LOCUS       BBa_I714945             3248 bp    DNA     linear       26-Dec-2024
DEFINITION  pLX012
ACCESSION   BBa_I714945
VERSION     BBa_I714945.1  
FEATURES             Location/Qualifiers
     misc_feature    1..203
                     /label=T1T2
     RBS             248..262
                     /label=SDY
     misc_feature    263..263
                     /label=start
     CDS             263..967
                     /label=EGFP
     misc_feature    967..967
                     /label=stop
     misc_feature    1028..1230
                     /label=T1T2
     CDS             2264..3195
                     /label=Amp
     misc_feature    229..246
                     /label=MCS
     misc_feature    1494..2082
                     /label=ColE ori
ORIGIN
        1 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
       61 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
      121 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
      181 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttcctgtccc cgggctcgag
      241 tctagaagag gagaaattaa gcatggtaag caagggcgag gagctgttca ccggggtggt
      301 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      361 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      421 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
      481 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      541 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      601 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      661 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      721 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      781 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      841 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
      901 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      961 catggacgag ctgtacaagt aaactagtgt cgaccatgcg agagtaggga actgccaggc
     1021 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1081 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
     1141 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc
     1201 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttcct gtccatatgc
     1261 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     1321 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1381 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1441 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1501 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1561 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1621 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1681 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1741 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1801 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1861 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1921 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1981 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2041 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2101 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2161 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2221 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2281 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2341 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2401 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2461 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2521 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     2581 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2641 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2701 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2761 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2821 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     2881 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2941 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3001 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3061 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3241 ctgacgtc
//