BBa_I714036
1
BBa_I714036
Riboregulator key 1
2007-10-24T11:00:00Z
2015-08-31T04:07:47Z
J01008
Released HQ 2013
The same as J01008
false
false
_142_
0
1911
9
In stock
false
The Isaacs key1 riboregulator taR12 is modeled on the hok/sok post-segregational killing method of plasmid R1. It makes 25 base pairs with its counterpart, the riboregulator J01010 (aka "lock 1" or "crR12").
Isaacs documentation (see reference #1):
Upon induction of the araC promoter (using 0.25% arabinose solution), the taR12 key activated the corresponding crR12 lock to express the downstream indicator, GFP, to levels of fluorescence 19-fold greater than without taR12 expression. Maximal expression was reached at 70 minutes after addition of arabinose solution, and was maintained after that point.
false
Chunbo Lou
BBa_I714036_sequence
1
acccaaatccaggaggtgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z