LOCUS       BBa_I714045             1015 bp    DNA     linear       15-May-2024
DEFINITION  [I714032][E0240]
ACCESSION   BBa_I714045
VERSION     BBa_I714045.1  
FEATURES             Location/Qualifiers
     oriT            1..131
                     /label=BBa_I714032
     RBS             140..152
                     /label=RBS-3\Weak
     RBS             146..149
                     /label=RBS
     misc_feature    140..152
                     /label=BBa_B0032
     RBS             140..152
                     /label=BBa_B0032
     CDS             159..878
                     /label=GFP protein
     CDS             159..878
                     /label=BBa_E0040
     stem_loop       898..941
                     /label=stem_loop
     misc_feature    887..966
                     /label=BBa_B0010
     terminator      887..966
                     /label=BBa_B0010
     stem_loop       982..1001
                     /label=T7 TE
     misc_feature    1008..1008
                     /label=stop
     polyA_site      1002..1015
                     /label=polya
     misc_feature    975..1015
                     /label=BBa_B0012
     terminator      975..1015
                     /label=BBa_B0012
ORIGIN
        1 tttacgctga gatgatagga tgccatcgtg ttttatcccg ctgaagggcg cacgtttctg
       61 aacgaagtga agaaagtcta agtgcgccct gataaataaa agagttatca gggattgtag
      121 tgggatttga ctactagagt cacacaggaa agtactagat gcgtaaagga gaagaacttt
      181 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      241 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      301 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
      361 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
      421 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
      481 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      541 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
      601 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
      661 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
      721 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
      781 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
      841 ggattacaca tggcatggat gaactataca aataataata ctagagccag gcatcaaata
      901 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      961 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//