LOCUS       BBa_I714061             2154 bp    DNA     linear       22-Nov-2024
DEFINITION  [I714060][E0240]
ACCESSION   BBa_I714061
VERSION     BBa_I714061.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     oriT            209..563
                     /label=BBa_I714030
     misc_feature    572..665
                     /label=BBa_J23008
     misc_feature    674..770
                     /label=BBa_J23009
     stem_loop       790..833
                     /label=stem_loop
     misc_feature    779..858
                     /label=BBa_B0010
     terminator      779..858
                     /label=BBa_B0010
     stem_loop       874..893
                     /label=T7 TE
     misc_feature    900..900
                     /label=stop
     polyA_site      894..907
                     /label=polya
     misc_feature    867..907
                     /label=BBa_B0012
     terminator      867..907
                     /label=BBa_B0012
     oriT            916..1270
                     /label=BBa_I714030
     RBS             1279..1291
                     /label=RBS-3\Weak
     RBS             1285..1288
                     /label=RBS
     misc_feature    1279..1291
                     /label=BBa_B0032
     RBS             1279..1291
                     /label=BBa_B0032
     CDS             1298..2017
                     /label=GFP protein
     CDS             1298..2017
                     /label=BBa_E0040
     stem_loop       2037..2080
                     /label=stem_loop
     misc_feature    2026..2105
                     /label=BBa_B0010
     terminator      2026..2105
                     /label=BBa_B0010
     stem_loop       2121..2140
                     /label=T7 TE
     misc_feature    2147..2147
                     /label=stop
     polyA_site      2141..2154
                     /label=polya
     misc_feature    2114..2154
                     /label=BBa_B0012
     terminator      2114..2154
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagcc tctggtgact ttatccgtaa ataatttaac
      241 ccactccaca aaaaggctca acaggttggt ggttctcacc accaaaagca ccacacccca
      301 cgcaaaaaca agtttttgct gatttttctt tataaataga gtgttatgaa aaattagttt
      361 ctcttactct ctttatgata tttaaaaaag cggtgtcggc gcggctacaa caacgcgccg
      421 acaccgtttt gtaggggtgg tactgactat ttttataaaa aacattattt tatattaggg
      481 gtgctgctag cggcgcggtg tgttttttta taggataccg ctaggggcgc tgctagcggt
      541 gcgtccctgt ttgcattatg aattactaga gacccaaaag caagaggtga ttctagttgg
      601 tggttaatga aaattaactt acttactaga aatatctcta aaaagccaga ttattaatcc
      661 ggctttacta gagcgggcgg gcgggcgggc gggatccata aaaaaaaaac ccaaaagcaa
      721 gaggtgattc tagttaaaaa aaaaaagctt cccgcccgcc cgcccgcccg tactagagcc
      781 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      841 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      901 gtttatatac tagagcctct ggtgacttta tccgtaaata atttaaccca ctccacaaaa
      961 aggctcaaca ggttggtggt tctcaccacc aaaagcacca caccccacgc aaaaacaagt
     1021 ttttgctgat ttttctttat aaatagagtg ttatgaaaaa ttagtttctc ttactctctt
     1081 tatgatattt aaaaaagcgg tgtcggcgcg gctacaacaa cgcgccgaca ccgttttgta
     1141 ggggtggtac tgactatttt tataaaaaac attattttat attaggggtg ctgctagcgg
     1201 cgcggtgtgt ttttttatag gataccgcta ggggcgctgc tagcggtgcg tccctgtttg
     1261 cattatgaat tactagagtc acacaggaaa gtactagatg cgtaaaggag aagaactttt
     1321 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     1381 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
     1441 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt
     1501 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat
     1561 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
     1621 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
     1681 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
     1741 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
     1801 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
     1861 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
     1921 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
     1981 gattacacat ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa
     2041 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2101 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//