatcgataggtacctggctgtcgtccatgctgtgtttgctttaaaagccaggacggtcacctttggggtggtgacaagtgtgatcacttgggtggtggctgtgtttgcgtctctcccaggaatcatctttaccagatctcaaaaagaaggtcttcattacacttgcagctctcattttccatacagtcagtatcaattctggaagaatttccagacattaaagatagtcatcttggggctggtcctgccgctgcttgtcatggtcatctgctactcgggaatcctaaaaactctgcttcggtgtcgaaatgagaagaagaggcacagggctgtgaggcttatcttcaccatcatgattgtttattttctcttctgggctccctacaacattgtccttctcctgaacaccttccaggagttctttggcctgaataattgcagtagctctaacaggttggaccaagctatgcaggtgacagagactcttgggatgacgcactgctgcatcaaccccatcatctatgcctttgtcggggagaagttcagaaactacctcttagtcttcttccaaaagcacattgccaaacgcttctgcaaatgctgttctattttccagcaagaggctcccgagcgagcaagctcagtttacacccgatccactggggagcaggaaatatctgtgggcttgatgcagattttcgtcaagactttgaccggtaaaaccggaacattggaagttgaatcttccgataccatcgacaacgttaagtcgaaaattcaagacaaggaaggaatccctggtggatgtcgacctcgagtcatgtaa BBa_I712007_sequence 1 atggagcagaagctgatcagcgaggaggac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z