BBa_I714061
type: ComponentDefinition
persistent identity: BBa I714061
display id: BBa_I714061
version: 1
was generated by: iGEM to SBOL conversion
description: [I714060][E0240]
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:48Z
mutable provenance: [I714060]+[E0240]
owned by: james
owned by: myers
mutable description: This part consist of Promoter, OriT-F, Key, Terminator, OriT-F, Reporter. When conjugation occurs, replication randomly start from the tandem OriT-F site, when start from the later OriT-F site, the replication will be ended till the first OriT-F site. Thus, the reporter and reporter functions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Mingzhi Qu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I714030
component: BBa_R0010
component: BBa_J23009
component: BBa_J23008
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
component: BBa_B0032
sequence annotation: BBa_R0010
sequence annotation: BBa_I714030
sequence annotation: BBa_J23008
sequence annotation: BBa_J23009
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I714061_sequence/1

BBa_I714030
type: Component
persistent identity: component1951583
display id: component1951583
version: 1
top level: BBa_I714061
definition: BBa_I714030
access: public

BBa_R0010
type: Component
persistent identity: component1951576
display id: component1951576
version: 1
top level: BBa_I714061
definition: LacI
access: public

BBa_J23009
type: Component
persistent identity: component1951585
display id: component1951585
version: 1
top level: BBa_I714061
definition: BBa_J23009
access: public

BBa_J23008
type: Component
persistent identity: component1951584
display id: component1951584
version: 1
top level: BBa_I714061
definition: BBa_J23008
access: public

BBa_B0010
type: Component
persistent identity: component1951598
display id: component1951598
version: 1
top level: BBa_I714061
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1951597
display id: component1951597
version: 1
top level: BBa_I714061
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1951600
display id: component1951600
version: 1
top level: BBa_I714061
definition: BBa_B0012
access: public

BBa_B0012
type: Component
persistent identity: component1951588
display id: component1951588
version: 1
top level: BBa_I714061
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1951586
display id: component1951586
version: 1
top level: BBa_I714061
definition: BBa_B0010
access: public

BBa_B0032
type: Component
persistent identity: component1951594
display id: component1951594
version: 1
top level: BBa_I714061
definition: BBa_B0032
access: public

BBa_I714030
type: Component
persistent identity: component1951592
display id: component1951592
version: 1
top level: BBa_I714061
definition: BBa_I714030
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1951576
display id: annotation1951576
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951576/range1951576/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I714061/annotation1951576/range1951576/1
type: Range
persistent identity: range1951576
display id: range1951576
version: 1
top level: BBa_I714061
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_I714030
type: SequenceAnnotation
persistent identity: annotation1951583
display id: annotation1951583
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951583/range1951583/1
component: BBa_I714030

https://synbiohub.org/public/igem/BBa_I714061/annotation1951583/range1951583/1
type: Range
persistent identity: range1951583
display id: range1951583
version: 1
top level: BBa_I714061
direction: direction/reverse
start: 209
end: 563
orientation: inline

BBa_J23008
type: SequenceAnnotation
persistent identity: annotation1951584
display id: annotation1951584
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951584/range1951584/1
component: BBa_J23008

https://synbiohub.org/public/igem/BBa_I714061/annotation1951584/range1951584/1
type: Range
persistent identity: range1951584
display id: range1951584
version: 1
top level: BBa_I714061
direction: direction/reverse
start: 572
end: 665
orientation: inline

BBa_J23009
type: SequenceAnnotation
persistent identity: annotation1951585
display id: annotation1951585
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951585/range1951585/1
component: BBa_J23009

https://synbiohub.org/public/igem/BBa_I714061/annotation1951585/range1951585/1
type: Range
persistent identity: range1951585
display id: range1951585
version: 1
top level: BBa_I714061
direction: direction/reverse
start: 674
end: 770
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1951586
display id: annotation1951586
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951586/range1951586/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I714061/annotation1951586/range1951586/1
type: Range
persistent identity: range1951586
display id: range1951586
version: 1
top level: BBa_I714061
direction: direction/forward
start: 779
end: 858
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1951588
display id: annotation1951588
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951588/range1951588/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I714061/annotation1951588/range1951588/1
type: Range
persistent identity: range1951588
display id: range1951588
version: 1
top level: BBa_I714061
direction: direction/forward
start: 867
end: 907
orientation: inline

BBa_I714030
type: SequenceAnnotation
persistent identity: annotation1951592
display id: annotation1951592
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951592/range1951592/1
component: BBa_I714030

https://synbiohub.org/public/igem/BBa_I714061/annotation1951592/range1951592/1
type: Range
persistent identity: range1951592
display id: range1951592
version: 1
top level: BBa_I714061
direction: direction/reverse
start: 916
end: 1270
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1951594
display id: annotation1951594
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951594/range1951594/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I714061/annotation1951594/range1951594/1
type: Range
persistent identity: range1951594
display id: range1951594
version: 1
top level: BBa_I714061
direction: direction/forward
start: 1279
end: 1291
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1951597
display id: annotation1951597
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951597/range1951597/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I714061/annotation1951597/range1951597/1
type: Range
persistent identity: range1951597
display id: range1951597
version: 1
top level: BBa_I714061
direction: direction/reverse
start: 1298
end: 2017
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1951598
display id: annotation1951598
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951598/range1951598/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I714061/annotation1951598/range1951598/1
type: Range
persistent identity: range1951598
display id: range1951598
version: 1
top level: BBa_I714061
direction: direction/forward
start: 2026
end: 2105
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1951600
display id: annotation1951600
version: 1
top level: BBa_I714061
location: https://synbiohub.org/public/igem/BBa_I714061/annotation1951600/range1951600/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I714061/annotation1951600/range1951600/1
type: Range
persistent identity: range1951600
display id: range1951600
version: 1
top level: BBa_I714061
direction: direction/forward
start: 2114
end: 2154
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_I714030
type: ComponentDefinition
persistent identity: BBa I714030
display id: BBa_I714030
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-F (Origin of Transfer for the F-plasmid nic region)
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:47Z
mutable provenance: Origin-of-transfer nic region excised from Plasmid F genomic DNA.(NCBI:AP001918)
owned by: james
owned by: myers
mutable description: OriT-F (Origin of Transfer for the F-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by F plasmid machinery begins via rolling circle replication.
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This OriT-F is the same as the host cell's native Origin of Transfer, Please compare the sequence with J01002.
star: true
creator: Mingzhi Qu
type: DnaRegion
role: SO:0000724
role: partType/Conjugation
sequence: https://synbiohub.org/public/igem/BBa_I714030_sequence/1

BBa_J23008
type: ComponentDefinition
persistent identity: BBa J23008
display id: BBa_J23008
version: 1
was generated by: iGEM to SBOL conversion
description: [key3c] riboregulator for lock3 variants
created: 2006-07-09T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: J23007
owned by: james
owned by: myers
mutable description: This part has the same homology region to J01122 as J23007 except that unlike J23007, J23008 does not contain the hairpin that was derived from J01129. J23008 anneals linearly to the stem of the hairpin in J01122 with perfect base pairing and destroys the hairpin in the process and reveals the RBS on J01122. When J01122 is transcribed the RNA secondary structure is such that the RBS hairpins with sequence upstream preventing the ribosome from binding and translation from occuring thereby locking up RFP further down stream. When J23008 is introduced into the cell in trans, we suspect that it will unlock the RBS for RFP as described above allowing translation to occur.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 931
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No design considerations
star: true
creator: Kaitlin Davis
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23008_sequence/1

BBa_J23009
type: ComponentDefinition
persistent identity: BBa J23009
display id: BBa_J23009
version: 1
was generated by: iGEM to SBOL conversion
description: [key3d] riboregulator for lock3 variants
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: Overlap extension
owned by: james
owned by: myers
mutable description: Variant of key3 (see part J01129 (key3), J23007(key3b), J23008(key3c)). Part is in pJ23006, so there is a Ptet upstream of the XbaI site to drive expression. Nevertheless, J23009 is still a basic part allowing both prefix and suffix insertions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23009_sequence/1

https://synbiohub.org/public/igem/BBa_I714061_sequence/1
type: Sequence
persistent identity: BBa I714061 sequence
display id: BBa_I714061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagcctctggtgactttatccgtaaataatttaacccactccacaaaaaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcctctggtgactttatccgtaaataatttaacccactccacaaaaaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I714030_sequence/1
type: Sequence
persistent identity: BBa I714030 sequence
display id: BBa_I714030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctctggtgactttatccgtaaataatttaacccactccacaaaaaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23008_sequence/1
type: Sequence
persistent identity: BBa J23008 sequence
display id: BBa_J23008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23009_sequence/1
type: Sequence
persistent identity: BBa J23009 sequence
display id: BBa_J23009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z