LOCUS       BBa_I714077              439 bp    DNA     linear       25-Nov-2024
DEFINITION  [R0010][I714075] ([pLac][Key1][Double Terminator])
ACCESSION   BBa_I714077
VERSION     BBa_I714077.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..302
                     /label=BBa_J01008
     stem_loop       322..365
                     /label=stem_loop
     misc_feature    311..390
                     /label=BBa_B0010
     terminator      311..390
                     /label=BBa_B0010
     stem_loop       406..425
                     /label=T7 TE
     misc_feature    432..432
                     /label=stop
     polyA_site      426..439
                     /label=polya
     misc_feature    399..439
                     /label=BBa_B0012
     terminator      399..439
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagac ccaaatccag gaggtgaatc tagtaggtgg
      241 ttaatgaaaa ttaacttact tactagaaat atctctaaaa agccagatta ttaatccggc
      301 tttactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      361 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      421 gggcctttct gcgtttata
//