LOCUS BBa_I713001 4177 bp DNA linear 25-Nov-2024 DEFINITION A pBBR1MCS plasmid without the mob gene. ACCESSION BBa_I713001 VERSION BBa_I713001.1 FEATURES Location/Qualifiers misc_feature 1..6 /label=SpeI misc_feature 19..24 /label=PstI promoter 90..109 /label=T3 promoter promoter 179..208 /label=lac promoter CDS 301..990 /label=chloramphenicol acetyltransferase misc_feature 360..990 /label=CAT marker CDS 1499..2125 /label=Mob CDS 2714..3388 /label=replication protein misc_feature 3955..4113 /label=lacZalpha reporter promoter 4121..4139 /label=T7 promoter misc_feature 4172..4177 /label=XbaI misc_feature 4164..4171 /label=NotI misc_feature 25..30 /label=EcoRI misc_feature 772..777 /label=EcoRI ORIGIN 1 actagtggat cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc 61 gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc 121 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 181 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 241 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 301 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgca tgcataaaaa 361 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac 421 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa 481 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac 541 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag 601 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc 661 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca 721 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg 781 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt 841 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca 901 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac 961 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga 1021 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct 1081 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac 1141 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcgaa 1201 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1261 cttagacgtc aggtggcact tttcggggaa atgtgcgcgc ccgcgttcct gctggcgctg 1321 ggcctgtttc tggcgctgga cttcccgctg ttccgtcagc agcttttcgc ccacggcctt 1381 gatgatcgcg gcggccttgg cctgcatatc ccgattcaac ggccccaggg cgtccagaac 1441 gggcttcagg cgctcccgaa ggtctcgggc cgtctcttgg gatgctagct attaattgct 1501 agctgacgta ctcgaccgcc aacacagcgt ccttgcgccg cttctctggc agcaactcgc 1561 gcagtcggcc catcgcttca tcggtgctgc tggccgccca gtgctcgttc tctggcgtcc 1621 tgctggcgtc agcgttgggc gtctcgcgct cgcggtaggc gtgcttgaga ctggccgcca 1681 cgttgcccat tttcgccagc ttcttgcatc gcatgatcgc gtatgccgcc atgcctgccc 1741 ctcccttttg gtgtccaacc ggctcgacgg gggcagcgca aggcggtgcc tccggcgggc 1801 cactcaatgc ttgagtatac tcactagact ttgcttcgca aagtcgtgac cgcctacggc 1861 ggctgcggcg ccctacgggc ttgctctccg ggcttcgccc tgcgcggtcg ctgcgctccc 1921 ttgccagccc gtggatatgt ggacgatggc cgcgagcggc caccggctgg ctcgcttcgc 1981 tcggcccgtg gacaaccctg ctggacaagc tgatggacag gctgcgcctg cccacgagct 2041 tgaccacagg gattgcccac cggctaccca gccttcgacc acatacccac cggctccaac 2101 tgcgcggcct gcggccttgc cccatcaatt tttttaattt tctctgggga aaagcctccg 2161 gcctgcggcc tgcgcgcttc gcttgccggt tggacaccaa gtggaaggcg ggtcaaggct 2221 cgcgcagcga ccgcgcagcg gcttggcctt gacgcgcctg gaacgaccca agcctatgcg 2281 agtgggggca gtcgaaggcg aagcccgccc gcctgccccc cgagcctcac ggcggcgagt 2341 gcgggggttc caagggggca gcgccacctt gggcaaggcc gaaggccgcg cagtcgatca 2401 acaagccccg gaggggccac tttttgccgg agggggagcc gcgccgaagg cgtgggggaa 2461 ccccgcaggg gtgcccttct ttgggcacca aagaactaga tatagggcga aatgcgaaag 2521 acttaaaaat caacaactta aaaaaggggg gtacgcaaca gctcattgcg gcaccccccg 2581 caatagctca ttgcgtaggt taaagaaaat ctgtaattga ctgccacttt tacgcaacgc 2641 ataattgttg tcgcgctgcc gaaaagttgc agctgattgc gcatggtgcc gcaaccgtgc 2701 ggcaccctac cgcatggaga taagcatggc cacgcagtcc agagaaatcg gcattcaagc 2761 caagaacaag cccggtcact gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg 2821 gcttattgcg aggaaaccca cggcggcaat gctgctgcat cacctcgtgg cgcagatggg 2881 ccaccagaac gccgtggtgg tcagccagaa gacactttcc aagctcatcg gacgttcttt 2941 gcggacggtc caatacgcag tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa 3001 gctcaacggc cccggcaccg tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca 3061 gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga 3121 ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc atcccgaccc tgtatccggg 3181 cgagcagcaa ctaccgaccg gccccggcga ggagccgccc agccagcccg gcattccggg 3241 catggaacca gacctgccag ccttgaccga aacggaggaa tgggaacggc gcgggcagca 3301 gcgcctgccg atgcccgatg agccgtgttt tctggacgat ggcgagccgt tggagccgcc 3361 gacacgggtc acgctgccgc gccggtagca cttgggttgc gcagcaaccc gtaagtgcgc 3421 tgttccagac tatcggctgt agccgcctcg ccgccctata ccttgtctgc ctccccgcgt 3481 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc 3541 taacggattc accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat 3601 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt 3661 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta 3721 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc 3781 cgcttattat cacttattca ggcgtagcac caggcgttta agggcaccaa taactgcctt 3841 aaaaaaatta cgccccgccc tgccactcat cgcagtcggc ctattggtta aaaaatgagc 3901 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc 3961 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 4021 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 4081 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg 4141 cgaattggag ctccaccgcg gtggcggccg ctctaga //