LOCUS BBa_I713034 4943 bp DNA linear 22-Nov-2024 DEFINITION pT7Blue-XisT7X ACCESSION BBa_I713034 VERSION BBa_I713034.1 FEATURES Location/Qualifiers CDS 22..138 /label=UmuD marker CDS 2289..4940 /label=T7 RNAP gene without ATG CDS 1291..2151 /label=amp(r) ORIGIN 1 agtagcagca gcggctgcca gatcgatgcg ctgttcaacg taatctgctg ccggtgaagg 61 aaagccacac tgaacaagat cgctaaatag cggaaaagtc acaatttcgc ggagatccgc 121 aggcttgata aactccatgg ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 181 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 241 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 301 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 361 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 421 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 481 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 541 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 601 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 661 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 721 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 781 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 841 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 901 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 961 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 1021 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 1081 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 1141 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 1201 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 1261 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 1321 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 1381 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 1441 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 1501 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 1561 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 1621 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 1681 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 1741 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 1801 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 1861 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 1921 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 1981 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 2041 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 2101 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 2161 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 2221 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 2281 tgggatcctt acgcgaacgc gaagtccgac tctaagatgt cacggaggtt caagttacct 2341 ttagccggaa gtgctggcat tttgtccaat tgagactcgt gcaactggtc agcgaactgg 2401 tcgtagaaat cagccagtac atcacaagac tcatatgtgt caaccatagt ttcgcgcact 2461 gctttgaaca ggttcgcagc gtcagccgga atggtaccga aggagtcgtg aatcagtgca 2521 aaagattcga ttccgtactt ctcgtgtgcc cacactacag tcttacgaag gtggctaccg 2581 tcttggctgt gtacaaagtt aggagcgata ccagactcct gtttgtgtgc atcaatctcg 2641 ctatctttgt tggtgttaat ggtaggctgt aagcggaact gaccgaggaa catcaggttc 2701 aagcgcgtct gaataggctt cttgtattcc tgccacacag ggaaaccatc aggagttacc 2761 caatgcacag cgcaacgctt gcgaagaatc tctccagtct tcttatcttt gacctcagca 2821 gccagcagct tagcagcaga cttaagccag ttcattgctt caaccgcagc taccaccgtc 2881 acgctcacag attcccaaat cagcttagcc atgtatccag cagcctgatt cggctgagtg 2941 aacatcagac ccttgccgga atcaatagct ggctgaatgg tatcttccag cacttgttga 3001 cggaagccga actctttgga cccgtaagcc agcgtcatga ctgaacgctt agtcacactg 3061 cgagtaacac cgtaagccag ccattgacca gccagtgcct tagtgcccag cttgactttc 3121 tcagagattt caccagtgtt ctcatcggtc acggtaacta cttcgttatc ggtcccattg 3181 attgcgtctg cttgtagaat ctcgttgact ttcttagcaa caatcccgta gatgtcctga 3241 acggtttcac taggaagcaa gttaaccgcg cgaccaccta cctcatctcg gagcatcgcg 3301 gagaagtgct ggatgccaga gcaagacccg tcaaacgcca gcggaaggga gcagttatag 3361 ctcaggccgt ggtgctgtac cccagcgtac tcaaagcaga acgcaaggaa gcagaacgga 3421 gaatcttgct cagcccacca agtgttctcc agtggagact tagcgcaagc catgatgttc 3481 tcgtggtttt cctcaatgaa cttgatgcgc tcagggaacg gaaccttatc gacacccgca 3541 cagtttgcac cgtggatttt cagccagtag taaccttcct taccgattgg tttacctttc 3601 gccagcgtaa gcagtccttt ggtcatatcg ttaccttgcg ggttgaacat tgacacagcg 3661 taaacacgac cgcgccagtc catgttgtaa gggaaccaga tggccttatg gttagcaaac 3721 ttattggctt gctcaagcat gaactcaagg ctgatacggc gagacttgcg agccttgtcc 3781 ttgcggtaca cagcagcggc agcacgtttc cacgcggtga gagcctcagg attcatgtcg 3841 atgtcttccg gtttcatcgg gagttcttca cgctcaatcg cagggatgtc ctcgaccgga 3901 caatgcttcc acttggtgat tacgttggcg accgctagga ctttcttgtt gattttccat 3961 gcggtgtttt gcgcaatgtt aatcgctttg tacacctcag gcatgtaaac gtcttcgtag 4021 cgcatcagtg ctttcttact gtgagtacgc accagcgcca gaggacgacg accgttagcc 4081 caatagccac caccagtaat gccagtccac ggcttaggag gaactacgca aggttggaac 4141 atcggagaga tgccagccag cgcacctgca cgggttgcga tagcctcagc gtattcaggt 4201 gcgagttcga tagtctcaga gtcttgacct actacgccag cattttggcg gtgtaagcta 4261 accattccgg ttgactcaat gagcatctcg atgcagcgta ctcctacatg aatagagtct 4321 tccttatgcc acgaagacca cgcctcgcca ccgagtagac ccttagagag catgtcagcc 4381 tcgacaactt gcataaatgc tttcttgtag acgtgcccta cgcgcttgtt gagttgttcc 4441 tcaacgtttt tcttgaagtg cttagcttca aggtcacgga tacgaccgaa gcgagcctcg 4501 tcctcaatgg cccgaccgat tgcgcttgct acagcctgaa cggttgtatt gtcagcactg 4561 gttaggcaag ccagagtggt cttaatggtg atgtacgcta cggcttccgg cttgatttct 4621 tgcaggaact ggaaggctgt cgggcgcttg ccgcgcttag ctttcacttc ctcaaaccag 4681 tcgttgatgc gtgcaatcat cttagggagt agggtagtga tgagaggctt ggcggcagcg 4741 ttatccgcaa cctcaccagc tttaagttga cgctcaaaca tcttgcggaa gcgtgcttca 4801 cccatctcgt aagactcatg ctcaagggcc aactgttcgc gagctaaacg ctcaccgtaa 4861 tggtcagcca gagtgttgaa cgggatagca gccagttcga tgtcagagaa gtcgttctta 4921 gcgatgttaa tcgtgttcag act //