LOCUS       BBa_I714041             1239 bp    DNA     linear       15-May-2024
DEFINITION  [I714030][E0240]
ACCESSION   BBa_I714041
VERSION     BBa_I714041.1  
FEATURES             Location/Qualifiers
     oriT            1..355
                     /label=BBa_I714030
     RBS             364..376
                     /label=RBS-3\Weak
     RBS             370..373
                     /label=RBS
     misc_feature    364..376
                     /label=BBa_B0032
     RBS             364..376
                     /label=BBa_B0032
     CDS             383..1102
                     /label=GFP protein
     CDS             383..1102
                     /label=BBa_E0040
     stem_loop       1122..1165
                     /label=stem_loop
     misc_feature    1111..1190
                     /label=BBa_B0010
     terminator      1111..1190
                     /label=BBa_B0010
     stem_loop       1206..1225
                     /label=T7 TE
     misc_feature    1232..1232
                     /label=stop
     polyA_site      1226..1239
                     /label=polya
     misc_feature    1199..1239
                     /label=BBa_B0012
     terminator      1199..1239
                     /label=BBa_B0012
ORIGIN
        1 cctctggtga ctttatccgt aaataattta acccactcca caaaaaggct caacaggttg
       61 gtggttctca ccaccaaaag caccacaccc cacgcaaaaa caagtttttg ctgatttttc
      121 tttataaata gagtgttatg aaaaattagt ttctcttact ctctttatga tatttaaaaa
      181 agcggtgtcg gcgcggctac aacaacgcgc cgacaccgtt ttgtaggggt ggtactgact
      241 atttttataa aaaacattat tttatattag gggtgctgct agcggcgcgg tgtgtttttt
      301 tataggatac cgctaggggc gctgctagcg gtgcgtccct gtttgcatta tgaattacta
      361 gagtcacaca ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      421 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      481 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      541 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      601 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      661 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      721 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      781 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      841 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      901 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      961 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     1021 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1081 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
     1141 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1201 acactggctc accttcgggt gggcctttct gcgtttata
//