BBa_I714038 1 BBa_I714038 pSC101 with relaxase and oriT deleted 2007-10-25T11:00:00Z 2015-08-31T04:07:47Z plasmid pSC101. Genebank NC_002056. This is a conjugative plasmid pSC101 with mobA (relaxase coding gene) and oriT (origin of transfer) knocked out, providing all other necessary proteins for conjugative transfer of plasmid pSC101 in the help of conjugative plasmid R751. The plasmid is a mutant, unable to perform conjugative functions, but when pSC101 MobA protein is supplied in the R751 strain, any plasmid with pSC101 oriT transfer origin in this chasis can be conjugatively transfered into recipient cells. false false _142_ 0 2055 9 Not in stock false Region of deletion: 2936bp - 4115bp (containing mobA and oriT) and substituted with PstI restriction site CTGCAG Method of deletion: in vitro reversed cyclic PCR and self-ligation. false Anting Xu BBa_I714038_sequence 1 ttctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccactccaagaattggagccaatcaattcttgcggagaactgtgaatgcgcaaaccaacccttggcagaacatatccatcgcgtccgccatctccagcagccgcacgcggcgcatctcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctggaaacgcggaagtcccctacgtgctgctgaagttgcccgcaacagagagtggaaccaaccggtgataccacgatactatgactgagagtcaacgccatgagcggcctcatttcttattctgagttacaacagtccgcaccgctgccggtagctacttgactatccggctgcactagccctgcgtcagatggctctgatccaaggcaaactgccaaaatatctgctggcaccggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggaacacctacatctgtattaacgaagcgctggcattgaccctgagtgatttttctctggtgccgccctatccctttgtgcagcttgccacgctcaaaggggtttgaggtccaaccgtacgaaaacgtacggtaagaggaaaattatcgtctgaaaaatcgattagtagacaagaaagtccgttaagtgccaattttcgattaaaaagacaccgttttgatggcgttttccaatgtacattatgtttcgatatatcagacagttacttcactaacgtacgttttcgttctattggccttcagaccccatatccttaatgtcctttatttgctggggttatcagatccccccgacacgtttaattaatgctttctccgccggagatcgacgcacaggcttctgtgtctatgatgttatttcttaataatcatccaggtattctctttatcaccatacgtagtgcgagtgtccaccttaacgcagggctttccgtcacagcgcgatatgtcagccagcggggctttcttttgccagaccgcttccatcctctgcatttcagcaatctggctatacccgtcattcataaaccacgtaaatgccgtcacgcaggaagccaggacgaagaatatcgtcagtacaagataaatcgcggatttccacgtatagcgtgacatctcacgacgcatttcatggatcatcgctttcgccgtatcggcagcctgattcagcgcttctgtcgccggtttctgctgtgctaatccggcttgtttcagttctttctcaacctgagtgagcgcggaactcaccgatttcctgacggtgtcagtcatattaccggacgcgctgtccagctcacgaatgaccctgctcagcgtttcactttgctgctgtaattgtgatgaggcggcctgaaactgttctgtcagagaagtaacacgcttttccagcgcctgatgatgcccgataagggcggcaatttgtttaatttcgtcgctcatacaaaatcctgcctgcaggataaataaaagagttatcagggattgtagtgggatttgacctcctctgccatcatgagcgtaatcattccgttagcattcaggaggtaaacagcatgaataaaagcgaaaaaacaggaacaatgggcagcagaaagagtgcagtatattcgcggcttaaagtcgccgaatgagcaacagaaacttatgctgatactgacggataaagcagataaaacagcacaggatatcaaaacgctgtccctgctgatgaaggctgaacaggcagcagagaaagcgcaggaagccagagcgaaagtcatgaacctgatacaggcagaaaagcgagccgaagccagagccgcccgtaaagcccgtgaccatgctctgtaccagtctgccggattgcttatcctggcgggtctggttgacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggactagtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacgtaacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagcatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttagatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcatttaaagctgttcaccatgaacagatcgacaatgtaacagatgaacagcatgtaacacctaatagaacaggtgaaaccagtaaaacaaagcaactagaacatgaaattgaacacctgagacaacttgttacagctcaacagtcacacatagacagcctgaaacaggcgatgctgcttatcgaatcaaagctgccgacaacacgggagccagtgacgcctcccgtggggaaaaaatcatggcaattctggaagaaatagcgctttcagccggcaaacctgaagccggatctgcgattctgataacaaactagcaacaccagaacagcccgtttgcgggcagcaaaacccgtacttttggacgttccggcggttttttgtggcgagtggtgttcgggcggtgcgcgcaagatccattatgttaaacgggcgagtttacatctcaaaaccgcccgcttaacaccatcagaaatcctcagcgcgattttaagcaccaacccccccccgtaacacccaaatccatactgaaagtggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgcatcctcccgacaacacagaccattccgtggcaaagcaaaagttcagaatcaccaactggtccacctacaacaaagctctcatcaaccgtggctccctcactttctggctggatgatgaggcgattcaggcctggtatgagtcggcaacaccttcatcacgaggaaggccccagcgctattctgatctcgccatcaccaccgttctggtgattaaacgcgtattccggctgaccctgcgggctgcgcagggttttattgattccatttttgccctgatgaacgttccgttgcgctgcccggattacaccagtgtcagtaagcgggcaaagtcggttaatgtcagtttcaaaacgtccacccggggtgaaatcgcacacctggtgattgattccaccgggctgaaggtctttggtgaaggcgaatggaaagtcagaaagcacggcaaagagcgccgtcgtatctggcgaaagttgcatcttgctgttgacagcaacacacatgaagttgtctgtgcagacctgtcgctgaataacgtcacggactcagaagccttcccgggccttatccggcagactcacagaaaaatcagggcagccgcggcagacggggcttacgatacccggctctgtcacgatgaactgcgccgcaaaaaaatcagcgcgcttattcctccccgaaaaggtgcgggttactggcccggtgaatatgcagaccgtaaccgtgcagtggctaatcagcgaatgaccgggagtaatgcgcggtggaaatggacaacagattacaaccgtcgctcgatagcggaaacggcgatgtaccgggtaaaacagctgttcgggggttcactgacgctgcgtgactacgatggtcaggttgcggaggctatggccctggtacgagcgctgaacaaaatgacgaaagcaggtatgcctgaaagcgtgcgtattgcctgaaaacacaacccgctacgggggagacttacccgaaatctgatttattcaacaaagccgggtgtggtgaactacaaagcagacccgttgaggttatcagttcgatgcacaatcagcagcgcataaaatatgcacaagaacaggagcacccttcgcattaagctgtggtggtaacaagtagtgccgggctaccatcagcgagcatgatgcgctcccacagcattcgccttggcagtatggaagttcctcgctccagttcgggccggtatccacctcgagtgaccccagccgcccctcatgccaaccgagcccattaagcgcagagtcggccgcaactatctcggggacgcataaacacgcagcgatttaccaaggagttcggctaggtcaatccgcaattccctttgcctggccggagatgccgcgagcaaacaatgtgtccggtcgctaccagatggactttgtgggcggaggattgcaccagacgcttcagcgcagcgtcgtacgatgaatcggaaggcccggtaagcaattgaagaaccgctgaccacctcccatccgacaatgcaagggtgccccgctcccagcgcgacacggttgcctgatccacacccattaattcagctaggtgactttgcttcatatgacgaagcaaccgcgccctccgcacctgccgccccctgtcattcgtcaacattcttcagcacctcaatgtcgttcgtagtgagcctcattttttcaagaccgccgatgatgagagccaggcctcgctcgaatgccgcgtccggaccgccttcgtagacgattttcatcgcgctctgtaggcgcgccggcatcgtagacgctgaggtggtcaactgatcttcgccccgctcctcggcgtctgcctcgctagcttgctgctcaagaacagcgccgacggtgaagtagctgattgccatcaacgcataggtcgcgtcacctgccgaaaagccagcatcgcaaaggaagcgaagctgcgcgtcggctttttccatctgcggcgcggctggccgcgtcccggcatgaatacgcgcgccatcgcgataagcgagcaacgcccgtcgaaaactgcatgcattgcccttcaggaacgaacgccagtcgtcgtcatcccttggcgtcgaatgcgtgtgatttatcgtcagcatggcttcggcaagtgcgtcgagcaacgcacgcttgttcttgaaatgccagtagagcgctggctgttgcaccccgaggcgctcagccagtcggcgcgtcgttagaccttccatgcccacgtcgttaagcagttcgagcgcggttcggatcacggcctcgcgttggagcttgttcattcgcgaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z