##gff-version 3
##sequence-region BBa_I715010 1 2846
BBa_I715010	.	promoter	1	55	.	+	0	ID=BBa_I715003;Name=BBa_I715003
BBa_I715010	.	promoter	43	47	.	+	0	ID=annotation1936231;Name=-10 RNAP binding;Parent=BBa_I715003
BBa_I715010	.	sequence_alteration	43	47	.	+	0	ID=annotation1936232;Name=UV5 -10 RNAP recognition region double mutation;Parent=BBa_I715003
BBa_I715010	.	operator	3	19	.	+	0	ID=annotation1936229;Name=lac 01;Parent=BBa_I715003
BBa_I715010	.	operator	26	42	.	+	0	ID=annotation1936230;Name=lac 01;Parent=BBa_I715003
BBa_I715010	.	promoter	20	25	.	+	0	ID=annotation1936228;Name=-35 RNAP binding;Parent=BBa_I715003
BBa_I715010	.	engineered_region	64	2846	.	+	0	ID=BBa_S03723;Name=BBa_S03723
BBa_I715010	.	engineered_region	64	897	.	+	0	ID=BBa_S03709;Name=BBa_S03709;Parent=BBa_S03723
BBa_I715010	.	CDS	82	897	.	+	0	ID=BBa_J31003;Name=BBa_J31003;Parent=BBa_S03709
BBa_I715010	.	CDS	82	897	.	+	0	ID=annotation1884996;Name=KanR Gene;Parent=BBa_J31003
BBa_I715010	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03709
BBa_I715010	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715010	.	engineered_region	906	2846	.	+	0	ID=BBa_S03720;Name=BBa_S03720;Parent=BBa_S03723
BBa_I715010	.	engineered_region	906	2114	.	+	0	ID=BBa_S03710;Name=BBa_S03710;Parent=BBa_S03720
BBa_I715010	.	CDS	924	2114	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_S03710
BBa_I715010	.	primer_binding_site	2095	2114	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_I715010	.	CDS	924	2114	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_I715010	.	primer_binding_site	924	947	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_I715010	.	stop_codon	2112	2114	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_I715010	.	ribosome_entry_site	906	917	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03710
BBa_I715010	.	conserved_region	910	913	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715010	.	engineered_region	2123	2846	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_S03720
BBa_I715010	.	CDS	2141	2821	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_I715010	.	CDS	2141	2815	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I715010	.	engineered_tag	2822	2846	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I715010	.	ribosome_entry_site	2123	2134	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_I715010	.	conserved_region	2127	2130	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I715010
aattgtgagcggataacaattgacattgtgagcggataacaatatgttgagcacatactagagaaagaggagaaatact
agatgagccatattcaacgggaaacgtcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggta
taaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttg
tttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaattta
tgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaac
agcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttg
cattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaata
acggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataa
gcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaa
ttaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcg
gtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtt
tcatttgatgctcgatgagtttttctaatactagagaaagaggagaaatactagatgaaatctaacaatgcgctcatcg
tcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatat
cgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcaccc
gttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacg
cgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgc
ggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttc
ggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcgg
cggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcc
cttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttc
tttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcga
cgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaa
acgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacg
cgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgt
ccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattgg
accgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgcccta
taccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatactagagaaagaggagaa
atactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggt
cacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaag
gtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggc
tgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggt
gttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcc
cgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgc
tctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatg
gctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactaca
ccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatc
gc