LOCUS BBa_I713007 6593 bp DNA linear 22-Nov-2024 DEFINITION pEB T7X / Amp Kan ACCESSION BBa_I713007 VERSION BBa_I713007.1 FEATURES Location/Qualifiers CDS 3981..4775 /label=kan CDS 4793..5653 /label=amp CDS 300..2954 /label=T7 RNA Polymerase gene without ATG ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattgcc cttggatcct 301 tacgcgaacg cgaagtccga ctctaagatg tcacggaggt tcaagttacc tttagccgga 361 agtgctggca ttttgtccaa ttgagactcg tgcaactggt cagcgaactg gtcgtagaaa 421 tcagccagta catcacaaga ctcatatgtg tcaaccatag tttcgcgcac tgctttgaac 481 aggttcgcag cgtcagccgg aatggtaccg aaggagtcgt gaatcagtgc aaaagattcg 541 attccgtact tctcgtgtgc ccacactaca gtcttacgaa ggtggctacc gtcttggctg 601 tgtacaaagt taggagcgat accagactcc tgtttgtgtg catcaatctc gctatctttg 661 ttggtgttaa tggtaggctg taagcggaac tgaccgagga acatcaggtt caagcgcgtc 721 tgaataggct tcttgtattc ctgccacaca gggaaaccat caggagttac ccaatgcaca 781 gcgcaacgct tgcgaagaat ctctccagtc ttcttatctt tgacctcagc agccagcagc 841 ttagcagcag acttaagcca gttcattgct tcaaccgcag ctaccaccgt cacgctcaca 901 gattcccaaa tcagcttagc catgtatcca gcagcctgat tcggctgagt gaacatcaga 961 cccttgccgg aatcaatagc tggctgaatg gtatcttcca gcacttgttg acggaagccg 1021 aactctttgg acccgtaagc cagcgtcatg actgaacgct tagtcacact gcgagtaaca 1081 ccgtaagcca gccattgacc agccagtgcc ttagtgccca gcttgacttt ctcagagatt 1141 tcaccagtgt tctcatcggt cacggtaact acttcgttat cggtcccatt gattgcgtct 1201 gcttgtagaa tctcgttgac tttcttagca acaatcccgt agatgtcctg aacggtttca 1261 ctaggaagca agttaaccgc gcgaccacct acctcatctc ggagcatcgc ggagaagtgc 1321 tggatgccag agcaagaccc gtcaaacgcc agcggaaggg agcagttata gctcaggccg 1381 tggtgctgta ccccagcgta ctcaaagcag aacgcaagga agcagaacgg agaatcttgc 1441 tcagcccacc aagtgttctc cagtggagac ttagcgcaag ccatgatgtt ctcgtggttt 1501 tcctcaatga acttgatgcg ctcagggaac ggaaccttat cgacacccgc acagtttgca 1561 ccgtggattt tcagccagta gtaaccttcc ttaccgattg gtttaccttt cgccagcgta 1621 agcagtcctt tggtcatatc gttaccttgc gggttgaaca ttgacacagc gtaaacacga 1681 ccgcgccagt ccatgttgta agggaaccag atggccttat ggttagcaaa cttattggct 1741 tgctcaagca tgaactcaag gctgatacgg cgagacttgc gagccttgtc cttgcggtac 1801 acagcagcgg cagcacgttt ccacgcggtg agagcctcag gattcatgtc gatgtcttcc 1861 ggtttcatcg ggagttcttc acgctcaatc gcagggatgt cctcgaccgg acaatgcttc 1921 cacttggtga ttacgttggc gaccgctagg actttcttgt tgattttcca tgcggtgttt 1981 tgcgcaatgt taatcgcttt gtacacctca ggcatgtaaa cgtcttcgta gcgcatcagt 2041 gctttcttac tgtgagtacg caccagcgcc agaggacgac gaccgttagc ccaatagcca 2101 ccaccagtaa tgccagtcca cggcttagga ggaactacgc aaggttggaa catcggagag 2161 atgccagcca gcgcacctgc acgggttgcg atagcctcag cgtattcagg tgcgagttcg 2221 atagtctcag agtcttgacc tactacgcca gcattttggc ggtgtaagct aaccattccg 2281 gttgactcaa tgagcatctc gatgcagcgt actcctacat gaatagagtc ttccttatgc 2341 cacgaagacc acgcctcgcc accgagtaga cccttagaga gcatgtcagc ctcgacaact 2401 tgcataaatg ctttcttgta gacgtgccct acgcgcttgt tgagttgttc ctcaacgttt 2461 ttcttgaagt gcttagcttc aaggtcacgg atacgaccga agcgagcctc gtcctcaatg 2521 gcccgaccga ttgcgcttgc tacagcctga acggttgtat tgtcagcact ggttaggcaa 2581 gccagagtgg tcttaatggt gatgtacgct acggcttccg gcttgatttc ttgcaggaac 2641 tggaaggctg tcgggcgctt gccgcgctta gctttcactt cctcaaacca gtcgttgatg 2701 cgtgcaatca tcttagggag tagggtagtg atgagaggct tggcggcagc gttatccgca 2761 acctcaccag ctttaagttg acgctcaaac atcttgcgga agcgtgcttc acccatctcg 2821 taagactcat gctcaagggc caactgttcg cgagctaaac gctcaccgta atggtcagcc 2881 agagtgttga acgggatagc agccagttcg atgtcagaga agtcgttctt agcgatgtta 2941 atcgtgttca gactagtaag ggcaattctg cagatatcca tcacactggc ggccgctcga 3001 gcatgcatct agagggccca attcgcccta tagtgagtcg tattacaatt cactggccgt 3061 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 3121 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 3181 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg 3241 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 3301 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 3361 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 3421 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 3481 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 3541 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 3601 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaattca gggcgcaagg 3661 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga 3721 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg 3781 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg 3841 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact 3901 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga 3961 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 4021 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 4081 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 4141 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 4201 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 4261 tgggcgaagt gccggggcag gatctcctgt catcccacct tgctcctgcc gagaaagtat 4321 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 4381 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 4441 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 4501 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 4561 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 4621 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 4681 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 4741 tcgccttcta tcgccttctt gacgagttct tctgaattga aaaaggaaga gtatgagtat 4801 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 4861 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 4921 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 4981 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 5041 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 5101 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 5161 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 5221 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 5281 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 5341 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 5401 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 5461 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 5521 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 5581 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 5641 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 5701 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 5761 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 5821 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 5881 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 5941 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 6001 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 6061 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 6121 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 6181 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 6241 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 6301 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 6361 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 6421 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 6481 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 6541 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag //