BBa_I712078 1 BBa_I712078 TEV protease (C-terminal half) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z Tobacco Etch Virus C-terminal half of TEV (Tobacco Etch Virus) protease. TEV protease is a highly site-specific protease that is found in the Tobacco Etch Virus (TEV). One of the main uses of this protease is for removing affinity tags from purified proteins. But scientists developed assay for detecting transmembrane protein interactions on its base. TEV protease is splitted into halves (N-terminal and C-terminal). These halves are inactive but assembles and regain activity if they are fused with interacting transmembrane proteins. For detecting TEV protease activity another fusion protein is constructed which is composed of transmembrane region, specific TEV protease cleavage site and kind of transcription factor. When both halves of TEV protease assembles and therefore split protease regains activity it cleaves third protein and transcription factor is released from membrane. Transcription factor is later translocated to the nucleus where induces transcription of promoter with reporter gene. This is C-terminal half of this protease. false false _130_ 0 1856 9 It's complicated false Synthetic gene true Rok Gaber annotation1952300 1 STOP range1952300 1 373 375 annotation1952299 1 Coding range1952299 1 1 372 BBa_I712078_sequence 1 aagagcatgtctagcatggtgtcagacaccagttgcacattcccttcatctgatggcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagtattgtgggggggccataaagttttcatgagcaaacctgaagagccttttcagccagttaaggaagcgactcaactcatgagtgaattggtgtactcgcaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z