BBa_I712028 1 BBa_I712028 CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence 2007-10-20T11:00:00Z 2015-08-31T04:07:46Z From one clone that Gabi gave me - will be further described in the future. This protein is used to show that T7 polymerase is actually going into the nucleolus, where it can transcribe genes under T7 promotor. false false _130_ 0 1835 9 It's complicated false In the original cherry protein there was a PstI restriction site in the middle of the sequence. That is why mutagenesis of this restiction site was necessary. true Marko Bitenc BBa_I712016 1 BBa_I712016 myristoilation signal sequence fused to Tobacco Etch Virus (TEV) protease cleavage site 2007-10-22T11:00:00Z 2015-08-31T04:07:46Z Signal sequence for myristoilation with proteolytic cleavage site for TEV protease. false false _130_ 0 1883 9 In stock false true Katja Kolar BBa_I712055 1 BBa_I712055 myri - TEVP - mCherry - NLS 2007-10-23T11:00:00Z 2015-08-31T04:07:46Z Signal sequence for myristoilation fused with proteolytic cleavage site for TEV protease and mCherry with NLS. false false _130_ 0 1883 9 It's complicated false false Katja Kolar component1953722 1 BBa_I712016 component1953723 1 BBa_I712028 annotation1953722 1 BBa_I712016 range1953722 1 1 57 annotation1953723 1 BBa_I712028 range1953723 1 64 796 BBa_I712055_sequence 1 atgggcatcggcaagtccaagagctccgccagctccgagaacctgtacttccagggctactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctccaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggatccaaaaaagaagagaaaggtaa BBa_I712028_sequence 1 atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctccaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggatccaaaaaagaagagaaaggtaa BBa_I712016_sequence 1 atgggcatcggcaagtccaagagctccgccagctccgagaacctgtacttccagggc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z