LOCUS       BBa_I714060             1270 bp    DNA     linear       22-Nov-2024
DEFINITION  [R0010][I714051]
ACCESSION   BBa_I714060
VERSION     BBa_I714060.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     oriT            209..563
                     /label=BBa_I714030
     misc_feature    572..665
                     /label=BBa_J23008
     misc_feature    674..770
                     /label=BBa_J23009
     stem_loop       790..833
                     /label=stem_loop
     misc_feature    779..858
                     /label=BBa_B0010
     terminator      779..858
                     /label=BBa_B0010
     stem_loop       874..893
                     /label=T7 TE
     misc_feature    900..900
                     /label=stop
     polyA_site      894..907
                     /label=polya
     misc_feature    867..907
                     /label=BBa_B0012
     terminator      867..907
                     /label=BBa_B0012
     oriT            916..1270
                     /label=BBa_I714030
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagcc tctggtgact ttatccgtaa ataatttaac
      241 ccactccaca aaaaggctca acaggttggt ggttctcacc accaaaagca ccacacccca
      301 cgcaaaaaca agtttttgct gatttttctt tataaataga gtgttatgaa aaattagttt
      361 ctcttactct ctttatgata tttaaaaaag cggtgtcggc gcggctacaa caacgcgccg
      421 acaccgtttt gtaggggtgg tactgactat ttttataaaa aacattattt tatattaggg
      481 gtgctgctag cggcgcggtg tgttttttta taggataccg ctaggggcgc tgctagcggt
      541 gcgtccctgt ttgcattatg aattactaga gacccaaaag caagaggtga ttctagttgg
      601 tggttaatga aaattaactt acttactaga aatatctcta aaaagccaga ttattaatcc
      661 ggctttacta gagcgggcgg gcgggcgggc gggatccata aaaaaaaaac ccaaaagcaa
      721 gaggtgattc tagttaaaaa aaaaaagctt cccgcccgcc cgcccgcccg tactagagcc
      781 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      841 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      901 gtttatatac tagagcctct ggtgacttta tccgtaaata atttaaccca ctccacaaaa
      961 aggctcaaca ggttggtggt tctcaccacc aaaagcacca caccccacgc aaaaacaagt
     1021 ttttgctgat ttttctttat aaatagagtg ttatgaaaaa ttagtttctc ttactctctt
     1081 tatgatattt aaaaaagcgg tgtcggcgcg gctacaacaa cgcgccgaca ccgttttgta
     1141 ggggtggtac tgactatttt tataaaaaac attattttat attaggggtg ctgctagcgg
     1201 cgcggtgtgt ttttttatag gataccgcta ggggcgctgc tagcggtgcg tccctgtttg
     1261 cattatgaat
//