BBa_I712077 1 TEV TEV protease (N-terminal half) - entered sequence may be wrong 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z Tobacco Etch Virus N-terminal half of TEV (Tobacco Etch Virus) protease. TEV protease is a highly site-specific protease that is found in the Tobacco Etch Virus (TEV). One of the main uses of this protease is for removing affinity tags from purified proteins. But scientists developed assay for detecting transmembrane protein interactions on its base. TEV protease is splitted into halves (N-terminal and C-terminal). These halves are inactive but assembles and regain activity if they are fused with interacting transmembrane proteins. For detecting TEV protease activity another fusion protein is constructed which is composed of transmembrane region, specific TEV protease cleavage site and kind of transcription factor. When both halves of TEV protease assembles and therefore split protease regains activity it cleaves third protein and transcription factor is released from membrane. Transcription factor is later translocated to the nucleus where induces transcription of promoter with reporter gene. This is N-terminal half of this protease. false false _130_ 0 1856 9 It's complicated false Synthetic gene true Rok Gaber annotation1952295 1 Coding range1952295 1 1 354 annotation1952296 1 STOP range1952296 1 355 357 BBa_I712076 1 BBa_I712076 CMV promoter - Myc tag - CCR5 (shortened) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z Cytomegalovirus, Human genome Human CCR5 receptor under control of CMV promoter. Human CCR5 transmembrane receptor is a chemokine receptor which plays cruical role in HIV infection. This version of CCR5 is shortened for 50 AK from C-terminus, therefore is without cytosolic domain. Myc tag is fused to the N-terminal of this protein. false false _130_ 0 1856 9 In stock false true Rok Gaber annotation1952473 1 Myc tag range1952473 1 656 685 annotation1952471 1 CCR5 (shortened) range1952471 1 685 1608 annotation1952472 1 CMV promoter range1952472 1 1 655 BBa_I712079 1 BBa_I712079 CMV promoter - Myc tag - CCR5 (shortened) - TEV N 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z Cytomegalovirus, Tobacco Etch Virus, Human Human CCR5 shortened receptor fused with N-terminal half of TEV protease and Myc tag. This construct is under control of CMV promoter. Human CCR5 transmembrane receptor is a chemokine receptor which plays cruical role in HIV infection. This version of CCR5 is shortened for 50 AK from C-terminus, therefore is without cytosolic domain. Myc tag is fused to the N-terminal of this protein. CCR5 (shortened) is also fused with N-terminal half of TEV protease. This construct is coexpressed with CD4 receptor fused with C-terminal half of TEV protease (in mammalian cells). When that kind of human cells are infected with HIV virus both halves of TEV protease assemble and split protein regains its function. This principle can be used for fighting HIV infection. false false _130_ 0 1856 9 It's complicated true true Rok Gaber component1952485 1 BBa_I712077 component1952482 1 BBa_I712076 annotation1952482 1 BBa_I712076 range1952482 1 1 1608 annotation1952485 1 BBa_I712077 range1952485 1 1617 1973 BBa_I712076_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaatactagaatggagcagaagctgatcagcgaggaggacactagaatggattatcaagtgtcaagtccaatctatgacatcaattattatacatcggagccctgccaaaaaatcaatgtgaagcaaatcgcagcccgcctcctgcctccgctctactcactggtgttcatctttggttttgtgggcaacatgctggtcatcctcatcctgataaactgcaaaaggctgaagagcatgactgacatctacctgctcaacctggccatctctgacctgtttttccttcttactgtccccttctgggctcactatgctgccgcccagtgggactttggaaatacaatgtgtcaactcttgacagggctctattttataggcttcttctctggaatcttcttcatcatcctcctgacaatcgataggtacctggctgtcgtccatgctgtgtttgctttaaaagccaggacggtcacctttggggtggtgacaagtgtgatcacttgggtggtggctgtgtttgcgtctctcccaggaatcatctttaccagatctcaaaaagaaggtcttcattacacttgcagctctcattttccatacagtcagtatcaattctggaagaatttccagacattaaagatagtcatcttggggctggtcctgccgctgcttgtcatggtcatctgctactcgggaatcctaaaaactctgcttcggtgtcgaaatgagaagaagaggcacagggctgtgaggcttatcttcaccatcatgattgtttattttctcttctgggctccctacaacattgtccttctcctgaacaccttccaggagttctttggcctgaataattgcagtagctctaacaggttggaccaagctatgcaggtgacagagactcttgggatgacgcactgctgcatcaaccccatcatctatgcctttgtcggggagaagttc BBa_I712079_sequence 1 cgatgtacgggccagatatacgcgttgacattgattattgcctagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaatactagaatggagcagaagctgatcagcgaggaggacactagaatggattatcaagtgtcaagtccaatctatgacatcaattattatacatcggagccctgccaaaaaatcaatgtgaagcaaatcgcagcccgcctcctgcctccgctctactcactggtgttcatctttggttttgtgggcaacatgctggtcatcctcatcctgataaactgcaaaaggctgaagagcatgactgacatctacctgctcaacctggccatctctgacctgtttttccttcttactgtccccttctgggctcactatgctgccgcccagtgggactttggaaatacaatgtgtcaactcttgacagggctctattttataggcttcttctctggaatcttcttcatcatcctcctgacaatcgataggtacctggctgtcgtccatgctgtgtttgctttaaaagccaggacggtcacctttggggtggtgacaagtgtgatcacttgggtggtggctgtgtttgcgtctctcccaggaatcatctttaccagatctcaaaaagaaggtcttcattacacttgcagctctcattttccatacagtcagtatcaattctggaagaatttccagacattaaagatagtcatcttggggctggtcctgccgctgcttgtcatggtcatctgctactcgggaatcctaaaaactctgcttcggtgtcgaaatgagaagaagaggcacagggctgtgaggcttatcttcaccatcatgattgtttattttctcttctgggctccctacaacattgtccttctcctgaacaccttccaggagttctttggcctgaataattgcagtagctctaacaggttggaccaagctatgcaggtgacagagactcttgggatgacgcactgctgcatcaaccccatcatctatgcctttgtcggggagaagttctactagagggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcacttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaacttaa BBa_I712077_sequence 1 ggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcacttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaacttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z