LOCUS BBa_I713025 4875 bp DNA linear 22-Nov-2024 DEFINITION pEB-lambda ACCESSION BBa_I713025 VERSION BBa_I713025.1 FEATURES Location/Qualifiers CDS 1333..1490 /label=lacZ misc_feature 366..1204 /label=FRT insert CDS 2263..3057 /label=kan(r) CDS 3075..3935 /label=amp(r) ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattgcc cttaacgtgt 301 tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgag cttcaaaagc 361 gctctgaagt tcctatactt tctagagaat aggaacttcg aactgcaggt cgacggatcc 421 ccggaattaa ttctcatgtt tgacagctta tcactgatca gtgaattaat ggcgatgacg 481 catcctcacg ataatatccg ggtaggcgca atcactttcg tctctactcc gttacaaagc 541 gaggctgggt atttcccggc ctttctgtta tccgaaatcc actgaaagca cagcggctgg 601 ctgaggagat aaataataaa cgaggggctg tatgcacaaa gcatcttctg ttgagttaag 661 aacgagtatc gagatggcac atagccttgc tcaaattgga atcaggtttg tgccaatacc 721 agtagaaaca gacgaagaag ctagctttgc actggattgc gaggctttgc catggctaat 781 tcccatgtca gccgtgcaca tttccccgaa aagtgccacc tgcatcgatg gccccccgat 841 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa 901 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct 961 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg 1021 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac 1081 ggatggcctt tttgcgtggc cagtgccaag cttgcatgca gattgcagca ttacacgtct 1141 tgagcgattg tgtaggctgg agctgcttcg aagttcctat actttctaga gaataggaac 1201 ttcggaatag gaacttcaag atccccttat tacaagaaca agggcaattc tgcagatatc 1261 catcacactg gcggccgctc gagcatgcat ctagagggcc caattcgccc tatagtgagt 1321 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 1381 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 1441 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg 1501 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 1561 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 1621 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 1681 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 1741 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 1801 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 1861 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 1921 taacaaaatt cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg 1981 cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac 2041 gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg 2101 gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt 2161 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg 2221 ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 2281 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 2341 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 2401 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 2461 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 2521 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatcccac 2581 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 2641 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 2701 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 2761 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 2821 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 2881 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 2941 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 3001 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt 3061 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 3121 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 3181 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 3241 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 3301 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 3361 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 3421 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 3481 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 3541 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 3601 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 3661 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 3721 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 3781 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 3841 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 3901 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 3961 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 4021 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 4081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 4141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 4201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 4261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 4321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 4381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 4441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 4501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 4561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 4621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 4681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 4741 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 4801 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 4861 gcgaggaagc ggaag //