LOCUS       BBa_I714043             1162 bp    DNA     linear       25-Nov-2024
DEFINITION  [I714031][E0240]
ACCESSION   BBa_I714043
VERSION     BBa_I714043.1  
FEATURES             Location/Qualifiers
     oriT            1..278
                     /label=BBa_I714031
     RBS             287..299
                     /label=RBS-3\Weak
     RBS             293..296
                     /label=RBS
     misc_feature    287..299
                     /label=BBa_B0032
     RBS             287..299
                     /label=BBa_B0032
     CDS             306..1025
                     /label=GFP protein
     CDS             306..1025
                     /label=BBa_E0040
     stem_loop       1045..1088
                     /label=stem_loop
     misc_feature    1034..1113
                     /label=BBa_B0010
     terminator      1034..1113
                     /label=BBa_B0010
     stem_loop       1129..1148
                     /label=T7 TE
     misc_feature    1155..1155
                     /label=stop
     polyA_site      1149..1162
                     /label=polya
     misc_feature    1122..1162
                     /label=BBa_B0012
     terminator      1122..1162
                     /label=BBa_B0012
ORIGIN
        1 gatctcgtct ttctcttcgg ggaacaccgg cacccgcagg tgctgccgcc gccggcggcc
       61 gtgttccttt tcgtcgttct ccatgcctcg cctcgtctct catgccggcg gtagccggct
      121 gcctcgcaga gcaggatgac ccgttgagcg cccccggcgc gaataaggga cagtgaagat
      181 agataaccgg ctcgccggtt agctaacttc acacatcctg cccgccttac ggcgttaata
      241 acaccaagga aagtctacac cagccattac gatttatcta ctagagtcac acaggaaagt
      301 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      361 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      421 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      481 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
      541 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      601 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      661 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      721 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      781 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      841 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      901 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      961 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
     1021 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1081 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1141 ggtgggcctt tctgcgttta ta
//