LOCUS BBa_I713036 4741 bp DNA linear 22-Dec-2024 DEFINITION pET15b-LacI(-) ACCESSION BBa_I713036 VERSION BBa_I713036.1 FEATURES Location/Qualifiers CDS 756..978 /label=disrupted lacI CDS 3676..4533 /label=amp(r) ORIGIN 1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 301 cgggctttgt tagcagccgg atcctcgagc atatgacgac cttcgatatg gccgctgctg 361 tgatgatgat gatgatgatg atgatgatgg cccatggtat atctccttct taaagttaaa 421 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat 481 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc 541 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 601 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 661 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 721 aacggcctca acctactact gggctgcttc ctaataacca ccatcaaaca ggattttcgc 781 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 841 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 901 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 961 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc 1021 accgggatct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc 1081 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg 1141 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac 1201 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt 1261 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc 1321 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat 1381 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag 1441 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct 1501 aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg 1561 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg 1621 tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac 1681 ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa 1741 accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc 1801 atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg 1861 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag 1921 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc 1981 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg 2041 ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta 2101 acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc 2161 agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc 2221 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac 2281 acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga 2341 agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac 2401 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc 2461 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 2521 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 2581 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg 2641 cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag 2701 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 2761 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 2821 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 2881 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 2941 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 3001 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 3061 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 3121 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 3181 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 3241 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 3301 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 3361 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 3421 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 3481 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 3541 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 3601 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 3661 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 3721 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 3781 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 3841 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 3901 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 3961 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg 4021 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 4081 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 4141 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 4201 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 4261 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 4321 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 4381 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 4441 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 4501 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 4561 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 4621 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 4681 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga 4741 a //