LOCUS       BBa_I7150                979 bp    DNA     linear       10-May-2024
DEFINITION  R0040.B0030.E0044.B0015
ACCESSION   BBa_I7150
VERSION     BBa_I7150.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     misc_feature    84..842
                     /label=BBa_E0044
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      121 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      181 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      241 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      301 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      361 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      421 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      481 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      541 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      601 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      781 tggatgaact atacaaaagg cctgctgcaa acgacgaaaa ctacgctgct gctgtttaat
      841 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttata
//